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Analysis of the Biological Function of CD5 Molecule.

Research Project

Project/Area Number 63480170
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Immunology
Research InstitutionKyushu University

Principal Investigator

NISHIMURA Yasuharu  Department of Genetics, Medical Institute of Bioregulation, Kyushu University, Associate Professor, 生体防御医学研究所, 助教授 (10156119)

Co-Investigator(Kenkyū-buntansha) KIMURA Akinori  Department of Genetics, Medical Institute of Bioregulation, Kyushu University, R, 生体防御医学研究所, 助手 (60161551)
Project Period (FY) 1988 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 1990: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1989: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1988: ¥2,800,000 (Direct Cost: ¥2,800,000)
KeywordsCD5 molecule / T cell activation / Mutant cell / Retroviral vector / Gene transfection / Interleukin 1 / 突然変異細胞 / トランスフェクション / CD5 / CD5欠損突然変異株 / ILー1 / ILー1受容体 / IL-1 / IL-1受容体 / T細胞レセプター / CD3複合体 / Jurkat細胞株 / 欠損変異株 / 組み換えレトロウィルス
Research Abstract

In order to define biological function of the CD5 (T1, Leu1, Tp67) molecule, a cDNA clone of CD5 was expressed in a CD5 deficient Jurkat cell line and in a murine T cell hybridoma. A Jurkat subclone (Jurkat 9.9) produced interleukin-2 (IL-2) in response to anti-CD3 monoclonal antibody (MAB) crosslinked to solid support. IL-2 production was enhanced by co-culture with the anti-CD5 MAb OKT1. A CD5 deficient mutant clone Jurkat 1.15 was generated by treatment with ethyl methanesulfonate followed by selection with anti-CD5 MAb plus complement. Jurkat 1.15 did not demonstrate enhancement of IL-2 production by OKT1 in the presence of crosslinked anti-CD3 MAb. A cDNA encoding CD5 was introduced into a defective retrovirus which was used to infect Jurkat 1.15. A Jurkat clone stably expressing CD5 was established. In response to OKT1, a rise in intracellular calcium was observed in both the parent Jurkat 9.9 and the CD5 positive infectant but not in the CD5 negative mutant or a G418 resistant c … More ontrol. Furthermore, expression of CD5 restored the augmentation of Il-2 production by OKT1 in response to crosslinked anti-CD3 MAb. A murine T cell hybridoma By155.16 which produces IL-2 in response to HLA-DR antigens was also infected with the CD5 recombinant retrovirus and three stable CD5 positive infectants were generated. These hybridomas showed enhancement of Il-2 production by stimulation with OKT1 in the presence of suboptimal concetrations of soluble anti-murine CD3 MAb. These results provide further evidence that CD5 provides a costimulatory signal for T cell activation.
The role of the CD5 surface molecule in T cell responsiveness to interleukin-1 (IL-1) was examined. The CD5+ wild type Jurkat 9.9 produced interleukin-2 (IL-2) in response to anti-CD3 monoclonal antibocy (MAb), OKT3, crosslinked to a solid surface. IL-2 production was enhanced by co-culture with IL-1 or anti-CD5 MAb. Neither the CD5- mutant nor the CD5- G418-resistant infectant responded to anti-CD5 MAb or to IL-1. Responsiveness to IL-1 was restored by cell surface expression of CD5 in the CD5+ infectant. Both the CD5+ wild type Jurkat and the CD5+ infectant responded equivalent to purified IL-1, recomvinant IL-1alpha and recobinant IL-1beta. Optimal concentrations of IL-1and anti-CD5 MAb had an additive effect upon the enhancement of IL-2 production stimulated with crosslinked anti-CD3 MAb suggesting that IL-1 and CD5 act through distinct pathways. The specific binding of recombinant IL-1beta was examined in these cell lines. Both the specific binding (at 4゚C) and subsequent internalization (at 37゚C) of 125I labeled recombinant IL-1beta was equivalent in the CD5+ infectant and the CD5+ wild type Jurkat cell, whereas specific binding of ^<125>I labeled recombinant I1-1beta was markedly decreased in the CD5- G418-resistant infectant. These observations strongly suggest that cell surface expression of CD5 regulates binding of and responsiveness to IL-1. Less

