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Effect of Aging on the Structure and Sebum of Human Sebaceous Glands ; Morphological and Biochemical Study

Research Project

Project/Area Number 63480244
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Dermatology
Research InstitutionNiigata University

Principal Investigator

SATO Yoshio  Niigata University School of Medicine, Department of Dermatology, Professor, 医学部, 教授 (80018291)

Co-Investigator(Kenkyū-buntansha) ITO Masaaki  Niigata University School of Medicine, Associate Professor, 医学部, 助教授 (30115000)
Project Period (FY) 1988 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1990: ¥100,000 (Direct Cost: ¥100,000)
Fiscal Year 1989: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1988: ¥2,700,000 (Direct Cost: ¥2,700,000)
KeywordsAging / Sebaceous Gland / Computer-Stereography / Electron Microscopy / Sebum / Lipid Analysis / Urinary Androgen / ヒト脂腺形態 / 尿中17-KS / 薄層クロマトグラフィ- / ガスクロマトグラフィ- / 脂腺活動性 / 年令推移 / 薄層クロマトグラフィー / ガスクロマトグラフィー / 年齢推移
Research Abstract

To elucidate the changes of human sebaceous gland function with aging, forehead sebaceous glands of normal individuals of various ages were examined by computer-stereography and electron microscopy, and their forehead sebum samples were biochemically analyzed. The three-dimensional analysis of sebaceous glands using a computer graphic analysis system (Cosmozone 2SA, Nikon, Tokyo) was performed. In females, the volumes of sebaceous glands were small in subjects of less than 10 years of age, the largest at 20s and became smaller after 50s. In males, although they also reached the maximum at 20s,
they maintained the large sizes until 40s or 50s and began to decrease at 60s. The larger average volumes of sebaceous glands showed the larger standard deviation values. The large sebaceous glands stereographically revealed balloon-like swelling. The increase in volume of sebaceous gland was due to increases in both number and size of sebocytes. Ultrastructurally, in the large sebaceous glands of … More young adults the peripheral sebocytes were cuboid in shape and the mature sebocytes had a large number of lipid droplets of a similar size, whereas in the small ones of children and aged persons the peripheral sebocytes were flat in shape and the mature sebocytes had lipid droplets of various sizes. These morphological and morphometrical data suggest that there are distinct age-related structural changes of human sebaceous glands in both sexes, although there is a difference in their size between the sexes in middle-aged individuals. Sebum samples were obtained from the forehead skin of normal human individuals of various ages by an absorption method. The amount of sebum rapidly increased until 20s in both sexes. It gradually decreased after 40s in females, whereas it was maintained at a high level after 40s in males. Wax esters/[cholesterol + cholesterol esters] (WE/[C+CE]) rations, which may be a good index to express the sebaceous gland activities, in the sebum samples, were measured by thin-layer chromatography. The age-related change of the ratios was similar to that of sebum amounts in females, but it was not in males. The ratios in males decreased after 40s similarly to those in females, although they kept higher levels in males than females. The WE fractions were extracted from the sebum samples and their fatty acid methyl esters were analyzed by gas-liquid chromatography using fused-silica capillary column. The percentages of C_<16:1> in WE also showed age-related changes, corresponding to those of WE/[C+CE] ratios, in both sexes. Urinary androgens were separated from 24 hrs-urine samples of all the individuals, trimethylsilylated and submitted to analysis by gas-liquid chromatography. The amounts of testosterone showed age-related changes positively correlated with those of either WE/[C+CE] ratios or C_<16:1> straight chain fatty acid proportions in sebum in both sexes. The amount of 17-ketosteroid also revealed a positive correlation with these sebum indices in females, but in males it did not show such a correlation except for infancy period. The activity of male sebaceous glands seemed to be controlled principally by testosterone as an intrinsic factor, whereas the female sebaceous glands seemed to be more sensitive to adrenal androgens than male ones. These morphological and biochemical findings may indicate that human sebaceous glands show distinct age-related changes of structures and functions in both sexes, although there are some differences in the patterns of the changes between the sexes especially in middle ages. Less

