| Project/Area Number |
63480246
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Dermatology
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| Research Institution | Kitasato University School of Medicine |
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Principal Investigator |
NISHIOKA Kiyoshi Kitasato Univ. Sch. of Med. Associate Prof., 医学部, 助教授 (20077647)
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| Co-Investigator(Kenkyū-buntansha) |
HIGASHI Kazunori Kitasato Univ. Sch. of Med. Lecturer, 医学部, 助手 (00189747)
YOKOZEKI Hiroo Kitasato Univ. Sch. of Med. Lecturer, 医学部, 助手 (90210608)
ETO Hikaru Kitasato Univ. Sch. of Med. Assistant Prof., 医学部, 講師 (20137920)
KATAYAMA Ichiro Kitasato Univ. Sch. of Med. Assistant Prof., 医学部, 講師 (80191980)
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| Project Period (FY) |
1988 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 1989: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1988: ¥3,200,000 (Direct Cost: ¥3,200,000)
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| Keywords | Epidermal cytokine / Psoriasis / Interleukin 1 / Interleukin 6 / In situ hybridization / 尋常性乾癬 / 表皮サイトカイン / 乾癬 / IL1 / IL6 / インサイチュ・ハイブリダイゼ-ション / 表皮細胞株 / 表皮細胞由来胸腺細胞活性化因子(ETAF) / インターロイキン1α |
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| Research Abstract |
We have developed a method to detect messenger RNA (mRNA) expression of keratinocytes in situ in the study performed in the previous year. We have applied this technique to examine the role of interleukins in psoriasis, one of typical epidermal proliferating diseases, this year.
Biopsied specimens were obtained from 27 patients with psoriasis vulgaris and 9 healthy volunteers after informed consent. They were snap-frozen in liquid nitrogen and 10 mu thick sections were prepared. The sections were fixed with 4% paraformaldehyde and ethanol, and hybridized with biotin labeled complementary DNA (cDNA) of interleukin (IL) -1, IL-6 and IL-6 receptor (IL-6R). They were treated with avidin alkaline phosphatase and its enzyme substrates.
IL-1 message was expressed by healthy skin, uninvolved skin of psoriasis and transitional zone between uninvolved and fully developed psoriatic lesion but not in psoriatic lesion. The message of IL-6 was detected in the uninvolved, transitional zone and psoriatic lesion but not in healthy skin. It was expressed more strongly in transitional zone and tended to fade in psoriatic lesion. IL-6R message was found in uninvolved and transitional zone but not in psoriatic lesion and healthy skin.
Thus synthesis of IL-6 and its receptor was regulated in the psoriatic skin by certain factors to suppress excess proliferation as is found in neoplasmas. IL-6 and its receptor expression by psoriatic skin play an important role in pathogenesis of psoriasis.
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