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Application of Monoclonal Antibody for Diagnosis and Treatment of Alveolar Hydatid Disease

Research Project

Project/Area Number 63480148
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 寄生虫学(含医用動物学)
Research InstitutionAsahikawa Medical College

Principal Investigator

KATSUMI Haruhiko  Asahikawa Medical College, Professor, 医学部, 教授 (90072868)

Co-Investigator(Kenkyū-buntansha) INAOKA Thoru  Asahikawa Medical College, Assistant, 医学部, 助手 (00091562)
NAKAO Minorru  Asahikawa Medical College, Assistant, 医学部, 助手 (70155670)
Project Period (FY) 1988 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥5,800,000 (Direct Cost: ¥5,800,000)
Fiscal Year 1990: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1989: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1988: ¥3,400,000 (Direct Cost: ¥3,400,000)
KeywordsAlveolar Hydatid Disease / Monoclonal Antibody / Diagnosis / Treatment / モノクローナル抗体 / 診断と治療
Research Abstract

Current biotechnology improved diagnostic and tools in parasitic diseases. In this study, murine monoclonal antibodies were produced to apply for the diagnosis and treatment of alveolar hydatid disease (AHD) caused by larval Echinococcus multilocularis.
Spleen cells from infected or immunized Balb/c mouse were fused with myeloma cells (P3X63-Ag8.653) by polyethylene glycol, and the hybridomas were grown in serum-free medium. ABC (avidin-biotin peroxidase complex) method using paraffin sections of alveolar hydatid was used as a screening assay. The hybridomas secreting antibodies specific for brood capsules, protoscolices or germinal layers were cloned by limiting dilution.
Fab fragments of the monoclonal antibody specific for germinal layers were radiolabeled with 131-I, and then injected into mice and rats bearing hepatic alveolar hydatids. However, effective images of the hepatic lesions were not obtained, probably because the antibody could not enter the inside of hydatids through the laminated layers. These results suggest that immunotoxins may be inapplicable for the treatment of AHD. In contrast, the immunohistological detection of germinal layers with monoclonal antibody was effective for evaluating the viable sites of hydatids in human hepatic lesions.
Antigenic characteristics in the Hokkido isolates of E. Multilocularis were examined by immunoblotting. The patient sera mainly reacted with two proteins of which molecular weights were 65 and 56-kilodalton (kDa). These antigens were different from the 54kDa antigens, designated "Em2" in the European isolate, and were nonreactive with sera of other parasitic infections. Accordingly, the production of monoclonal antibodies against these antigens is necessary to purify the immunodiagnostic antigens.

Report

(4 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 久津見 晴彦: "多包虫症" 最新医学. 44(4). 889-894 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] NAKAO M, NAKAYA K, KUTSUMI H.: "Murine model for hepatic alveolar hydatid disease without biohazard" Jpn.J.Parasitol.39(3). 296-298 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] NAKAO M, KUTSUMI H.: "Hokkaido isolate of <Echinococcus>___ー <multilocularis>___ー: sterile hydatids,protoscolices and adults share the cmmon antigen" Manuscript in preparation.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] NAKAO M, KUTSUMI H.: "Production of murine monoclonal antibodies against the Hokkaido isolate of <Echinococcus>___ー <multilocularis>___ー" Manuscript in preparation.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] KUTSUMI H.: "Alveolar Hydatid Disease" Saishin-Igaku. 44(4). 889-894 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] NAKAO M, NAKAYA K, KATSUMI, H.: "Murine model for hepatic alveolar hydatid disease without biohazard" Jpn. J. Parasitol.39(3). 296-298 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] NAKAO M, KUTSUMI H.: "Hokkido isolate of Echinococcus multilocularis : sterile hydatids, protoscolices and adults share the common antigen"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] NAKAO M, KUTSUMI H.: "Production of murine monoclonal amtibodies against the Hokkaido isolate of Echinococcus multilocularis"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 久津見 晴彦: "多包虫症" 最新医学. 44(4). 889-894 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] NAKAO M,NAKAYA K,KUTSUMI H.: "Murine model for hepatic alveolar hydatid disease without biohazard" Jpn.J.Parasitol.39(3). 296-298 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] NAKAO M,KUTSUMI H.: "Hokkaido isolate of <Echinococcus>___ー <multilocularis>___ー:sterile hydatids,protoscolices and adults share the common antigen" Manuscript.

    • Related Report
      1990 Annual Research Report
  • [Publications] NAKAO M,KUTSUMI H.: "Production of murine monoclonal antibodies against the Hokkaido isolate of <Echinococcus>___ー <multilocularis>___ー" Manuscript.

    • Related Report
      1990 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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