Report

(4 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Yamamoto,A.,Serizawa,S.,Ito,M.and Sato,Y.: "Effect of aging on sebaceous gland activity and on the fatty acid composition of wax esters." The J.of Investigative Dermatology. 89. 507-512 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 増子 倫樹: "女性における脂腺の年齢による形態変化" 日本皮膚科学会雑誌. 98. 443-452 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yamamoto,A.,Serizawa,S.,Ito,M.and Sato,Y.: "Fatty acid composition of sebum wax esters and urinary anarogen leve in normal human individuals" J.of Dermatological Science. 1. 269-276 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yamamoto,A.,Serizawa,S.,Ito,M.and Sato,Y.: "Characterization of human sebaceous gland activity with age" Acta Medica et Biologica. 38. 159-166 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 池田 和人: "男貞における脂腺の年齢における形態的変化" 日本皮膚科学会雑誌. 101.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 佐藤 良夫, 伊藤 雅章, 山本 綾子, 芹澤 重男, 増子 倫樹, 横山 博之: "「加齢と皮膚」皮脂" 清至書院, 16 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yamamoto,A.,Serizawa,S.and Sato,Y.: "Cutaneous Aging" University of Tokyo Press, 10 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yamamoto, A., Serizawa, S., Ito, M. and Sato, Y.: "Effect of aging on sebaceous gland activity and on the fatty acid composition of wax esters" The J. of Investigative Dermatology,. 89 (5). 507-512 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Mashiko, T.: "Morphological changes in sebaceous glands with aging in females : microcomputer graphic analysis and electron microscopic investigation" The Jpn. J. of Dermatology,. 98 (4). 443-452 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yamamoto, A., Serizawa, S. and Sato, Y.: "Sebum secretion and urinary androgen level" Cutaneous Aging, ed. by Kligman, A. M., Takase, Y., University of Tokyo Press, Tokyo,. 149-158 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yamamoto, A., Serizawa, S., Ito, M. and Sato, Y.: "Fatty acid composition of sebum wax esters and urinary androgen level in normal human individuals" J. of Dermatological Science,. 1 (4). 269-276 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yamamoto, A., Serizawa, S., Ito, M. and Sato, Y.: "Characterization of human sebaceous gland activity with age" Acta Medica et Biologica,. 38 (4). 159-166 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ikeda, K.: "Morphological changes in sebaceous glands with aging in males" The Jpn. J. of Dermatology,. 101 (3).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yamamoto,A.,Sarizawa,S.,Ito,M.and Sato,Y.: "Effect of aging on sebaceous gland activity and on the fatty acid composition of wax esters." The J.of Investigative Dermatology. 89. 507-512 (1987)

    • Related Report
      1990 Annual Research Report
  • [Publications] 増子 倫樹: "女性における脂腺の年齢による形態変化" 日本皮膚科学会雑誌. 98. 443-452 (1988)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yamamoto,A.,Sarizawa,S.,Ito,M.and Sato,Y.: "Fatty acid composition of sebum wax esters and urinary androgen level in normal human individuals." J.of Dermatological Science. 1. 269-276 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yamamoto,A.,Sarizawa,S.,Ito,M.and Sato,Y.: "Characterization of human sebaceous gland activity with age." Acta Medica et Biologica. 38. 159-166 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 池田 和人: "男性における脂腺の年齢による形態的変化" 日本皮膚科学会雑誌. 101.

    • Related Report
      1990 Annual Research Report
  • [Publications] 佐藤 良夫,伊藤 雅章,山本 綾子,芹澤 重男,増子 倫樹,横山 博之: "「加齢と皮膚」皮脂" 清至書院, 16 (1986)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yamamoto,A.,Serizawa,S.and Sato,Y: "Cutaneous Aging" University of Tokyo Press, 10 (1988)

    • Related Report
      1990 Annual Research Report
  • [Publications] Ayako Yamamoto,他: "Effect of aging on sebaceous gland activity and on the fatty acid composition of wax esters." J.Invest.Dermatol.89. 507-512 (1987)

    • Related Report
      1989 Annual Research Report
  • [Publications] Ayako Yamamoto,他: "Fatty acid compositions of sebum wax esters and urinary androgen level in normal human individuals." J.Dermatol.Sci.

    • Related Report
      1989 Annual Research Report
  • [Publications] 佐藤良夫,他: "加齢と皮膚" 清至書院, 16 (1986)

    • Related Report
      1989 Annual Research Report
  • [Publications] Ayako Yamamoto,他: "Cutaneous Aging" University of Tokyo Press, 9 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] 伊藤雅章: "皮膚付属器疾患" 日本皮膚科学会専門医委員会, 20 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Ayako,Yamamoto 他: J.Invest.Dermatol.89. 507-512 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] 佐藤良夫 他: "加齢と皮膚" 清至書院, 91-106 (1986)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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