| Budget Amount *help |
¥5,900,000 (Direct Cost: ¥5,900,000)
Fiscal Year 1990: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1989: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1988: ¥4,100,000 (Direct Cost: ¥4,100,000)
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| Research Abstract |
The study has been performed to elucidate the relation between the activity of drug-metabolizing enzymes and the duration of drugs in various cultured fishes.
(1) The optimum temperature for the drug-metabolizing enzymes in yellowtail, red sea bream, ayu, eel, carp, rainbow trout, tilapia and sturgeon varied depending on their inhabitable water temperature, while the optimum pH value for the enzymes did not show any clear tendency among the fishes.
(2) The activity of drug-metabolizing enzymes, especially aryl hydrocarbon hydroxylase (AHH) in yellowtail, rainbow trout and carp varied seasonally.
(3) Although the activities of enzymes in fishes were generally low compared with those in rat, the activities of some enzymes, especially AHH, were easily induced by PCB and some drugs.
(4) The high relationship between the duration of oxolinic acid (OA) in the fishes and their AHH and glucuronic acid transferance activities was observed, whereas no relationship for thiamphenicol (TP) and oxytetracycline (OTC).
(5) The maximum OA level in the tissues of PCB-treated carp which had a high AHH activity induced by 18 times decreased approx. 1/3 that in control, and the duration period of OA in the tissues was also shortened by half.
This investigation proposes an estimation method for the effective dosage of drugs and the duration of drugs in fishes by the determination of their drug-metabolizing enzyme activity, as an economical and speedy method.
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