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Purification and Functional Analysis of Adfesion Related Molecules Between Hematopoietic Stem Cells and Marrow Stromal Cells.

Research Project

Project/Area Number 63480141
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionChiba University

Principal Investigator

HARIGAYA Kenichi  Chiba University, School of Medicine, Associate Professor, 医学部, 助教授 (40101894)

Co-Investigator(Kenkyū-buntansha) HANDA Hiroshi  University of Tokyo, Faculty of Medicine, Associate Professor, 医学部, 助教授 (80107432)
Project Period (FY) 1988 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1990: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1989: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1988: ¥3,900,000 (Direct Cost: ¥3,900,000)
KeywordsMarrow stromal cell line / Hematopoietic stem cells / Monoclonal antibodies / adhesion related molecule (s) / ヒト骨髄間質細胞株 / 細胞膜接着分子 / 骨髄間質細胞株 / 造血細胞増殖分化
Research Abstract

In order to investigate novel adhesion molecule (s) in stromal hematopoiesis, we raised monoclonal antibodies (MAb) against human marrow stromal cell lines KM-101 and KM-102 and myeloid hematopoietic cell line (CMK). The antibodies were screened by the direct addition into coculture plates containing KM cell line and K562. One of the antibodies enhanced the attachment between K562 and stromal cell lines. This antibody recognized the 135-Kilodalton membrane molecules and its Fab fragment markedly inhibited the adhesion between stromal cells and K562 cells. Immunohistochemical staining of this Mab revealed positive reaction in stromal cells of wide variety of tissues and vascular endothelia, smooth muscle cells, and some of epithelial cells including renal collecting tubules, colon and pancreatic duct. No positive reaction was found in neural cells or skeletal muscles. The distribution of the molecules are similar to CD44 antigen with a few exception, but the size of the molecules are much different. Direct binding of ^<125>I on the surface membrane of various kind of blood cells showed that peripheral white blood cells had low radiolabelling while relatively immature hematopoieticcell lines incorporated twice or more radioactive antibodies. The amount of the direct binding was modulated when the hematopoietic cell lines were treated with 10ng/ml TPA 3 days in advance. These results indicates that some sosts of exogenous stimuli modulate the expression of 135 K dalton membrane molecule. Taking together with the distribution of the molecules among a wide varieties of stroma and parenchyma, the molecules recognized by Mab C-F-9 may be one of signaling molecules between parenchymal and stromal signal transduction, as well as an adhesion related protein.

Report

(4 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Umezawa,A,.Harigaya,k.et al.: "Gapーjunctional communication of bone marrow stromal cells is resistant to irradiation in vitro" Experimental Hematology. 18. 1002-1007 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hayakawa,T.et al.: "Tissue in hibitor of metalloproteinases from human bone marrow stromal cell live KM102 has erythroidーpotentiating activity, suggesting its possibly bifunctional role in hematopoietic microcnvironment." FEBS Letters. 268. 125-128 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Harigaya,K.: "Bone marrow stromal cells and hematopoiesis. Im Childhood Leukenia:Present Problems and Future Prospects." Kruer Acad.Publ.,Boston,

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kohama, T, et al.: "A burst-promoting activity from the human bone marrow stromal cell line KM-102 is identical to the granulocyte-macrophage colony-stimulating factor." Exp. Hematol. 16. 603-608 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] FitzGerald, T. J. et al.: "Radiosensitivity of permanent human bone marrow stromal cell lines : effect of dose rate." Int. J. Rad. Oncol. Biol. Phys. 15. 1153-1159 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ohkawa, H. et al.: "Novel function and cellular interaction of human lymphoid stromal cells with lymphoid cell lines in vitro." Exp. Hematol. 17. 30-33 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Holland, C. A. et al: "Differential expression of the neomycin resistance gene transferred by a retroviral vector to hematopoietic and stromal cells in human continuous bone marrow cultures." Acta Haematol.82. 136-143 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Holland, C. A. et al.: "Differentiation of human hematopoietic cells increases expression of a gene transferred by a retroviral vector." J. Leu. Biol.46. 221-229 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Umezawa, a. et al.: "Gap-junctional communication of human marrow stromal cells is resistant to irradiation in vitro" Exp. Hematol.18. 1002-1007 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hayakawa, T. et al: "Tissue inhibitor of metalloproteinases from human bone marrow stromal cell line KM102 has erythroid potentiating activity, suggesting oits possibly bifunctional role in the hematopoietic microenvironment." FEBS Letters. 268. 125-128 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Harigaya, K. et al.: Kruer Acad. Publ., Boston.Bone marrow stromal cells and hematopoiesis. in Childhood Leukemia ; Present Problems and Future Prospects. eds Kobayashi, N et al.,

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Umezawa,A.,Harigaya,K.et.al.: "Gapーjunctional communication of bone marrow stromal cells is resistant to irradiation in vitro." Experimental Hematology. 18. 1002-1007 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hayakawa,T.et al.: "Tissue inhibitor of metallo proteinases from human bone mauow stromal cell line KM102 has erythroidーpotentrating activity,suggesting its possibly bifunctional role in hematopoietic microenvironment" FEBS Letters. 268. 125-128 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Harigaya,K.: "Bone marrow stromal cells and hematopoiesis. In Childhood Leukemia:Presont Problems and Future Prospcts." Kruer Acacl,Publ.,Boston,

    • Related Report
      1990 Annual Research Report
  • [Publications] H.Ohkawa et al.: "Novel function and cellular interaction of human lymphoid stromal cells with lymphoid cell lines in vitro." Experimental Hematology. 17. 30-33 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 張ヶ谷健一他: "造血支持細胞の機能的・形態的多様性" 細胞. 22. 84-88 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Hayakawa et al.: "Tissue inhibitor of metalloproteinases from human bone stromal cell line KM 102 has erythroid potentiaing activity-possible bifunctional role in hemopoietic.." Nature.

    • Related Report
      1989 Annual Research Report
  • [Publications] Klhama.T;Handa.H;Harigaya.K: Experimental Hematology. 16. 603-608 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] FitzGerald.TJ;Santucc.MA;Harigaya.K;et al.: International Journal Radiation Oncology Bioloby Physics. 15. 1153-1159 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Ohkawa.H;Mikata.A;Harigaya.K;Ueda.R: Experimental Hematology. 17. 30-33 (1989)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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