| Project/Area Number |
63480277
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Hematology
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
YOSHIDA Yataro (1989) Assist. Prof., lst Div., Dept. Med., 医学部, 講師 (80064525)
内野 治人 (1988) 京都大学, 医学部, 教授 (40034615)
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| Co-Investigator(Kenkyū-buntansha) |
UCHINO Haruto , 名誉教授 (40034615)
OGUMA Shigeru Assist., lst Div., Dept. Med., 医学部, 助手 (00169260)
SASADA Masataka Prof., College of Med. Technol., 教授 (30144364)
YAMAGISHI Morihisa Prof., Health Care Inst., Shiga Univ., 保健管理センター, 講師 (50026829)
ITOH Kazuhiko Assist. Prof., Blood Transf. of Service, 医学部, 講師 (50034640)
吉田 弥太郎 京都大学, 医学部, 講師 (80064525)
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| Project Period (FY) |
1988 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1989: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1988: ¥5,400,000 (Direct Cost: ¥5,400,000)
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| Keywords | MDS / preleukemia / hematopoietic stem cells / cytokines / differentiation / MDS derived inhibition of CFU-gm / ineffective hematopoiesis / cellular dysfunction / 血球の分化異常と分化誘導 |
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| Research Abstract |
A. Hematopoietic abnormalities in the myelodysplastic syndrome: Initial CFU-gm growth was significantly related to the survival. In addition, sequential CFU-gm growth patterns correlated with different clinical outcome(leukemic transformation, non-leukemic death and stable disease).
B. CFU-gm inhibitory macrophages in MDS marrow: When nonadherent marrow mononuclear cells were expanded in liquid culture by GM-CSF, a population of cells was obtained which were predominantly macrophages and inhibited the growth of normal marrow CFU-gm. The inhibition was mediated by soluble factors produced by these inhibitory macrophages.
C. Establishment of a novel cell line MYU-1 from MDS marrow: A new cell line MYU-1 was established from the marrow cells of a patient with CMMOL. MYU-1 cells have the unique capacity to proliferate in the presence of marrow stroma and undergo monocytoid and eosinophilic differentiation.
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