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Specificity of function of acetylcholinergic receptors in the caudate and vestibular nuclei.

Research Project

Project/Area Number 63480119
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General pharmacology
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

TAKAORI Shuji  Fac. of Med., Kyoto Univ. Professor, 医学部, 教授 (10025538)

Co-Investigator(Kenkyū-buntansha) ISHIHARA Kumatoshi  Fac. of Med., Kyoto Univ. Instructors, 医学部, 助手 (20212912)
UJIHARA Hisamitsu  Fac. of Med., Kyoto Univ. Instructors, 医学部, 助手 (00213421)
HARA Mitsuyoshi  Fac. of Med., Kyoto Univ. Instructors, 医学部, 助手 (50192282)
AKAIKE Akinori  Fac. of Med., Kyoto Univ. Lecturer, 医学部, 講師 (80135558)
SASA Masahi  Fac. of Med., Kyoto Univ. Associate Prof., 医学部, 助教授 (20025654)
Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥5,800,000 (Direct Cost: ¥5,800,000)
Fiscal Year 1989: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1988: ¥4,100,000 (Direct Cost: ¥4,100,000)
KeywordsCaudate nucleus / Vestibular nucleus / Acetylcholine / Muscarinic receptors / Dopamine receptor / Intracellular recording
Research Abstract

Electrophysiological studies, using slice preparation of the rat brain, were undertaken to examine the regulatory mechanism of acetylcholine receptors in the caudate and vestibular nuclei. Either carbachol or milscarine dosedependently inhibited extracellular action potentials orthodromically elicited by local stimulation in the caudate nucleus. A combination of acetylcholine with physostigmine also inhibited the orthodromic responses, but nicotine had no effects on the neuronal activity. Carbachol or muscarine decreased the amplitude of excitatory postsynaptic potential and increased firing induced by intracellular depolarizing pulsesinto the cells, whereas nicotine did not affect these potentials. The inhibitory and excitatory effects of carbachol were blocked by atropine. These results suggest that muscarinic receptors located on the presynaptic and postsynaptic sites of the neuron mediate cholinergic inhibition and excitation in the caudate nucleus, respectively.
Bromocriptine, a dopamine D-2 agonist, produced a depolarization of the membrane potential of the caudate neurons, and an increase in the spontaneous firing rate as well as the number of action potentials evoked by depolarizing pulses applied into the cells. The bromocriptine-induced excitation was antagonized by domperidone (D-2 antagonist) and haloperidol, but not affected by SCH 23390 (D-1 antagonist). The medial vestibular nucleus neurons fired spikes spontaneously. Carbachol dose-dependently increased the spontaneous firing, and the addition of atropine attenuated the carbachol-induced excitation of the neurons. Acetylcholine in the presence of physostigmine also increased the firing rate, while nicotine had no effects. These results suggest that firing rate of the spontaneously active neurons in the medial vestibular nucleus are regulated by acetylcholine via muscarinic receptors.

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Akaike,A.: "Muscarinic inhibition as adominant role in cholinergic regulation of transmission in the caudate nucleus." J Pharmacol Exp Ther. 246. 1129-1136 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ujihara,H.: "Electrophysiological evidence for cholinoceptive neurons in the medial vestibular nucleus:studies on rat brain stem in vitro." Neurosci Lett. 93. 231-235 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ujihara,h.: "Muscarinic regulation of spontaneously active medial vestibular neurons in vitro." Neurosci Lett. 106. 205-210 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Akaike,A.: "Voltage- and current-clamp studies on muscarinic and nicotinic excitation of the rat adrenal chromaffin cells." J Pharmacol Exp Ther:発表予定.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Akaike,A.: "A Patch-clamp study on muscarinic and nicotinic excitation of the rat adrenal chromaffin cells." J Pharmacol Exp Ther:発表予定.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ohno,Y.: "Further evidence that dopamine D-2 receptor mediates excitation of caudate nucleus neurons:intracellular studies in vitro." Life Sci:発表予定.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Akaike A, Sasa M, Takaori S: "Muscarinic inhibition as a dominant role in cholinergic regulation of transmission in the caudate nucleus." J Pharmacol Exp Ther 246, 1129-1136 (1988).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ujihara H, Akaike A, Sasa M, Takaori S: "Electrophysiological evidence for cholinoceptive neurons in the medial vestibular nucleus: studies on rat brain stem in vitro." Neurosci Lett 93, 231-235 (1988).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ujihara H, Akaike A, Sasa M, Takaori S: "Muscarinic regulation of spontaneously active medial vestibular neurons in vitro." Neurosci Lett 106, 205-210 (1989).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Akaike A, Mine Y, Sasa M, Takaori S: "Voltageand Current-clamp studies on muscarinic and nicotinic excitation of the rat adrenal chromaffin cells." J Pharmacol Exp Ther.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Akaike A, Mine Y, Sasa M, Takaori S: "A patch-clamp study on muscarinic and nicotinic excitation of the rat adrenal chromaffin cells." J Pharmacol Exp Ther.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ohno Y, Akaike A, Sasa M, Takaori S: "Further evidence that dopamine D-2 receptor mediates excitation of caudate nucleus neurons: intracellular studies in vitro." Life Sci.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ujihara,H.: "Muscarinic regulation of spontaneously active vestibular neurons in vitro." Neurosci Lett. 106. 205-210 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Hara,M.: "Ventral tegmental area-mediated inhibition of neurons of the nucleus accumbens receiving input from the parafascicular nucleus of the thalamus is mediated by dopamine D_1 receptors." Neuropharmacology. 28. 1203-1209 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Hirose,A.: "Inhibition of hippocampal CA1 neurons by 5-hydroxy-tryptamine derived from the dorsal raphe nucleus and the5-hydroxytryptamine_<1A> agonist SM-3997." Neuropharmacology. 29. 93-101 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Akaike,A.: "Voltage-and current-clamp studies on muscarinic and nicotinic excitation of the rat adrenal chromaffin cells." J Pharmacol Exp Ther.

    • Related Report
      1989 Annual Research Report
  • [Publications] Akaike,A.: "A patch-clamp study on muscatinic and nicotinic excitation of the rat adrenal chromaffin cells." J Pharmacol Exp Ther.

    • Related Report
      1989 Annual Research Report
  • [Publications] Ohno,Y.: "Further evidence that dopamine D-2 receptor mediates excitation of caudate nucleus neurons:intracellular studies in vitro." Life Sci.

    • Related Report
      1989 Annual Research Report
  • [Publications] Akaike,A.: J Pharmacol Exp Ther. 246. 1129-1136 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Nabatame,H.: Japan J Pharmacol. 46. 387-395 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Ujihara,H.: Neurosci Lett. 93. 231-235 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Ujihara,H.: Neurosci Lett.

    • Related Report
      1988 Annual Research Report
  • [Publications] Akaike,A.: J Pharmacol Exp Ther.

    • Related Report
      1988 Annual Research Report
  • [Publications] Akaike,A.: J Pharmacol Exp Ther.

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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