| Project/Area Number |
63480097
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Fukuoka University School of Medicine |
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Principal Investigator |
YAMADA Eichi Fukuoka University School of Medicine Department of Anatomy, Professor, 医学部, 教授 (30037311)
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| Project Period (FY) |
1988 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1990: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1989: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1988: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Ciliary Epithelium / Aqueous humor / Na / K ATPase / Electron microscopy / intraepithelial nerve / isolated epithelium / 毛様体突起 / 上皮内神経線維 / 眼房水産生 / 神経終末 |
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| Research Abstract |
The fine structure of ciliary epithelium was investigated by various techniques of electron microscopy. The iridial portion of ciliary process in rabbit eye was mainly used, where the aqueous humor production is supposed to be most active.
1. The epithelium is consisted of pigmented (PE) and nonpigmented (NPE) epithelial layer. The basolateral cell surfaces of both PE and NPE epitheliocyte show elaborate infoldings which interdigitate with those of adjacent epitheliocytes, and their cell membrane contains abundant intramembrane particles in the freeze fractured specimen.
2. The intraepithelial nerve fibers were found. They showed beaded appearance and distributed among PE epitheliocytes. They contain 2 kinds of vesicle in their dilated portion. The smaller vesicles (-50 nm in diameter) contain dense granule after treatment with 5' -hydroxydopamine, thus indicated thus indicated their noradrenergic nature.
3. The topical application of forskolin induced following morphological changes ; oedemous stroma of ciliary process, and dilation of ciliary channel as well as intercellular space of PE. The findings suggest that the agent caused the reabsorption of the aqueous.
4. The intact ciliary epithelial bilayer (PE+NPE) was successfully isolated by perfusion method using collagenase solution. The immunocytochemical localization of Na/K ATPase was investigated on the isolated fresh bilayer specimen. The EM observation revealed that the enzyme localizes on the cell membrane along the basolateral surfaces of both PE and NPE. The findings suggest both cell layers participate in aqueous humor production.
5. The physiological and pharmacological experiment is underway using this isolated epithelial sheet and promising results are already obtained.
These experiments were collaborated with Prof. M. Sears, Yale University School of Medicine.
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