Report

(4 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • Research Products

    (72 results)

All Other

All Publications (72 results)

  • [Publications] Nishimura,Y.: "Expression and function of a CD5 cDNA in human and murine T cells." European Journal of lmmunolgy. 18. 747-753 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Nishimura,Y.: "Expression of CD5 regulates responsiveness to interleukinー1." Journal of Immunolgy. 141. 3438-3444 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Bierer,B.E.: "Phenotypic and functional characterization of human cytolytic T cells lacking expression of CD5." Journal of Clinical Investigation. 81. 1390-1397 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sasazuki,T.: "Differential roles of HLAーDR and DQ in immune regulation" Progress in Immunology Vll, Springer verlag, Berlin. 7. 853-860 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Honda,K.: "Hashimoto thyroiditis and HLA in Japanese" Clinical Endocrinology and Metabolism. 69. 1268-1273 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sasazuki,T.: "HLAーlinked immune suppression in humans." Immunology,Supplement. 2. 21-24 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Fukui,Y.: "Glycosuria and insulitis in NOD mice expressing the HLAーDQw6 molecule" Journal of Immunogenetics. 16. 445-453 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sasazuki,T.: "Expression and function of human major histocompatibility complex HLAーDQw6 genes in the C57BL/6 mouse." Cold Spring Harbor Symposium on Quantitative Biology. 54. 513-520 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ottenhoff,T.H.M.: "HLAーDQ molecules and the control of Mycobacterrium Leprae specific T cell nonresponsiveness in lepromatous leprosy patients." European Journal of Immunolgy. 20. 2347-2350 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Nishimura,Y.: "Expression of human major histocompatibility complex,HLAーDQw6 genes alters the immune response in C57BL/6 mice." Journal of Immunology. 145. 353-360 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Nagafuchi,S.: "Reversal of nonーresponders and postexposure prophylaxis by intradermal hepatitis B vaccination in Japanese medical personnel." Journal of American Medecal Association.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kamikawaji,N.: "HLAーDQ resetricted CD4^+ T cells specific to streptococcal antigen exist in low responders but not in high responders." Journal of Immunology.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 西村 泰治: "CD3(T3)複合体" 代謝 25号臨時増刊号「代謝病ハイライト」. 16. 385-389 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 西村 泰治: "ヒトT細胞における付着分子" Medical Immunology. 16. 561-568 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 西村 泰治: "RAの治療指針とHLA" リウマチ. 28. 463-466 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 土屋 邦善: "DNA解析による疾患感受性の検出(RAとSLE)" 内科. 66. 697-698 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 西村 泰治: "分子遺伝学:RA感受性を支配するDRB1遺伝子" 病理と臨床. 8. 1372-1375 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 土屋 邦善: "HLAダイビング" 現代医療. 23. 299-304 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 西村 泰治: "HLAーDQトランスジェニックマウス" 代謝26巻増刊号「免疫 '89」. 26. 159-168 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 西村 泰治: "免疫抑制とMHC" 臨床免疫. 21. 1362-1372 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 西村 泰治: "ヒトの免疫抑制遺伝子" 医学のあゆみ. 151. 582-585 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 西村 泰治: "HLAーDQと免疫抑制" Annual Review免疫 1990. 463-466 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 西村 泰治: "HLAーDQトランスジェニックマウス" 医学のあゆみ. 154. 482 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 西村 泰治: "免疫応答および疾患感受性を支配する遺伝要因" 日本体質学会雑誌. 54. 45-47 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 西村 泰治: "ヒト免疫応答の個体差とその遺伝" アレルギ-の臨床. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 西村 泰治: "RFLP解析を用いたHLAクラスII遺伝子のタイピング" Clinical Immunology Newsletter. 10. 60 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 岡本 安弘: "慢性関節リウマチの理解のために,5遺伝「慢性関節リウマチ」水島裕編集" 南江堂 東京, 237-242 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 西村 泰治: "抗原特異的T細胞増殖反応による免疫抑制遺伝子の解析「生化学実験講座」第12巻分子免疫学II免疫遺伝学・アレルギ-、日本生化学編" 東京化学同人 東京, (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 西村 泰治: "トランスジェニックマウスを用いたMHCの解析「生化学実験講座」第12巻分子免疫学 II免疫遺伝学・アレルギ-、日本生化学会" 東京化学同人 東京, (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Nishimura, Y., Bierer, B. E., Jones, W. K., Jones, N. H., Strominger, J. L., and Burakoff, S. J.: "Expression and function of a CD5 cDNA in human and murine T cells." European Journal of Immunology. 