| Project/Area Number |
63480090
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
基礎獣医学
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| Research Institution | Hokkaido University |
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Principal Investigator |
SUGIMURA Makoto Hokkaido Univ., Fac. of Vet. Med. Professor, 獣医学部, 教授 (40001523)
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| Co-Investigator(Kenkyū-buntansha) |
KON Yasuhiro Hokkaido Univ., Fac. of Vet. Med. Instructor, 獣医学部, 助手 (10178402)
HASHIMOTO Yoshiharu Hokkaido Univ., Fac. of Vet. Med. Associate Professor, 獣医学部, 助教授 (20091481)
SUZUKI Yoshitaka Gifu Univ., Fac. of Agr. Professor, 農学部, 教授 (70021699)
北川 浩 北海道大学, 獣医学部, 助手 (40125307)
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| Project Period (FY) |
1988 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1990: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1989: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1988: ¥3,700,000 (Direct Cost: ¥3,700,000)
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| Keywords | Immunohistochemistry / Lymphatic tissue / Lymph node / Macrophage / Reticular cell / Reticuloendothelial system / S-100 protein / Spleen / 脾臓 / 免疫組織化学 / リンパ組織 / S-100蛋白質 |
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| Research Abstract |
The aim of this study is to clarify the morphology and function of subsets of accessory cells belonging to the reticuloendothelial system (RES) by use of immunohistochemical markers, such as S-100 protein.
S-100 immunoreactivity was detected in follicular dendritic cells (FDC) of germinal center of all mammalian species observed, and also in macrophages, interdigitating reticulum cells and endothelial cells of lymphatic sinus (SEC) with species-differences of its intensities. It was also noted that FD
C and SEC were positive to S-100 alpha monoclonal antibody. This shows S-100 alpha subunit is useful as a special marker for the both cells. By use of the marker, it was suggested that FDC did not induce the formation of germinal centers, because S-100 positive FDC appeared after formation of lymph
oblastic clusters in the solid lymphatic nodule. In the RES of healthy chickens, S-100 positive cells were generally not detected, but in a few chickens with various diseases, FDC-like cells in splenic germinal centers and follicles of bursa of Fabricius contained S-100 protein. Further studies need for resolving why S-100 protein increases in such conditions.
In a part of comparative studies, splenic macrophages of the red sea-bream, macrophages and antibody-producing cells in the carp head kidney, and macrophages in the chick epididymis were studied to clarify their roles in immune response or in sperm removal.
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