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CULTURE OF CEREBRAL MICROVASCULAR ENDOTHELIAL CELL AND APPLICATION FOR THE ANALYSIS OF THE CEREBRAL ISCHEMIA

Research Project

Project/Area Number 63480326
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Cerebral neurosurgery
Research InstitutionNIIGATA UNIVERSITY

Principal Investigator

TANAKA Ryuichi  NIIGATA UNIVERSITY, PROFESSOR BRAIN RESEARCH INSTITUTE, 脳研究所 (30018816)

Co-Investigator(Kenkyū-buntansha) KOIKE Tetsuo  NIIGATA UNIVERSITY, LECTURER UNIVERSITY HOSPITAL(MEDICAL), 医学部附属病院, 講師 (20158893)
Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1989: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1988: ¥4,600,000 (Direct Cost: ¥4,600,000)
KeywordsEndothelium / Culture / Microvessel / Brain / Mongolian gerbil / 脳微小血管内皮細胞 / 培養内皮細胞 / 内皮細胞障害
Research Abstract

(1) Cerebral endothelial cell damage induced by sodium arachidonate in vitro
Cytotoxic effect of sodium arachidonate on cultured microvascular endothelial cells derived from mongolian gerbil brain was measured by specific release of ^<51>Cr. Protective effect of cyclooxygenase and lipoxygenase inhibitors (indomethacin and nordihydroguaiaretic acid respectively) on cultured endothelial cells was also examined.
Sodium arachidonate injured endothelial cells at the minimal concentration of 3mug/ml, and the cytotoxicity was dependent on the concentration of sodium arachidonate and incubation time. Nordihydroguaiaretic acid inhibited the endothelial injury by sodium arachidonate. On the other hand indomethacin had no protective effect on the endothelial cells. It is posturated that the endothelial cell injury was caused by lipoxygenase product.
(2) Effect of arachidonate derivatives on cerebral endothelial permeability in vitro
Endothelial cells derived from songolian gerbil brain were cultured on collagen coated membrane filter, and the effect of arachidonate derivatives on passage of FITC-albumin through endothelial cells was examined.
Prostaglangin E_2 and F_2 alpha did not increase the permeability of endothelial cells. 15-HPETE increased the endothelial permeability slightly (p< 0.05). Leucotriene C_4 markedly increased the endothelial permeability (p<0.001). Leucotrienes may play an important role in the development of ischemic brain injury by increasing the cerebral microvascular endothelial permeability.

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] 皆河崇志: "砂ネズミ脳微小血管内皮細胞の継代培養-培養細胞の膜酵素の染色性に関する検討-" 脳卒中. 11. 89-95 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Takashi Minakawa: "Long-term Culture of Microvascular Endothelial Cells Derivedfrom Mongolian Gerbil Brain" Stroke. 20. 947-951 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 小出章: "Sodium arachidonateによる脳血管内皮細胞傷害-培養脳微小血管内皮細胞を用いた検討-" 脳と神経(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 皆河崇志: "脳微小血管内皮細胞の培養法(室田誠逸編:血管細胞の培養法とその応用)" 東京化学同人, 250 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Takashi Minakawa et al.: "Long-term culture of microvascular endothelial cells derived from mongolian gerbil brain. - Histochemical studies of cultured cells -" Japanese Journal of Stroke 11: 89-95, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Takashi Minakawa: "Long-term culture of microvascular endothelial cells derived from mongolian gerbil brain." Stroke 20: 947-951, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Takashi Minakawa et al.: "Culture of cerebral microvascular endothelial cell." "Culture of vascular cells and its application" ed. by S. Murota, pp.157-161, Tokyo Kagaku Dojin, Tokyo (1989).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Minakawa T: "Leukotriens increase cerebral microvascular endothelial Permeability in vitro" Stroke.

    • Related Report
      1989 Annual Research Report
  • [Publications] 小出章: 脳と神経.

    • Related Report
      1988 Annual Research Report
  • [Publications] Minakawa T: Stroke.

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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