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Studies on Mechanism of Proliferation of Lymphocytis to Produce Platelet Associated Immunoglobulin.

Research Project

Project/Area Number 63480240
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Pediatrics
Research InstitutionTokyo Jikei University School of Medicine Department of Pediatrics

Principal Investigator

AKATSUKA Jun-ichi  Professor, Department of Pediatrics, Tokyo Jikei Univ. School of Medicine, 医学部・小児科学教室, 教授 (20056550)

河野 通雄 (1989)  神戸大学, 医学部, 教授 (60030938)

Co-Investigator(Kenkyū-buntansha) IKEGAMI Mayumi  Research Assistant, Department of Pediarrics, Tokyo Jikei Univ. School of Medici, 小児科学教室, 助手 (40184410)
KOBAYASHI Naoaki  Research Assistant, Department of Pediatrics, Tokyo Jikei Univ. School of Medici, 小児科学教室, 助手 (50186756)
UCHIYAMA Hiroshi  Research Assistant, Department of Pediatrics, Tokyo Jikei Univ. School of Medici, 小児科学教室, 助手 (10168718)
ISHIDOYA Naoko  Research Assistant, Department of Pediatrics, Tokyo Jikei Univ. School of Medici, 小児科学教室, 助手 (80168244)
FUJISAWA Koji  Research Assistant, Department of Pediatrics, Tokyo Jikei Univ. School of Medici, 小児科学教室, 助手 (10130197)
松井 律夫  神戸大学, 医学部, 助手 (60199742)
土師 守  神戸大学, 医学部附属病院, 助手 (10198707)
廣田 省三  神戸大学, 医学部附属病院, 講師 (20181216)
佐古 正雄  神戸大学, 医学部附属病院, 助教授 (60030970)
Project Period (FY) 1988 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 1990: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1989: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1988: ¥3,400,000 (Direct Cost: ¥3,400,000)
KeywordsIDIOPATHIC THROMBOCYTOPENIC PURPURA / PAIGG / ELIZA-PLAQUE ASSAY / LAIGG / ANTIGEN COMBINE WITH AUTOIMMUNE ANTIBODY / GPIIb / IIIa / 特発性血小板減少性紫斑病 / PBIgG / 血小板抗体対応抗原 / 肝癌 / 肝細胞癌 / 集団検診 / 超音波検査 / X線CT / MRI / 画像診断 / flowcytometry / モノクロナ-ル抗体 / ELIZA-plaqueassay / エピト-プ / PAIgM / PAC3 / ELISA-Plaque法 / PAIgG産生リンパ球
Research Abstract

1) Mechanism of proliferation of PAIgG-secreting lymphocytes.
We developed the new modified methods of ELIZA-plaque assay which could identify and count the lymphocytes to secrete platelet associated IgG (PAIgG) in mouse spleen cells sensitized with human platelets. The proliferation of PAIgG secreting spleen cells of mouse were suppressed with such drugs as cyclophosphamide, bolus prednisolone and prednisolone of long period. However, we could not detect PAIgG-secreting lymphocytes in spleen cells of children with chronic ITP and the peripheral lymphocytes of the patient sensitized with isologous platelets transfusion.
On the other hand, we immunized mouse with IgG fraction of the children whose sera contained platelet bound IgG and produced the polyclonal antibody for antihuman IgG. Using this antibody, we could found incresed number of the lymphocytes combined with IgG (LAIgG) in the peripheral blood of children with chronic ITP, Evans syndrome and ulcerative colitis. Then, the lymphocytes with LAIgG were shown to be T-cells. The student are going to make clear the significance of the increase of lymphocytes with LAIgG in those children.
2) Detection of antigen site on the palatelet membrane combined with autoantibody.
We measured the amount of monoclonal antibodies to conbine with platelet membrane as for platelets of children with chronic ITP, Evans syndrome and thromboasthenia. Five of 22 children with chronic ITP showed decreased amount of monoclonal antibodies combined with platelet membrane. This phenomenon suggested that there were the antigen site to combine with autoimmune platelet antibodies near the epitope of GPIIb/IIIa.

Report

(5 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (57 results)

All Other

All Publications (57 results)

