| Project/Area Number |
63480323
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Thoracic surgery
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| Research Institution | Kurume University School of Medicine |
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Principal Investigator |
OHISHI Kiroku Kurume Univ Med, Professor, 医学部, 教授 (30080766)
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| Co-Investigator(Kenkyū-buntansha) |
HISATOMI Kouichi Kurume Univ Med, Fellow, 医学部, 助手 (10183576)
ANDOU Fumihiko Kurume Univ Med, Fellow, 医学部, 助手 (80184323)
YAMANA Kazunari Kurume Univ Med, Fellow, 医学部, 助手 (60158235)
ISOMURA Tadashi Kurume Univ Med, Instructor, 医学部, 講師 (30140643)
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| Project Period (FY) |
1988 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1989: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1988: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | myocyte / culture / cardioplegic solution (CS) / albumin / in vitro / 心筋培養 / Cardioplegia(心筋保護) / Crystalloid Cardioplegic solution |
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| Research Abstract |
Myocyte culture studies were applied to improve cardioplegic solution(Cs). Rat ventricular myocardium was dissociated with 0.25% Tripsin and 0.05% Collagenase(Type I). The myocytes were cultured in 10% fetal bovine serum and Eagle's medium (Nissui) for three to four days prior to the employment for the studies.
After the incubation,the medium were exchanged to the three kinds of solutions,such as crystalloid CS(C-CS), glucose in C-CS(G-CS),and 1% bovine albumin in G-CS(GA-CS) for 20 minutes. And then the CS was exchanged to the medium again; The C-CS was most effective to arrest beating of the cells,while the favorable recovery rate of beating from the arrest was best in GA-CS group. Electron microscopic findings show the good preservation of mitochondrial morphology in GA-CS group,but not in the other two groups.
The clinical use of the GA-CS demonstrated 100% recovery of spontaneous cardiac beating after resuscitation in 8 infant patients under three months with open heart operation.
Conclusively the model of myocyte culture was useful for the improvement of CS and CS with 1% albumin was effective CS in infant for cardiac operation.
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