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Vaccination Therapy For Malignant Tumor with Antiidiotypic Monoclonal Antibodies

Research Project

Project/Area Number 63480295
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General surgery
Research InstitutionTokyo Medical University

Principal Investigator

KUSAMA Mikihiro  Tokyo Medical College, School of Medicine, Assistant Professor, 医学部・外科, 助手 (90201467)

Co-Investigator(Kenkyū-buntansha) KAWASHIMA Takeshi  Tokyo Medical College, School of Medicine, Assistant Professor, 医学部・皮膚科, 講師 (00175291)
Project Period (FY) 1988 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥5,100,000 (Direct Cost: ¥5,100,000)
Fiscal Year 1990: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1989: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1988: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsAntiidiotybic Antibody / Monoclonal Antibody / Cancer Vaccination / Immunotherapy / Tumor Immunity / Epitope / Melanoma Associated Antigen / Tumor Associated Antigen / 腫瘍関連抗原 / モノクローナル抗体 / ヒト高分子メラノーマ関連抗原 / エピトープ
Research Abstract

Monoclonal antibody C97 which recognized the antigen that expressed on the tumor cells of colon and pancreatic carcinoma with high incidence and AFDO46 which recognized the alpha protein antigen that expressed on the tumor cells of primary hepatic carcinoma were immunized to mice, and antiidiotypic monoclonal antibodies were prepared with hybridization. These anti Id MoAbs reacted only with their immunized MoAbs strongly, but not reacted with other MoAbs. And the reaction of these anti Id MoAbs were very specific. Furthermore, these anti Id MoAbs inhibited the binding intensity of the MoAbs to tumor cells strongly. These results showed they recognized their private epitope. But, the activity of anti C97 Id MoAbs were so weak that we lost the activity finally. Three of anti AFDO46 Id MoAbs were established. By inhibition test, these IgG_1 MoAbs recognized the very similar part of epitope. Now we were investigating whether these anti Id MoAbs had the internal image or not, and we were investigating the effect to grow a transplanted AFP secreting tumor in animal model. Anti HMW-MAA MoAb MK2-23 which established by head investigator of this project were found that this MoAb had the internal image of HMW-MAA. This result suggested this MoAb had the ability of induction for human immunoglobulin. The result of clinical trial for anti Id MoAb were 4 cases of NC and 2 cases of ND. This new aspect expected the clinical results got better. Two of new aspects were gained in this projects. The first one was that a MoAb which cross-reacted with an antigen on the guinea pig were able to analyze with anti Id MoAbs. The other aspect was established the classification of many anti HMW-MAA MoAbs.

Report

(4 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] 日馬 幹弘: "抗腫瘍モノクロ-ナル抗体に対する抗イディオタイプモノクロ-ナル抗体の作製を臨床応用" Biotherapy. 3. 329-333 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Mikihiro Kusama: "Characterization of Syngeneic Antiidiotypic Monoclonal Antibodies to Murine AntiーHuman High Molecular Weight MelanomaーAssociated Antigen Monoclonal Antibodies" The Journal of Immunology. 143. 3844-3852 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 日馬 幹弘: "モルモット腫瘍上の抗原と交又反応を呈したヒト腫瘍関連抗原の解析" 医学のあゆみ.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Mikihiro Kusama: "Analysis with Monoclonal Antibodies of the Serological Heterogeneity of Human High Molecular Weight Melanoma Associated Antigen" Gann.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 日馬 幹弘: "抗ヒトAFPモノクロ-ナル抗体に対する同系抗イディオタイプモノクロ-ナル抗体"

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Mikihiro kusama: "Antiidiotypic Monoclonal Antibodies to Murine Anti AFP Monoclonal Antibodies" Cancer Immunol.Immunother.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Mikihiro Kusama et al.: "Production and clinical application of antiidiotypic monoclonal antibody for anti-tumor monoclonal antibodies." BIOTHERAPY. 3(1). 329-333 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Mikihiro Kusama et al.: "Characterization of syngeneic antiidiotypic monoclonal antibodies to murine anti-human high molecular weight melanoma-associated antigen monoclonal antibodies." J. of Immunology. 143(11). 3844-3852 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Mikihiro Kusama et al.: "Serological analysis for cross-reactivity of anti human tumor associated antigen monoclonal antibody with guinea pig tumor antigen." Igaku No Ayumi.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Mikihiro Kusama et al.: "Analysis with monoclonal antibodies of the serological heterogenecity of human high molecular melanoma associated antigen." Gann.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Mikihiro Kusama et al: "Serological analysis of syngeneic antiidiotypic antibodies to murine anti alpha-fetoprotein antigen monoclonal antibodies." BIOTHERAPY.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shoji Kawabara: "Antiidiotypic monoclonal antibodies to murine anti AFP antigen monoclonal antibodies." J. of Tokyo Medical College.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Mikihiro Kusama et al.: "Antiidiotypic monoclonal antibodies to murine anti AFP monoclonal antigen-characterization and clinical application -" J. of Cancer Immunol. Immunother.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 日馬 幹弘: "抗腫瘍モノクロ-ナル抗体に対する抗イディオタイプ モノクロ-ナル抗体の作製と臨床応用" Biotherapy. 3. 329-333 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Mikihiro Kusama: "Charaterization of Syngeneic Antiidiotypic Monoclonal Antibodies to Murine AntiーHuman High Molecular Weight MelanomaーAssociated Antigen Monoclonal Antibodies" The Journal of Immunology. 143. 3844-3852 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] 日馬 幹弘: "抗ヒトAFPモノクロ-ナル抗体に対する 同系抗イディオタイプ抗体の血清学的解析" Biotherapy.

    • Related Report
      1990 Annual Research Report
  • [Publications] 川原 祥嗣: "抗ヒトAFPモノクロ-ナル抗体に対する 同系抗イディオタイプモノクロ-ナル抗体" 東京医科大学雑誌.

    • Related Report
      1990 Annual Research Report
  • [Publications] Mikihiro Kusama: "Antiidiotypic Monoclonal Antibodies to Murine Anti AFP Monoclonal antibodies" Cancer Immunol.Immunother.

    • Related Report
      1990 Annual Research Report
  • [Publications] 日馬幹弘: "抗腫瘍モノクロ-ナル抗体に対する抗イディオタイプモノクロ-ナル抗体の作製と臨床応用" Biotherapy. 3. 329-333 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Mikihiro Kusama: "Characterization of Syngeneic Antiidiotypic Monoclonal Antibodies to Murine Anti-Human High Molecular Weight Melanoma-Associated Antigen Monoclonal Antibodies" The Journal of Immunology. 143. 3844-3852 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 日馬幹弘: "モルモット腫瘍上の抗原と交叉反応を呈したヒト腫瘍関連抗原の解析" 医学のあゆみ.

    • Related Report
      1989 Annual Research Report
  • [Publications] Mikihiro Kusama: "Andlysis with Monoclonal Antibodies of the Serological Heterogeneity of Human High Molecular Weight Melanoma Associated Antigen" Gann.

    • Related Report
      1989 Annual Research Report
  • [Publications] 日馬幹弘: Biotherapy. 3. 329-333 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 日馬幹弘: 医学のあゆみ.

    • Related Report
      1988 Annual Research Report
  • [Publications] Mikihiro Kusama: Gann.

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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