18. 747-753 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Nishimura, Y., Bierer, B. E. and Burakoff, S. J.: "Expression of CD5 regulates responsiveness to interleukin-1." Journal of Immunology. 141. 3438-3444 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Bierer, B. E., Nishimura, Y., Burakoff, S. J. and Smith, B. F.: "Phenotypic and functional characterization of human cytolytic T cells lacking expression of CD5." Journal of Clinical Investigation. 81. 1390-1397 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sasazuki, T., Kamikawaji, N., Fujisawa, K., Yoshizumi, H., Yasunami, M., Kimura, A., and Nishimura, Y.: "Differential roles of HLA-DR and DQ in immune regulation" Progress in Immunology Vll, Springer verlag, Berlin. 7. 853-860 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Honda, K., Tamai, H., Morita, T., Kuma, K., Nishimura, Y., and Sasazuki, T.: "Hashimoto thyroiditis and HLA in Japanese" Clinical Endocrinology and Metabolism. 69. 1268-1273 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sasazuki, T., Kikuchi, I., Hirayama, K., Matsushita, S., Ohta, N., and Nishimura, Y.: "HLA-linked immune suppression in humans." Immunology, Supplement. 2. 21-24 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Fukui, Y., Nishimura, Y., Iwanaga, T., Kimura, A., Inamitsu, T., Hanaoka, Y, Kitagawa, T., and Sasazuki, T.: "Glycosuria and insulitis in NOD mice expressing the HLA-DQw6 molecule" Journal of Immunogenetics. 16. 445-453 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sasazuki, T., Iwanaga, T., Inamitsu, T., Yanagawa, Y., Yasunami, M., Kimura, A. Hirokawa, K., and Nishimura, Y.: "Expression and function of human major histocompatibility complex HLA-DQw6 genes in the C57BL/6 mouse." Cold Spring Harbor Symposium on Quantitative Biology. 54. 513-520 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ottenhoff, T. H. M., Walford, C., Nishimura, Y., Reddy, N. B. B., and Sasazuki, T.: "HLA-DQ molecules and the control of Mycobacterium Leprae specific T cell nonresponsiveness in lepromatous leprosy patients." European Journal of Immunology. 20. 2347-2350 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Nishimura, Y., Iwanaga, T., Inamitsu, T., Yanagawa, Y., Yasunami, M., Kimura, A., Hirokawa, K., and Sasazuki, T.: "Expression of human major histocompatibility complex, HLA-DQw6 genes alters the immune response in C57BL/6 mice." Journal of Immunology. 145. 353-360 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Nagafuchi, S., Kashiwagi, S., Okada, K., Anzai, K., Nakamura, M., Nishimura, Y., Sasazuki, T., and Niho, Y.: "Reversal of non-responders and postexposure prophylaxis by intradermal hepatitis B vaccination in Japanese medical personnel." Journal of American Medical Association.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kamikawaji, N., Fujisawa, K., Yoshizumi, H., Fukunaga, M., Yasunami, M., Kimura, A., Nishimura Y., and Sasazuki, T.: "HLA-DQ restricted CD4^+ T cells specific to streptococcal antigen exist in low responders but not in high responders." Journal of Immunology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sasazuki,T.: "Expression and function of human major histocompatibility complex HLAーDQw6 genes in the C57BL/6 mouse." Cold Spring Harbor Symposium on Quantitative Biology. 54. 513-520 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Ottenhoff,T.H.M.: "HLAーDQ molecules and the control of Mycobacterium Leprae specific T cell nonresponsiveness in lepromatous leprosy patients." European Journal of lmmunology. 20. 2347-2350 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nishimura,Y.: "Expression of human major histocompatibility complex ,HLAーDQw6 genes alters the immune response in C57BL/6 mice." Journal of lmmunology. 145. 353-360 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nagafuchi,S.: "Reversal of nonーresponders and postexposure prophylaxis by intradermal hepatitis B vaccination in Japanese medical personnel" Journal of American Medical Association.

