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Macro- and microscopic analysis of glycine-gated response in isolated CNS neurons.

Research Project

Project/Area Number 63480107
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Neurophysiology and muscle physiology
Research InstitutionTOHOKU UNIVERSITY (1989)
Kyushu University (1988)

Principal Investigator

AKAIKE Norio  Tohoku University School of Medicine, Professor, 医学部, 教授 (30040182)

Co-Investigator(Kenkyū-buntansha) OYAMA Yasuo  Tohoku University School of Medicine, Assistant Researcher, 医学部, 助手 (80214229)
NAKAYE Toshio  Tohoku University School of Medicine, Assistant Researcher, 医学部, 助手 (20155659)
津田 泰夫  九州大学, 医学部, 助手 (20036495)
池本 清海  九州大学, 医学部, 講師 (90091272)
Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 1989: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1988: ¥3,200,000 (Direct Cost: ¥3,200,000)
Keywordsrat / hypothalamus / isolated neuron / glycine / Cl^- response / strychnine / NMDA / T-type Ca channel / 視床下部腹内側角 / 孤束核 / 脊随後角 / N-メチル-D-アスパラギン酸 / 単離ニューロン / Cl応答 / 用量反応曲線 / 交叉脱感作 / ストリキニーネ
Research Abstract

Pharmacological properties and kinetics of glycine-induced currents were investigated in single ventromedial hypothalamic (VMH) neurons acutely isolated from young and adult rats by the use of a 'concentration-clamp' technique, which allows both internal perfusion and rapid application of an external solution under single-electrode voltage-clamp. The glycine-induced current reversed at the Cl^- equilibrium potential (E_<Cl>), and a ten-fold change of extracellular Cl^- concentration resulted in a 53 mV shift of the glycine reversal potential. Glycine-induced Cl^- currents increased sigmoidally in a concentration-dependent manner with a K_d of 9 x 10^<-5> M at the Hill coefficient of 1.8. The glycine-induced conductance exhibited a striking voltage dependency at membrane potentials more negative than -50 mV and reached a steady state value when hyperpolarized beyond -110 mV. Both the activation and inactivation phases of glycine-induced response are described by double exponential funct … More ions (fast and slow components, respectively) with the concentrations used. All four time constants decreased with increasing glycine concentration. The blockade of the glycine response by strychnine and picrotoxin was noncompetitive. The responses to individual alpha- and (bata-amino acids were selectively antagonized by strychnine, but were not affected by bicuculline or a taurine antagonist, TAG, suggesting that alpha-and beta-amino acids activate the same glycine receptor. The pharmacological properties of low-threshold (T-type) Ca channels of VMH neurons for organic Ca blockers differed from those of peripheral ganglion cells and cultured neurons. The GABA-induced Cl^- current in rat isolated Purkinje cells was also investigated by concentration-clamp technique. The pharmacological property of GABA receptor was basically similar to that previously reported in other preparations. In isolated nucleus tructus solitarii neurons, glycine initiates the activation of NMDA receptors rather than that glycine prevents the desensitivation at NMDA receptors in the cells. Less

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Kaneda,M.: "GABAーinduced chloride current in rat isolated Purkinje cells." Am.J.Physiol.256. 1153-1159 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Tokutomi,N.: "What confers specificity on glycine for its receptor site?" Br.J.Pharmacol.97. 353-360 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Yakushiji,T.: "Effects of benzodiazepines and nonーbenzodiazepine compounds on the GABAーinduced response in frog isolated sensory neurones." Br.J.Pharmacol.98. 735-740 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Akaike,N.: "Glycineーgated chloride current in acutely isolated rat hypothalamic neurons." J.Neurophysiol.62. 1400-1409 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Akaike,N.: "Dihydropyridineーsensitive lowーthreshold calcium channels in isolated rat hypothalamic neurones." J.Physiol.London. 412. 181-195 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Shirasaki,T.: "Glycineーinsensitive desensitization of NーmethylーDーaspartate receptors in acutely isolated mammalian central neurons." Neurosci.Lett.(In press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kaneda, M.: "GABA-induced chloride in rat isolated Purkinje cells." Am. J. Physiol. 256; 1153-1159 (1989).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Tokutomi, N.: "What confers specificity on glycine for its receptor site?" Br. J. Pharmacol. 97; 353-360 (1989).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Yakushiji, T.: "Effects of benzodiazepines and non-benzodiazepine compounds on the GABA-induced response in frog isolated sensory neurons." Br. J. Pharmacol. 98; 735-740 (1989).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Akaike, N.: "Glycine-gated chloride current in acutely isolated rat hypothalamic neurons." J. Neurophysiol. 62; 1400-1409 (1989).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Akaike, N.: "Dihydropyridine-sensitive low-threshold calcium channels in isolated rat hypothalamic neurons." J. Physiol.(London) 412; 181-195 (1989).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Shirasaki, T.: "Glycine-insensitive desensitization of N-methyl-D-aspartate receptors in acutely isolated mammalian central neurons." Neurosci. Lett. 108; 93-98 (1990).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Tokutomi,N.: "What confers specificity on glycine for its receptor site.?" Br.J.pharmacol.97. 353-360 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kaneda,M.: "GABA-induced chloride current in rat isolated purkinje cell." Am.J.physiol.256. c1153-c1159 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Akaike,N.: "Dihydropyridine-sensitive low-threshold calcinm channels in isolated rat hypothalamic neurones." J.physiol.412. 181-195 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Akaike,N.: "Glycine-gated chloride current in acutely isolated rat hypothalamic neurons." J.Neurophsiol.62. 1400-1409 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Shirasaki,T.: "Glycine-insensitive desensitization of N-methyl-D-aspartate receptors in acutely isolated mammalian central neurons." Neurosci.Lett.

    • Related Report
      1989 Annual Research Report
  • [Publications] Nakagawa,T.: "Effects of antagonists on N-methyl-D-aspartate response in acutely isolated nucleus tractus solitarii neurons of the rat." Neurosci.Lett.

    • Related Report
      1989 Annual Research Report
  • [Publications] Akaike,N.: "Chloride Channels and Carries in Nerve,Muscle and Glial Cells.“GABA-gated c1^-currents and their regulation by intracellular free Ca^<2+>"." plenum press, 11

    • Related Report
      1989 Annual Research Report
  • [Publications] Akaike,N.: "Vision,Temporal Lobe,and Memory.“Modulation of NMDA receptors in isolated rat CNS neurons by the competitive and non-competitive drugs and 5-HT"." Elsevier-USA-Japan Science, (Inpress)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kaneda,M.: Neurosci.Res.5. 299-315 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Ikemoto,Y.: Pfluger Arch.412. 240-247 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Hori,N.: J.Neurosci.Method. 25. 197-208 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Akaike,N.: J.Pkysiol.(London).

    • Related Report
      1988 Annual Research Report
  • [Publications] Tokutomi,N.: Br.J.Pharmacol.

    • Related Report
      1988 Annual Research Report
  • [Publications] Akaike,N.: J.Neurophysiology.

    • Related Report
      1988 Annual Research Report
  • [Publications] Kostyuk,P.: "Calcium channels:Structure and function,"Gating and permeation of different types of Ca channels"" The New York Academy of Science,

    • Related Report
      1988 Annual Research Report
  • [Publications] Akaike,N.: "Ion channels and intracellular regulatory mechanisms."Electrical and pharmacological properties of isolated rat CNS neurons"" Biomedical Research Foundation Press,

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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