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A Study on the Relation Between the Activity of Drug-Metabolizing Enzymes and the Duration of Drugs in Fishes

Research Project

Project/Area Number 63480073
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Fisheries chemistry
Research InstitutionKyushu University

Principal Investigator

KOBAYASHI Kunio  Kyushu Univ., Fac. of Agr. ; Professor, 農学部, 教授 (30038195)

Co-Investigator(Kenkyū-buntansha) IMADA Nobuyoshi  Kyushu Univ., Fac. of Agr. ; Assistant Professor, 農学部, 助手 (60038247)
Project Period (FY) 1988 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥5,900,000 (Direct Cost: ¥5,900,000)
Fiscal Year 1990: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1989: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1988: ¥4,100,000 (Direct Cost: ¥4,100,000)
KeywordsFishes / Liver / Drug-metabolizing enzymes / Drugs / Drug-metabolism / 薬物代謝 / 養殖魚 / 医薬品の代謝 / 薬物代謝酵素の活性
Research Abstract

The study has been performed to elucidate the relation between the activity of drug-metabolizing enzymes and the duration of drugs in various cultured fishes.
(1) The optimum temperature for the drug-metabolizing enzymes in yellowtail, red sea bream, ayu, eel, carp, rainbow trout, tilapia and sturgeon varied depending on their inhabitable water temperature, while the optimum pH value for the enzymes did not show any clear tendency among the fishes.
(2) The activity of drug-metabolizing enzymes, especially aryl hydrocarbon hydroxylase (AHH) in yellowtail, rainbow trout and carp varied seasonally.
(3) Although the activities of enzymes in fishes were generally low compared with those in rat, the activities of some enzymes, especially AHH, were easily induced by PCB and some drugs.
(4) The high relationship between the duration of oxolinic acid (OA) in the fishes and their AHH and glucuronic acid transferance activities was observed, whereas no relationship for thiamphenicol (TP) and oxytetracycline (OTC).
(5) The maximum OA level in the tissues of PCB-treated carp which had a high AHH activity induced by 18 times decreased approx. 1/3 that in control, and the duration period of OA in the tissues was also shortened by half.
This investigation proposes an estimation method for the effective dosage of drugs and the duration of drugs in fishes by the determination of their drug-metabolizing enzyme activity, as an economical and speedy method.

Report

(4 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • 1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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