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STUDY OF TRANSFORMING GROWTH FACTOR IN HUMAN OSTEOSARCOMA

Research Project

Project/Area Number 63480337
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Orthopaedic surgery
Research InstitutionTEIKYO UNIVERSITY (1989)
The University of Tokyo (1988)

Principal Investigator

HIGAKI Shozo  TEIKYO UNIVERSITY, ORTHOPEDIC SURGERY, ASSOCIATE PROFESSOR, 医学部, 助教授 (20107676)

Co-Investigator(Kenkyū-buntansha) CHO Shogen  TEIKYO UNIVERSITY, ORTHOPEDIC SURGERY, PROFESSOR, 医学部, 教授 (90082106)
飯島 卓夫  東京大学, 医学部・整外, 助手 (60176039)
小島 達自  東京大学, 医学部・整外, 助手 (30161912)
渋谷 正史  東京大学, 医科学研究所, 助教授 (10107427)
Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 1989: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1988: ¥4,100,000 (Direct Cost: ¥4,100,000)
KeywordsTRANSFORMING GROWTH FACTOR (TGF) / TGF activity / Osteosarcoma / Somatomedin / TGF-B / Prognosis / Tnausfovwy growth 6ootor / Gowatowedin / TGFーβ / Autocrie growth 6ootor / Oute3sarome / 悪性形質発現因子 / ソマトメジン
Research Abstract

The purpose of present studies were performed to clarify what kinds of transforming growth factor (TGF) concerned in human osteosarcoma, and also to clarify whether TGF assay was useful for evaluating prognosis of osteosarcoma patients.
Eighteen osteosarcoma tissues were extracted according Roberts were studied what kinds of TGFs were existing and also studied correlations between TGF activity and clinical course of these patients.
Doses of Epidermal Growth Factor (EGF) and somatomedins was assayed by RIA assay, and TGF-alpha was assayed by EGF receptor competition assay, TGF activity was assayed by soft agar colony formation activity using NRK cells with or without EGF.
RESULTS: Neither EGF nor TGF-alpha was not detected in any crude TGFs of osteosarcoma, and significant somatomedins levels were detected in every cases, and every crude TGFs were enhanced with EGF and some crude TGFs inhibited growth of porcine endothelial cells, significant doses of TGF betal should be existing in osteosarcoma.
Correlations between TGF activity and AlP-ase were not observed (r=0.44). Significant correlations were observed between TGF activity and numbers of mitotic cells (r=0.77).
TGF activity was useful to predict prognosis. Although, all cases of high TGF activity above 120 units were dead within two years, only two cases out of nine of low TGF activity below 120 units were dead within two years (p<0.01). Although all of highly enhanced cases with EGF, were dead within three years, only three cases out of nine of enhanced cases low with EGF, were dead within three years (p<0.01). These results suggested that TGF assay was useful for predicting prognosis of osteosarcoma. TGFs of osteosarcoma were mainly TGF-beta and somatomedins.

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] S.HIGAKI: "Isolation purification acid stable hepovinbinding growth bactor" Cancer Resevch(in press). (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] S.HIGAKI: "Prognsotic significance ob TGF activity -A study of 18 osteosarcoma-" Caneer Reserch(in application). (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] S.HIGAKI: "Osteoid osteoma syuthesize mainly PGE_2" Clinical Oythops related Res.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] S. HIGAKI: "ISOLATION PURIFICATION ACID STABLE HEPARINBINDING GROWTH FACTOR" CANCER RESEARCH 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] S. HIGAKI: "PROGNOSTIC SIGNIFICANCE OF TGF ACTIVITY -A STUDY OF 18 OSTEOSARCOMA-" CANCER RESEARCH 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] S. HIGAKI: "OSTEOID OSTEOMA SYNTHESIZE MAINLY PGE2" CLINICAL ORTHOPS & RELATED RESEARCH.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] S.HIGAKI: "Isolotfoie,puvification acid stoble heparin biuding THFs" Caucer Res. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] S.HIGAKI: "Progvostic sigueficduce of TGF activityーA study of 18 osteosarcowa" Caucer Res.

    • Related Report
      1989 Annual Research Report
  • [Publications] S.HIGAKI: "Osteoicl osteowa syuthesize moivly FGE_2" Clivical Orthops & releled Res.

    • Related Report
      1989 Annual Research Report
  • [Publications] 桧垣昇三: 日本整形外科学会誌. 62. S467 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] S,Higaki;etal: Caucer.

    • Related Report
      1988 Annual Research Report
  • [Publications] S,Higaki;etal: Caucer Res.

    • Related Report
      1988 Annual Research Report
  • [Publications] S,Higaki;etal: B.B.R.C.

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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