| Project/Area Number |
63480288
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Mie University |
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Principal Investigator |
IRIYAMA Keiji Mie University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (10024754)
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| Co-Investigator(Kenkyū-buntansha) |
HONZUMI Makoto Mie University, Faculty of Medicine, Assistant Professor, 医学部附属病院, 講師 (30144265)
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| Project Period (FY) |
1988 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 1989: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1988: ¥5,900,000 (Direct Cost: ¥5,900,000)
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| Keywords | Fat Emulsion / Parenteral Nutrition / Apolipoproteins / Lipoproteins |
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| Research Abstract |
Artificial lipid particles used as parenteral nutrition solution do not contain any apolipoproteins when they are infused into the circulation.
Despite the absence of apolipoproteins, the metabolism of artificial lipid particles is similar to that of chylomicrons which contain various kinds of apolipoproteins. Of the known apolipoproteins, apolipoproteins C-II and C-III are important in regulation of hydrolysis of triglycreride-rich lipoproteins. Modifications of apolipoproteins associated with an intravenous infusion of fat emulsion were investigated, in vivo and in vitro. Artificial lipid particles acquired apolipoproteins C-II and C-III from high-density lipoprotein immediately after their entry into plasma. The transfer of apolipoproteins to artificial lipid particles was not influenced by a prior injection of fat emulsion. When the concentrations of triglycerides in plasma reached a plateau after initiation of a continuous infusion of fat emulsion, the distribution of C apolipoproteins in the lipoprotein fraction also stabilized. Since C apolipoproteins moved to artificial lipid particles from high-density lipoprotein even in vitro, it has become evident that artificial lipid particles have a close affinity for C apolipoproteins, and that no specific organ is responsible for the transfer of C apolipoproteins between artificial lipid particles and plasma lipoproteins.
Apolipoprotein E can not be transferred from plasma lipoproteins to artificial lipid particles simply as the result of an affinity of artificial lipid particles for apolipoprotein E.
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