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Synaptic Transmission and Modulation in the Autonomic Nervous System

Research Project

Project/Area Number 63480109
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Neurophysiology and muscle physiology
Research InstitutionKurume University

Principal Investigator

NISHI Syogoro  Kurume U. Sch. Med., Professor, 医学部, 教授 (00080557)

Co-Investigator(Kenkyū-buntansha) TANAKA Eiichiro  Kurume U. Sch. Med., Instructor, 医学部, 助手 (80188284)
MIHARA Satoshi  Kurume U. Sch. Med., Instructor, 医学部, 助手 (40166103)
INOKUCHI Hiroe  Kurume U. Sch. Med., Asst. Professor, 医学部, 講師 (10080558)
YOSHIMURA Megumu  Kurume U. Sch. Med., Asst. Professor, 医学部, 講師 (10140641)
HIGASHI Hideho  Kurume U. Sch. Med., Assoc. Professor, 医学部, 助教授 (10098907)
矢野 秀樹  久留米大学, 医学部, 助手 (60210308)
Project Period (FY) 1988 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1990: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1989: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1988: ¥5,100,000 (Direct Cost: ¥5,100,000)
KeywordsSpinal lateral horn / Fast EPSP / Slow EPSP and IPSP / Glutamate receptors / Adrenoceptors / Signal transduction / Protein kinase C / G protein / 脊髄 / 側角細胞 / 興奮性アミノ酸 / 速い経過のシスプス電位 / ノルアドレナリン / 神経伝達物質 / プロテインキナ-ゼC / 脊髄側角ニューロン / 速および緩徐興奮性シナプス電位 / 緩徐抑制性シナプス電位 / アルファ受容体 / Ca依存性Kコンダクタンス / 消化管内在神経細胞 / ムスカリン性受容体
Research Abstract

1. Measurement of the release of transmitters mediating the fast EPSP (glutamate and aspartate), the slow EPSP (noradrenaline) and the slow IPSP (noradrenaline) in the spinal lateral horn cells (cat) : The spontaneous and evoked release of glutamate and aspartate from tissue slices of the lateral hoorn were measured by the gass-mass spectrometry. The release of noradrenaline from the same tissue preparation was measured by a high-pressure liquid chromatography.
2. Characterization of the receptors mediating the synaptic potentials in the lateral horn cells (cat) and analysis of the intracellular signal transduction for the slow PSPs : The glutamate receptors responsible for production of the fast EPSP were a non-NMDA type in 70% of the cells and non-NMDA plus NMDA types in 30% of the cells. Most of the fast IPSP were mediated by glycine via glycine receptors, while some of the fast IPSP were mediated by GABA through GABA-A receptors. The slow EPSP and IPSP were evoked by noradrenaline via alpha-1 and alpha-2 adrenoceptors, respectively. The former was associated with closing of Ca-dependent K channels due to activation of protein kinase C, and the latter was accompanied by opening of voltage-independent K channels due to activation of G protein.
3. Presynaptic inhibition in the lateral horn cells (cat) : The fast EPSP in these cells were inhibited presynaptically by noradrenaline. This inhibition was due to a lowering of the probability of glutamate release.
4. Intracellular signal transduction that produces the slow IPSP in Meissner's neurons of alimental canal (guinea-pig) : It was found that the outward synaptic current evoked by the transmitter noradrenaline in Meissner's neurons were produced not only by opening of K channels triggered by activated G protein but also by activation of Ca-dependent K channels that occurred simultaneously with the former.

Report

(4 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • Research Products

    (47 results)

All Other

All Publications (47 results)

  • [Publications] Syogoro Nishi: "Electrophysiological properties of sympathetic preganglionic neurons." Annual Review of Physiology. 50. 541-551 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Syogoro Nishi: "Enhancement of peptidergic synaptic transmission by catecholamines in the bullfrog sympathetic ganglion." Journal of Autonomic Nervous System. 23. 95-102 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Satoshi Mihara: "Muscarinic M_2 receptors on cardiac ganglion neurons of the guineaーpig heart" The Kurume Medical Journal. 35. 183-192 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Satoshi Mihara: "Muscarinic excitation and inhibition of neurons in the submucous plexus of the guineaーpig caecum" Neuroscience. 31. 247-257 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Syogoro Nishi: "Multiple actions of noradrenaline on sympathetic preganglionic neurons of the cat studied in the spinal cord slice" Progress in Brain Research. 81. 181-190 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Syogoro Nishi: "On synaptic transmission to preganglionic neurones" Proceedings of the International Union of Physiological Sciences. 17. 461-S5075 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Satoshi Mihara: "Signal transduction mechanisms underlying the slow synaptic potentials in neurons ot the submucous plexus of the guineaーpig cecum" Proceedings of the International Union of Physiological Sciences. 17. 484-485 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Syogoro Nishi: "Vibration stress and the cellular mechanisms in the autonomic nervous system" The Kurume Medical Journal. 37. S23-S32 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Syogoro Nishi: "Catecholaminergic modulation and transmission in sympathetic preganglionic neurons" The Japanese Journal of Physiology. 40. 775-787 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hiroe Inokuchi: "The effects of 5ーhydroxytryptamine on cat sympathetic preganglionic neurons in vitro" The Kurume Medical Journal. 37. 309-312 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Satoshi Mihara: "Mechanisms underlying intracellular signal transduction of the slow IPSP in submucous neurones of the guineaーpig caecum" The Journal of Physiology. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hiroe Inokuchi: "Fast excitatory postーsynaptic potentials and the responses to excitant amino acids of sympathetic preganglionic neurons in the slice of the cat spinal cord" Neuroscience.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Syogoro Nishi: "New Aspects in Regional Anaestesia" Verlag Fur Medizin Dr.Ewald Fischer (Heidelberg), 149-158 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 西 彰五郎: "新生理科学大系:第20巻 内分泌・自律機能調節の生理学" 廣重 力、佐藤 昭夫 編 医学書院, 460 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Syogoro Nishi: "Electrophysiological properties of sympathetic preganglionic neurons" Annual Review of Physiology. 50. 541-551 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Syogoro Nishi: "Enhancement of peptidergic synaptic transmission by catecholamines in the bullfrog sympathetic ganglion" Journal of Autonomic Nervous System. 23. 95-102

