MECHANISM OF ACTION OF BILE ACIDS AS PROMOTER FOR COLON CARCINOGENESIS
| Project/Area Number |
63480304
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | KOBE UNIVERSITY |
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Principal Investigator |
OHYANAGI Harumasa KOBE UNIVERSITY, ASSOCIATE PROFESSOR, 医学部, 助教授 (00030958)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAI Yoshimi KOBE UNIVERSITY, PROFESSOR, 医学部, 教授 (60093514)
SAITOH Yoichi KOBE UNIVERSITY, PROFESSOR, 医学部, 教授 (90004803)
山本 正博 神戸大学, 医学部附属病院, 講師 (40166822)
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| Project Period (FY) |
1988 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1989: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1988: ¥4,600,000 (Direct Cost: ¥4,600,000)
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| Keywords | Colon Carcinogenesis / Bile Acids / Pancreatic Acini / Small Molecular Weight G proteins / 単離膵腺房細胞 / 大腸粘膜上皮細胞 / 大腸癌 / ホスホリパーゼC |
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| Research Abstract |
The aim of this project originally was to clarify the mechanisum of promotive action of bile acids in carcinogenesis of colon. The isolated rat pancreatic acini were selected as model system for this aim because establishment of the culture system of mucous epithelial cells of rat colon was unsuccessful in final. Utilizing the pancreatic acini as the model system, it was clarified that the bile acids promote the activation of phospholipase C by the GTP-binding protein (G protein) in the intracellular signal transduction system. On the basis of these findings, we concentrated on the investigation of the interaction of bile acids with G proteins, particularly with small molecular weight G proteins, which include ras p2l. We have purified several novel small molecular weight G proteins from rat liver from which the purification of them seemed relatively easy. Among them, a G protein with a molecular weight of 24,000 was purified and its cDNA was cloned. Its monoclonal antibody is producing at present. Monoclonal antibodies of these are planned to be made. Utilizing these antibodies, the modes of existence of these small molecular G proteins and their changes under the influence of bile acids.
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Report
(3 results)
Research Products
(10 results)