  • [Publications] 赤塚 順一 他: "小児ITP患児の発育障害および残存異常、" 厚生省特定疾患、特発性造血障害調査研究班、昭和62年度業績報告、. 248-249 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 赤塚 順一 他: "小児ITP患児の血小板結合免疫グロブリン及び補体の検討" 厚生省特定疾患、特発性造血障害調査研究班、昭和62年度業績報告、. 250-251 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 内田 浩志 他: "ELIAAーplaque assayによる血小板抗体産生脾細胞の検出、" 医学のあゆみ. 144. 823-824 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 藤沢 康司 他: "小児急性及び慢性ITPに対するメチルプレドニソロン療法と免疫グロブリン大量療法の比較検討、" 日児誌、. 92. 91-97 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 赤塚 順一: "PAIgGの測定とその臨床的意義、" 日小血会誌、. 2. 1-8 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 赤塚 順一: ":血小板と免疫、Currents in Hematoimmunology," Excerpta Medica,. 4. 3-8 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 赤塚 順一: "特発性血小板減少性紫斑病(小児)" ,Medicina,. 25. 2330-2331 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 藤沢 康司: "血小板機能異常症" 小児内科. 20. 995-1000 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Akatsuka J,et al.: "Longーterm followーup studies of children of children with chronic ITP." Blut. 59. 105-108 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 安永 幸二郎 他: "完全分子型液状免疫グロブリン(Cー425)大量投与による特発性血小板減少性紫斑病の治療成績、" 基礎と臨床. 23. 3269-3279 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Uciyama H.et al.: "ELIZAーplaque assay for detection of platelet antibody secreting cells.I.Studies on optimization of reagent concentrations and culture conditions for enumeration of spotーforming cells." J of Immunological Methods,. 117. 199-203 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 赤塚 順一: ":種々の疾患における脾摘の適応とその遠隔成績" 小児外科. 21. 131-135 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Uchiyama H,et al.: "ELIZAーplaque assay for the detection of platelet antibodyーsecreting cellsーEffects of various immunosuppressive treatment on platelet antibodysecreting cells." Jikeikai Med J.37. 407-413 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 藤沢 康司 他: "全血flow cytometryによる慢性ITPにおける自己抗体対応抗原の検討、" 臨床血液、. 31. 1769-1773 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 赤塚 順一: "免疫グロブリン療法の最近の知見、特発性血小板減少性紫斑病、" 小児医学、. 23. 673-691 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 池上 真由美、藤沢 康司、赤塚 順一: "小児特発性血小板減少性紫斑病における血小板結合免疫グロブリンに関する研究第1編、フロ-サイトメトリ-による血小板結合グロブリンの検出と血小板形態解折、" 日児誌、. 92. 1866-1873 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 池上 真由美、藤沢 康司、赤塚 順一: "小児特発性血小板減少性紫斑病における血小板結合免疫グロブリンに関する研究、第2編、血清中の血小板結合性IgGの同種血小板への作用について、" 日児誌、. 94. 2135-2159 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 赤塚 順一: ":小児の血小板と免疫、血小板1988ー血小板と免疫ー 蔵本 淳、青木 信雄、大熊 稔、日高 弘義、 山崎 博男編" 、科学評論社, 117-129, (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 赤塚 順一: ":小児特発性血小板減少性紫斑病の特徴と治療、小児科Mook,馬場 一雄、小林 登、山田 兼雄、" 金原出版, 119-128 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 赤塚 順一: ":血液疾患に対する免疫グロブリン療法、新小児医学体系、小児医学の進歩、" 153-167 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Fujisawa K, Akatsuka J. et al.: "Comparison of bolus methylprednisolone therapy and high-dose immunoglobulin intravenous infusion of children with acute and chronic ITP" J of Jap Pediatr Soc. 92. 91-97 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Akatsuka J.: "Clinical significance of measurement of platelet associated IgG." Jap J of Pediatr Hematol. 2. 1-8 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Akatsuka J.: "Platelet and immunology" Excerp Medica. 4. 3-8 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Akatsuka J.: "Characteristics treatment of children with idiopathic thrombo-cytopenic purpura" Pediatric book. 54. 119-128 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Fujisawa K, Akatsuka J.: "Thrombocytopathy" Jap J of Pediatr Med. 20. 995-1000 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ikegami M, Fujisawa K, Akatsuka J.: "Platelet associated immunoglobulin in idiopathic thrombocytopenic purpura in childhood. Part. 1. Analysis of platelet associated immunoglobulins and platelet volume by flow cytometry." J of Jap Pediatr Soc. 92. 1866-1873 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Akatsuka J, Fujisawa K, et al.: "Long-term follow-up studies of children with chronic idiopathic thrombocytopenic purpura" Blut. 59. 105-108 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Uchiyama H, Akatsuka J.: "ELIZA-plaque assay for the detection of platelet antibody-secreting cells. 1. Studies on optimization of reagent concentrations and culture condition for enumeration of spot-forming cells" J Immunol Methods. 117. 199-203 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Akatsuka J.: "Indication and prognosis of splenectomy of various kinds of diseases in children" Jap J of Pediatr Surgery. 21. 131-135 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Akatsuka J.: "Immunoglobulin therapy of children with hematologic diseases" Shinshoniigakutaikei, Nakayama Publisher, Tokyo. 153-167 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Uchiyama H, Akatsuka J.: "ELIZA-plaque assay for the detection of platelet antibody-secreting cells. Effects of various immunosuppressive treatment on platelet antibody-secreting cells" Jikei Med J. 37. 407-413 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Fujisawa K, Akatsuka. et al.: "Detection of antigenic determinations of autoantibodies in idiopathic thrombocytopenic purpura using anti-platelet monoclonal antibodies and flow cytometry in whole blood" Rinshoketsueki. 31. 1769-1773 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Akatsuka J.: "Recent review of immunoglobulin therapy. -Idiopathic thrombocytopenic thrombocytopenic purpura-" Pediatric Review. 23. 673-691 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ikegami M, Fujisawa K, Akatsuka J.: "Platelet associated immunoglobulin in idiopathic thrombocytopenic purpura in childhood. Part 2. The effect of anti-platelet IgG antibody on platelet binding proteins and its morphology."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Uchiyama H,Akatsuka J: "ELIZAーplaque assay for the detection of platelet antibodyーsecreting cellsーEffects of various immunosuppressive treatment on platelet antibodyーsecreting cells." Jikeikai Med J.37. 407-413 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 藤沢 康司、池上 真由美、赤塚 順一:"全血flow cytometryによる慢性ITPにおける自己抗体対応抗原の検討." 臨床血液、. 31. 1769-1773 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 内山 浩志、赤塚 順一、: "小児再生不良性貧血及びITP患児の慢性移行に関する検討、の慢性移行に関する検討、厚生省特定疾患、特発性造血障害調査研究班、"