    • Related Report
      1990 Annual Research Report
  • [Publications] Kamikawaji,N.: "HLAーDQ resetricted CD4^+ T cells specific to streptococcal antigen exist in low responders but not in high responders." Journal of lmmunology.

    • Related Report
      1990 Annual Research Report
  • [Publications] Nishimura,Y.: "A possible association between HLA and HYTLVーI associated myelopathy in Japanese" Tissue Antigens.

    • Related Report
      1990 Annual Research Report
  • [Publications] 土屋 邦善: "DNA解析による疾患感受性の検出(RAとSLE)" 内科. 66. 697-698 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 土屋 邦善: "分子遺伝学:RA感受性を支配するDRB1遺伝子" 病理と臨床. 8. 1372-1375 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 土屋 邦善: "HLAタイピング" 現代医療. 23. 159-168 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] 西村 泰治: "HLAーDQトランスジェニックマウス" 代謝26巻増刊号「免疫'89」. 26. 159-168 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] 西村 泰治: "免疫抑制とMHC" 臨床免疫. 21. 1362-1372 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] 西村 泰治: "ヒトの免疫抑制遺伝子" 医学のあゆみ. 151. 582-585 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] 西村 泰治: "HLAーDQと免疫抑制" Annual Review免疫1990. 463-466 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 西村 泰治: "HLAーDQトランスジェニックマウス" 医学のあゆみ. 154. 482 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 西村 泰治: "免疫応答および疾患感受性を支配する遺伝要因" 日本体質学会雑誌. 54. 45-47 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 西村 泰治: "ヒト免疫応答の個体差とその遺伝" アレルギ-の臨床. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 西村 泰治: "RFLP解析を用いたHLAクラスII遺伝子のタイピング" Clinical Immunology Newsletter. 10. 60 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 岡本 安弘: "慢性関節リウマチの理解のために,5遺伝「慢性関節リウマチ」水島裕編集" 南江堂 東京, 237-242 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 西村 泰治: "抗原特異的T細胞増殖反応による免疫抑制遺伝子の解析「生化学実験講座」第12巻分子免疫学II免疫遺伝学・アレルギ-、日本生化学会編" 東京化学同人 東京, (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 西村 泰治: "トランスジェニックマウスを用いたMHCの解析「生化学実験講座」第12巻分子免疫学II免疫遺伝学・アレルギ-、日本生化学会" 東京化学同人 東京, (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Y.Nishimura: "Expression of human major histocompatibility complex,HLA-DQw6 genes alters the immune response in C57BL/6 mice."

    • Related Report
      1989 Annual Research Report
  • [Publications] Y.Fukui: "Glycosuria and insulitis in NOD mice expressing the HLA-DQw6 molecule." Journal of Immunogenetics.

    • Related Report
      1989 Annual Research Report
  • [Publications] K.Fujisawa: "Differential contribution of the HLA-DR and DQ molecules in the autologous and allogeneic mixed lymphocyte reactions."

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Sasazuki: "“Expression and function of human major histocompatibility complex HLA-DQw6 genes in the C57BL/6 mouse"in Cold Spring Harbor symposium on Quntitative Biology,vol 54" Cold Spring Harbor Laboratory, 10 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Sasazuki: "“Differential roles of HLA-DR and DQ in immune regulation"in Progress in Immunology VII" Springer Verlag,Berlin, 7 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Nishimura,Y.;Bierer,B.E.;Burakoff,S.J.: J.Immunology. 141. 3438-3444 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Nishimura,Y.;Bierer,B.E.,Jones;Jones, W.K.;Jones,N.H.;Strominger,J.L.;Burakoff,S.J.: T cells.Eur.J.Immunol. 18. 747-753 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Bierer,B.E.;Nishimura,Y.;Burakoff,S.J.;Smith,B.F.: J.Clin,Invest.81. 1390-1397 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Sasazuki,T.;Kikuchi,I.;Hirayama,K.;Matsushita,S.;Ohta,N.;Nishimura,Y.: Immunology. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 西村泰治: "代謝病ハイライト CD(T3)複合体" 中山書店, 385-389 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 西村泰治: "Medical Immunology 第16巻 ヒト細胞における付着分子" 国際医書出版, 561-568 (1988)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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