| Project/Area Number |
63480234
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Pediatrics
|
|---|
| Research Institution | Tokyo Medical and Dental University |
|---|
Principal Investigator |
YATA Junichi Tokyo Medical and Dental University Professor Faculty of Medicine, 医学部, 教授 (60057502)
|
|---|
| Project Period (FY) |
1988 – 1990
|
| Project Status |
Completed (Fiscal Year 1990)
|
| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1990: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1989: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1988: ¥3,300,000 (Direct Cost: ¥3,300,000)
|
|---|
| Keywords | Primary immunodeficiency / T cell differentiation / Regulation by NK cells / sigmadelta T cells / Allergen-specific B cells / Hashimot's thyroiditis / Anti-thyroglobulin antibody / キラ-細胞 / インタ-ロイキン / γδ型T細胞 / 抗原結合B細胞 / 卵白アレルギ- / σδ型T細胞 / 細胞傷害活性 / T細胞レセプタ- / インタ-ロイキン2 / 免疫グロブリン産生不全 / 抗原特異的β細胞 / NK細胞 / T細胞レセプター / キラーT細胞 / 抗原結合リンパ球 / 免疫調節 / 自己免疫病 / アレルギー |
|---|
| Research Abstract |
The lymphocytes from immune disorders were analyzed for their differentiation and the regulatin of their functions in order to disclose the pathogenesis. T cells can be divided into alphabeta type and sigmadelta type according to the peptides constituting the antigen receptor. The later T cells are less dependent on the thymus and seem to be more primitive. Sigmadelta T cells were increased in some of the immunodeficiency patients with T cell deficiency indicating compensations of ordinary T cells. However, part of the cytotoxic function of these sigmadelta T cells was impaired and their differentiation was suggested to be not complete. Bone marrow cells and later irradiated thymus were transplanted to a patient with T cell defect probab lly due to impaired development of lymphoid stem cells. NK cells was generated but T cells developed only after thymus transplantation. NK cells seemedo down-regulate the generation of granulocytes and T cells did that of NK cells, which suggested the
… More
presence of mutual regulation among granulocytes, NK cells and T cells. Thus appeared sigmadelta T cells responded well to interleukin (IL) -2 but alphadelta T cells did not. Allogeneic irradiated thymus might not be sufficient for the full differentiation of alphadelta T cells. It was suggested by a case with normal B cells and hypogammaglobulinemia that impaired differentiation of CD4^+T cells from CD45RA^+ to CD45RO^+ causes defective helper function. The number of ovalbumin (OVA) specific B cells was observed to increase gradually according to age and was more prominent in OVA-allergic patients. The increase of OVA-specific B cells preceded to the appearance of serum anti-OVA antibody. Enumulation of allergenspecific B cells may be valuable to predict food allevgy. In vitro study of antithyroglobulin antibody production from Hashimoto's thyroiditis revealed that B cells were fully activated in some patients and they were inactive for moment but showed potentiality to produce the antibody in other patients. Less
|