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Satoshi Mihara: "Muscarinic M_2 receptors on cardiac ganglion neurons of the guinea-pig heart" The Kurume Medical Journal. 35. 183-192 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Satoshi Mihara: "Muscarinic excitation and inhibition of neurons in the submucous plexus of the guinea-pig caecum" Neuroscience. 31. 247-257 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Syogoro Nishi: "Multiple actions of noradrenaline on sympathetic preganglionic neurons of the cat studied in the spinal cord slice" Progress in Brain Research. 81. 181-190 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Syogoro Nishi: "On synaptic transmission to preganglionic neurones" Proceedings of the International Union of Physiological Sciences. 17. 461-S5075 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Satoshi Mihara: "Signal transduction mechanisms underlying the slow synaptic potentials in neurons of the submucous plexus of the guinea-pig cecum" Proceedings of the International Union of Physiological Sciences. 17. 484-485 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Syogoro Nishi: "Vibration stress and the cellular mechanisms in the autonomic nervous system" The Kurume Medical Journal. 37. S23-S32 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Syogoro Nishi: "Catecholaminergic modulation and transmission in sympathetic preganglionic neurons" The Japanese Journal of Physiology. 40. 775-787 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hiroe Inokuchi: "The effects of 5-hydroxytrypta-mine on cat sympathetic preganglionic neurons in vitro" The Kurume Medical Journal. 37. 309-312 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Satoshi Mihara: "Mechanisms underlying intra-cellular signal transduction of the slow IPSP in submucous neurones of the guinea-pig caecum" The Journal of Physiology. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hiroe Inokuchi: "Fast excitatory post-synaptic potentials and the responses to excitant amino acids of sympathetic preganglionic neurons in the slice of the cat spinal cord" Neuroscience.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Syogoro Nishi: Verlag fur Medizin Dr. Ewald Fischer (Heidelberg). New Aspects in Regional Anaesthesia, 149-158 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Syogoro Nishi: "Vibration stress and the cellular mechanisms in the autonomic nervous system" The Kurume Medical Journal. 37. S23-S32 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Syogoro Nishi: "Catecholaminergic modulation and transmission in sympathetic preganglionic neurons" The Japanese Journal of Physiology. 40. 775-787 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hiroe Inokuchi: "The effects of 5ーhydroxytryptamine on cat sympathetic preganglionic neurons in vitro" The Kurume Medical Journal. 37. 309-312 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Satoshi Mihara: "Mechanisms underlying intracellular signal transduction of the slow IPSP in submucous neurones of the guineaーpig caecum" The Journal of Physiology. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hiroe Inokuchi: "Fast excitatory postーsynaptic potentials and the responses to excitant amino acids of sympathetic preganglionic neurons in the slice of the cat spinal cord" Neuroscience.

    • Related Report
      1990 Annual Research Report
  • [Publications] 西 彰五郎: "新生理科学大系:第20巻 内分泌・自律機能調節の生理学" 廣重 力,佐藤 昭夫編 医学書院, 460 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Satoshi Mihara: "Muscarinic Excitation and Inhibition of Neurons in the Submucous Pelxus of the Guineaーpig Caecum" Neuroscience. 31. 247-257 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Syogoro Nishi: "Multiple Actions of Noradrenaline on Sympathetic Preganglionic Neurons of the Cat Studied in the Spinal Cord Slice" Progress in Brain Research. 81. 181-190 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Satoshi Mihara: "Muscarinic M_2 Receptors on Cardiac Ganglion Neurons of the Guineaーpig Heart" The Kurume Medical Journal. 35. 183-192 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Syogoro Nishi: "On Synaptic Transmission to Preganglionic Neurones" Proceedings of the International Union of Physiological Science. 17. 461-S5075 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Satoshi Mihara: "Signal Transduction Mechanisms Underlying the Slow Synaptic Potentials in Neurons of the Submucous Plexus of the Guineaーpig Cecum" Proceedings of the International Union of Physiological Sciences. 17. 484-485 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Syogoro Nishi: "Intracellular Signal Transduction Mechanisms Underlying the Slow Inhibitory Postsynaptic Potential in Neurones of the Submucous Plexus of the Guienaーpig Caecum" Journal of Physiology(London).

    • Related Report
      1989 Annual Research Report
  • [Publications] 西彰五郎: "新生理科学大系 第20巻:内分泌・自律機能調節の生理学" 医学書院,

    • Related Report
      1989 Annual Research Report
  • [Publications] Syogoro Nishi.: Annual Review of physiology. 50. 541-551 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Syogoro Nishi.: Journal of Autonomic Nervous System. 23. 95-102 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Syogoro Nishi.: The Kurume Medical Journal. 35. (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Syogoro Nishi.: Neuroscience. (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Syogoro Nishi.: Progress in Brain Research. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Syogoro Nishi.: "New Aspects in Regional Anaesthesia" Verlag fur Medizin Dr.Ewald Fischer, 149-158 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 西 彰五郎: "新生理科学大系 第20巻:内分泌・自律機能調節の生理学" 医学書院,

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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