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Analysis of Mechanisms for the Formation of Nerve Terminal Sprouts

Research Project

Project/Area Number 63480105
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Neurophysiology and muscle physiology
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

KUNO Motoi  Kyoto Univ. Med. Fac. Professor, 医学部, 教授 (50142295)

Co-Investigator(Kenkyū-buntansha) YAO Hiromu  Kyoto Univ. Med. Fac. Instructor, 医学部, 助手 (00144353)
TAKAHASHI Tomoyuki  Kyoto Univ. Med. Fac. Ass. Prof., 医学部, 講師 (40092415)
Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1989: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1988: ¥5,400,000 (Direct Cost: ¥5,400,000)
KeywordsMotoneuron / Neuromuscular junction / Sprouting / Plasticity / Muscle / Peptide / Axonal transport / 運動ニューロン
Research Abstract

Sprouting of nerve terminals has been assumed to be a morphological correlate of plastic changes in functional synaptic transmission. To test this assumption, we induced sprouting of motor nerve terminals in the rat extensor digitorum longus muscle by chronic conduction block of the sciatic nerve with tetrodotoxin. The amount of transmitter release at these neuromuscular junctions was measured electrophysiologically in terms of the mean quantum content of end-plate potentials., When the nerve conduction had been blocked for 6 days, about 35% of the motor nerve terminals examined in the hind leg muscle showed sprouting. After measurements of transmitter release by electrophysiological experiments, each muscle fiber was stained with fluorescent dye, and the same neuromuscular junctions were used for morphometric observations. Following a conduction block of the sciatic nerve for 6 days, all the neuromuscular junctions showed a significant increase in the amount of transmitter release uni … More formly, regardless of the presence or absence of terminal sprouts. The total terminal length in the junctions with sprouts was significantly greater than that in those without sprouts. However, there was no correlation between the terminal length and the amount of transmitter release. It is concluded that terminal sprouting and increased transmitter release are not causally related.
We have previously shown that the formation of terminal sprouts at neuromuscular junctions is significantly inhibited by treatment with calcitonin gene-related peptide (CGRP). This suggests that CGRP may be transported from the neuron cell body to its terminal in which CGRP may act as a neurotrophic factor. To test this possibility, we developed a highly sensitive enzyme immunoassay for CGRP. With this assay, the CGRP content in the sciatic nerve was found to be about 100 pg/mm. Also, about 95% of CGRP detected in skeletal muscle was found to originate from neural tissues. In both the peripheral sensory and motor nerve fibers, CGRP was found to transport in the somatofugal direction at a rate of about 1 mm/hr. Less

