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Structure of Receptor Binding Domain of Neurovirulent Virus.

Research Project

Project/Area Number 63480160
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Virology
Research InstitutionOsaka University

Principal Investigator

UEDA Shigeharu  Research Institute for Microbial Diseases, Osaka University, Department of Preventive Medicine, Proressor, 微生物病研究所, 教授 (90068453)

Co-Investigator(Kenkyū-buntansha) OKUNO Yoshinobu  Research Institute for Microbial Diseases, Osaka University, Department of Preve, 微生物病研究所, 助教授 (30112064)
Project Period (FY) 1988 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1990: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1989: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1988: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordsmeasles virus / neurovirulence / hemagglutinin / H protein / receptor binding domain / 脳炎 / 麻疹ウイルスH遺伝子 / エピト-プ / レセプター結合ドメイン
Research Abstract

To investigate viral factors concerning neurovirulence of a virus, we focused receptor binding domains of the virus on the role in the expression of neurovirulence. A strain of measles virus (the Nagahata strain), which was isolated from a throat swab obtained from a typical measles patient using a primary culture of human embryonic kidney cells, was propagated and plaque-purified in Vero cells. The virus was named as N-V strain. A variant strain named as N-HB was obtained after 4 times passages in brains of suckling golden damsters. Only 250 PFU of the N-HB strain injected into the brain of adult golden hamsters caused a neurological disease characterized by hyperactivity, myoclonic convulsions, generalized convulsions, and lethargy in the late stage. To analyze differences of receptor binding domains between the N-V and N-HB strain, H protein-coding gene of both strains were cloned and sequences. The gene of both strains have 1851 nucleotides. The homology of the nucleotide sequence of H gene between both strains is 99.5%. Nucleotides differ at 10 positions. The H protein of both strains has 617 amino acids which are predicted from the nucleotide sequence. The homology of the amino acid sequence of H protein between both strains is 98.9%. Amino acids differ at 7 positions : Leu at position 43 of the H protein of N-V strain was substituted with Phe in that of the N-HB strain. Likewise, amino acid substitutions were as follows : Thr (107) ->Ala, Ser (126) ->Asn, Ile (308) ->Phe, Leu (321) ->Pro, Ser (370) ->T h r, Phe (431) ->Leu.
The amino acid substitution at position 321, Leu ->Pro has possibility to change the secondary structure of the H protein and seems important for expression of neurovirulence.

Report

(4 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] Y.Tamaka,M.Watanabe,Y.Isegawa,Y.Okuno,and S.Ueda: "A neurovirulent variant of measles virus has a different nucleotide sequence in the H gene from that of its nonneurovirulent parent strain."

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yoshikazu Tanaka, Yuji Isegawa, Michiko Watanabe, Yoshinobu Okuno and Shigeharu Ueda.: "A neurovirulent variant of measles virus has a different nucleotide sequence in the gene of H protein from that of the non-neurovirulent parent strain."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Wong,T.C.,Ayata,M.,Ueda,S.,and Hirano,A.: "Role of biased hypermutation in evolution of subacute sclerosing panencephalitis virus from progenitor acute measles virus." J.Virol.(1991)

    • Related Report
      1990 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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