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Quantitative analysis of the electrogenic ion exchange current using the cell dialysis method in the isolated cardiac myocytes.

Research Project

Project/Area Number 63480106
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Neurophysiology and muscle physiology
Research InstitutionKyushu University

Principal Investigator

NOMA Akinori  Kyushu Univ. Fac. of Med. Professor, 医学部, 教授 (00132738)

Co-Investigator(Kenkyū-buntansha) MITSUIYE Tamotsu  Kyushu Univ. Fac. of Med. Research Associate, 医学部, 助手 (40174065)
Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1989: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1988: ¥3,000,000 (Direct Cost: ¥3,000,000)
Keywordssingle cardiac myocyte / Na / Ca exchange / ion exchange / intracellular Ca / patch clamp technique / Na@Ca交換機転 / 単一心室筋細胞 / イオンフラックス / Na-Ca交換機転
Research Abstract

The intracellular Ca^<2+> concentration is lower by 10^4 than the extracellular Ca^<2+> concentration. This concentration gradient is essential to achieve various kinds of cell functions, such as the muscle contraction, and electrical activity of the cell. The Na/Ca exchange mechanism is mainly responsible for pumping out Ca^<2+> which enters the cardiac muscle during the systolic period. The aim of our project is to determine the stoichiometry of the Na/Ca exchange by recording the Na/Ca exchange current. We clarified that the intracellular ion concentrations may change, if we stimulate the Na/Ca exchange continuously. Therefore, we used a holding potential which is equal to the theoretical equilibrium potential for the 3Na:1Ca exchange. The reversal potential of the Na/Ca exchange current were successfully measured at various external Na^+ and Ca^<2+> concentrations. The reversal potential was in agreement with the theoretical values of the 3Na:1Ca exchange.

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Matsuoka S,Ehara T.,Noma A.: "Reversal potential of the Na-Ca exchange current in the cardiac myocyte of the guinea pig." Biomedical Research.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Suppl.2: "9" 1988. 149-152.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ehara T.,Matsuoka,S.,Noma A.: "Measurement of reversal potential of Na^+-Cas^<2+> exchange current in single guinea-pig ventricular cells." Journal of Physiology. 410. 227-249 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Matsuoka S., Ehara T., Noma A.: "Reversal potential of the Na-Ca exchange current in the cardiac myocyte of the guinea pig." Biomedical Research 9(Suppl. 2):149-152 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ehara T., Matsuoka S., Noma A.: "Measurement of reversal potential of Na^+-Ca^<2+> exchange current in single guinea-pig ventricular cells." Journal of Physiology 410:227-249 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Matsuoka S.,Ehara T.,Noma A.: "Reversal potential of the Na-Ca exchange current in the cardiac myocyte of the guinea pig." Biomedical Research. 9(Suppl.2). 149-152 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Ehara,T.,Matsuoka S.,Noma A.: "Measurement of reversal potential of Na^+ーCa^<2+> exchange current in single guinea-pig ventricular cells." Journal of Physiology. 410. 227-249 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Ehara T.;Matsuoka S.;Noma A.: Journal of Physiology. in press. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Matsoka S.;Ehara T.;Noma A.: Biomedical Research. 9. 149-152 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Matsuda H.;Matsuura H.;Noma A.: Journal of Physiology. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Noma A.;Ehara T.: "Electrogenic Na-K pump and Na-Ca exchange in cardiac muscle.In ISOLATED ADULT CARDIOMYOCYTES" G.Isenberg, (1989)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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