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] TSUJIMOTO,T.&KUNO,M.: "Calcitonin gene-related peptide prevents disuse-induced sprouting of rat moter nerve terminals." Journal of Neuroscience. 8. 3951-3957 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] KASHIHARA,Y.,SAKAGUCHI,M.&KUNO,M.: "Axonal transport of CGRP in the rat peripheral nerve." Neuroscience Research. Suppl.9. S124 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] KUNO,M.,TSUJIMOTO,T.&UMEMIYA,M.: "Disparity of terminal sprouting and functional plasticity induced by prolonged disuse at neuromuscular junctions." Biomedical Research. Suppl.2. 155-157 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] MANABE,T.,KANEKO,S.&KUNO,M.: "Disuse-induced enhancement of Ia synaptic transmission in spinal motoneurons of the rat" Journal of Neuroscience. 9. 2455-2461 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] KASHIHARA,Y.,SAKAGUCHI,M.&KUNO,M.: "Axonal transport and distribution of endogenous calcitonin gene-related peptide in rat peripheral nerve." Journal of Neuroscience. 9. 3796-3803 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] TSUJIMOTO,T.,UMEMIYA,M.&KUNO,M.: "Terminal sprouting is not responsible for enhanced transmitter release at disused neuromuscular junctions of the rat." Journal of Neuroscience(印刷中). 10. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] KUNO,M.: "Target-dependence of the survival and electrophysiological properties of spinal motoneurons in the mammal.In:The Current Status of Peripheral Nerve Regeneration." Alan R.Liss,Inc.,New York, 11 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] KUNO,M.&TSUJIMOTO,T.: "Terminal sprouting and functional plasticity at neuromuscular junctions.In:Brain Signal Transduction and Memory." Academic Press,Inc.,Tokyo, 10 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] TSUJIMOTO, T. & KUNO, M.: "Calcitonin gene-related peptide prevents disuse-induced sprouting of rat motor nerve terminals." Journal of Neuroscience 8: 3951-3957, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] KUNO, M.: "Target-dependence of the survival and electrophysiological properties of spinal motoneurons in the mammal." IN: The Current Status of Peripheral Nerve Regeneration, Alan R. Liss, Inc. New York, pp. 3-13, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] KASHIHARA, Y., SAKAGUCHI, M. & KUNO, M.: "Axonal transport of CGRP in the rat peripheral nerve." Neuroscience Research Suppl. 9: S124, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] KUNO, M., TSUJIMOTO, T. & UMEMIYA, M.: "Disparity of terminal sprouting and functional plasticity induced by prolonged disuse at neuromuscular junctions." Biomedical Research Suppl. 2: 155-157, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] MANABE, T., KANEKO, S. & KUNO, M.: "Disuse-induced enhancement of Ia synaptic transmission in spinal motoneurons of the rat." Journal of Neuroscience 9: 2455-2461, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] KASHIHARA, Y., SAKAGUCHI, M. & KUNO, M.: "Axonal transport and distribution of endogenous calcitonin gene-related peptide in rat peripheral nerve." Journal of Neuroscience 9: 3796-3802, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] KUNO, M. & TSUJIMOTO, T.: "Terminal sprouting and functional plasticity at neuromuscular junctions." In: Brain Signal Transduction and Memory, Academic Press, Inc., Tokyo, pp. 259-268, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] TSUJIMOTO, T., UMEMIYA, M. & KUNO, M.: "Terminal sprouting is not responsible for enhanced transmitter release at disused neuromuscular junctions of the rat." Journal of Neuroscience 10, 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] TSUJIMOTO,T.& KUNO,M.: "Calcitonin gene-related peptide prevents disuse-induced sprouting of rat motor nerve terminals." Journal of Neuroscience. 8. 3951-3957 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] KASHIHARA Y.SKAGUCHI,M.& KUNO,M.: "Axonal transport of CGRP in rat perpheral nerve." Neuroscience Research. Suppl.9. S124 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] KUNO M.,TSUJIMOTO,T.& UMEMIYA,M.: "Disparity of terminal sprouting and functional plasticity induced by prolonged disuse at neuromuscular junctions." Biomedical Research. Suppl.2. 155-157 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] MANABE,T.,KANEKO,S.& KUNO,M.: "Disuse-induced enhancement of Ia the rat." Journal of Neuroscience. 9. 2455-2461 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] KASHIHARA,Y.,SAKAGUCHI,M.& KUNO,M.: "Axonal transport and distribution of endogneous calcitonin gene-related peptide in rat peripheral nerve." Journal of Neuroscience. 9. 3796-3802 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] TSUJIMOTO,T.UMEMIYA,M.& KUNO,M.: "Terminal sprouting is not responsible for enhanced transmitter release at disused neuromuscular junctions of the rat." Journal of Neuroscience. 10. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] KUNO,M.: "Target-dependence of the survival and electrophysiological properties of spinal motoneurons in the mammal.In:The Current Status of Peripheral Nerve Regeneration." Alan R.Liss,Inc.,New York, 11 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] KUNO,M.& TSUJIMOTO,T.: "Terminal sprouting and functional plasticity at neuromuscular junctions.In:Brain Signal Transducition and Memory." Academic Press,Inc.,Tokyo, 10 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kuno,M.;Tsujimoto,T.: Brain Signal Transduction and Memory,ed.by M.Ito & Y.Nishizuka,Academic Press. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kuno,M.;Tsujimoto,T.;Umemiya,M.: Neurosci.Res.9. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Tsujimoto,T.;Umemiya,M.;Kuno,M.: Japanese J.Physiol.39. (1989)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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