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Analysis of Specific Protein and DNA Felated to Organogenesis of Poplar Callus

Research Project

Project/Area Number 63480065
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 林産学
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

SUMIYA Kazuo  Kyoto Univ., Wood Research Inst., Professor, 木材研究所, 教授 (30027156)

Co-Investigator(Kenkyū-buntansha) TANAKA Kunisuke  Kyoto Pref. Univ., Dt. Agri., Associate Professor, 農学部, 助教授 (90027194)
OGAWA Masahiro  Kyoto Univ., Res. Inst. Food Sci., Instructor, 食糧科学研究所, 助手 (10160772)
Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥5,200,000)
Fiscal Year 1989: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1988: ¥4,200,000 (Direct Cost: ¥4,200,000)
KeywordsWoody plant / Poplar / Cultured cell / Organogenesis / Specific protein in differentiation / 分化特異的遺伝子
Research Abstract

This research project aimed to select the specific protein and DNA changing in the coarse of organogenesis from poplar (Populus nigra) callus and to foreknow its organogenesis. The results obtained in this project are summarized as follows:
1. R-protein I, 40 k Da and pI 8.5, the antibody of which had been obtained at the application of this project, was a glycopolypetide with asparagine linked oligosaccharide chain. The identification of DNA coding this protein was difficult. And then it was not applicable to an indicator protein for the organogenesis.
2. A soluble protein, 31 kDa and pI 8.0, was synthesized in callus and not in the differentiation of shoot primodium. This protein, therefore, might be an indicator for the initiation of differentiation of shoot primodium.
3. The other soluble protein, 33 kDa and pI 6.9, was accumulated in poplar callus during organogenesis, while it was undetectable in poplar bud, petiol and leaf. This protein was also found in shoot primodium of hybrid poplar, but it disappeared in the formation of callus. This protein was not found in shoot primodium of Eucatyptus and hinokiasunaro. So, his protein could ba an indicator for the differentiation of shoot primodium in poplar callus.
4. The identification of DNA coding the last two proteins remained in future.

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] 角谷和男: "Plant Regeneration from Long-Term Cultured Callus of Poplar (Populus nigra)" 木材学会誌. 34. 354-358 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 浅田隆之: "In Vitro Multiple Bud Formation from Shoot Tips of Thujopsis dolabrata var.hondai" 木材学会誌. 35. 1042-1047 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 浅田隆之: "A Specific Polypeptide in Poplar Callus Decreased during Plant Fegeneration" Proceedings of the 6th International Congress of SABRAO. 877-880 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 福西伸一: "ポプラカルスの不定芽分化過程における2、3の特性変化" 木材学会誌. 36. 241-245 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 浅田隆之: "Changes in Soluble Protein Synthesis during Organogenesis in Poplar(Populus nigra L.)Callus" Plant and cell Physiology(投稿予定). 31. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 浅田隆之: "A Protein Accumulated during Organogenesis in Poplar Callus-A Possible Indicator Protein for Organogenesis" Plant and cell Physiology(投稿予定). 31. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kazuo SUMIYA: "Plant Regeneration from Long-Term Cultured Callus of Poplar (Populus nigra)" Mokuzai Gakkaishi, Vol.34, p.354-358 (1988).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Takayuki ASADA: "In Vitro Multiple Bud Formation from Shoot Tips of Thujopsis dolabrata var. hondai" Mokuzai Gakkaishi, Vol.35, p.1042-1047 (1989).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Takayuki ASADA: "A Specific Polypeptide in Poplar Callus Decreased during Plant Regeneration" Proceeding of the 6th International Congress of SABRAO, p.877-880 (1989).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Shin-ichi FUKUNISHI: "Some Characteristic Changes during Bud Formation from Poplar Callus" Mokuzai Gakkaishi, Vol.36, p.241-245 (1990).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Takayuki ASADA: "Changes in Soluble Protein Synthesis during Organogenesis in Poplar (Populus nigra L.) Callus" Plant and Cell Physiology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Takayuki ASADA: "A Protein Accumulated during Organogenesis in Poplar Callus-A Possible Indicator Protein for Organogenesis" Plant and Cell Physiology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 浅田隆之: "In Vitro Multiple Bud Formation from Shoot Tips of Thujopsis dolabrata var.hondai" 木材学会誌. 35. 1042-1047 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 浅田隆之: "A Specific Polypeptide in Poplar Callus Iecreased during Plant Iegeneration" Proceedings of the 6th International Congress of SABRAO. 877-880 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 福西伸一: "ポプラカルスの不定芽分化過程における2、3の特性変化" 木材学会誌. 36. 241-245 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 浅田隆之: "A Protein Accumulated during Organogenesis in Poplar Callus-A Possible Indicator Protein for Organogenesis" Plant and Cell Physiology(投稿予定). 31. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 浅田隆之: "Changes in Soluble Protein Synthesis during Organogenesis in Poplar(Populus nigra L.)Callus" Plant and Cell Physiology(投稿予定). 31. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 伴野規博: 第39回日本木材学会大会研究発表要旨集. 246 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 福西伸一: 第39回日本木材学会大会研究発表要旨集. 247 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 浅田隆之: 第39回日本木材学会大会研究発表要旨集. 248 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 砂川匡: 第39回日本木材学会大会研究発表要旨集. 249 (1989)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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