    • Related Report
      1990 Annual Research Report
  • [Publications] 内山 浩志、赤塚 順一、広津 卓夫、星 順隆: "小児再生不良性貧血及びITP患児の慢性移行に関する検討、第52回日本血液学会、3,29,1990,東京"

    • Related Report
      1990 Annual Research Report
  • [Publications] 池上 真由美、藤沢 康司、赤塚 順一: "小児特発性血小板減少性紫斑病における血小板結合免疫グロブリンに関する研究、第2編、血清中の血小板結合性IgGの同種血小板への作用について、" 日児誌、. 94. 2135-2159 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 小林 尚明、赤塚 順一,他:"ITP患者におけるIgG結合リンパ球の臨床的意義について、第32回日本臨床血液学会総会、9,26,1990 東京"

    • Related Report
      1990 Annual Research Report
  • [Publications] 赤塚 順一: "免疫グロブリン療法の最近の知見、特発性血小板減少性紫斑病、" 小児医学、23:673ー691,990(医学書院), 18 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 渡辺英明,佐古正雄,廣田省三: "Dynamic CTによる肝血流の評価-実験的並びに臨床的研究" 日本医学放射線学会雑誌. 48. 972-981 (1988)

    • Related Report
      1989 Final Research Report Summary
  • [Publications] 河野通雄,廣田省三,佐古正雄: "肝臓がんの集検に関する研究-肝スクリ-ニングのための画像診断法の研究" Innervision. 4. 15-16 (1989)

    • Related Report
      1989 Final Research Report Summary
  • [Publications] 上田英二,廣田省三,藤井正彦,田中浩司,土師守,清水雅史,佐古正雄,河野通雄: "高度の脂肪沈着を伴った肝細胞癌の3例" 臨床放射線. 34. 921-924 (1989)

    • Related Report
      1989 Final Research Report Summary
  • [Publications] 河野通雄,佐古正雄,廣田省三,土師守: "腹部臓器の画像診断" 日本臨床外科医学会雑誌(掲載予定).

    • Related Report
      1989 Final Research Report Summary
  • [Publications] Akatsuka,J.et al.: "Long-term follow up study of children with chronic ITP." Blut. 59. 105-108 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Uchiyama,H.,et al.: "ELISA-plaqueassay for the detection of platelet antibody-screening cells." J.of Immunolgical Mehod. 117. 199-203 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 内山浩志他: "先天性赤芽球疲患児の諸種治療における造血能及び免疫学的パラメ-タ-の検討" 日本血液学会雑誌. 52. 588-593 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 赤塚順一: "種々の疾患における脾摘の適応と遠隔成績" 小児外科. 21. 131-135 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 池上真由美: "ITP患児血清IgG分画の抗血小板作用について" 日本小児科学会雑誌.

    • Related Report
      1989 Annual Research Report
  • [Publications] 赤塚順一: "血液疾患に対する免疫グロブリン大量療法" 新小児科学的体系,小児医学の進歩‘89B,中山書店,東京, 153-167 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 赤塚順一: 日本小児血液学会誌. 2. 1-8 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 赤塚順一: Currents in Hemafoimmunology. 4. 3-8 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 赤塚順一: 血小板1988. 117-129 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 赤塚順一: 小児科Mook. 54. 119-128 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 藤沢康司 他: 小児内科. 20. 995-1000 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 池上真由美 他: 日本小児科学会誌. 92. 1866-1873 (1988)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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