| Project/Area Number |
63480343
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Hiroshima University |
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Principal Investigator |
MIYAMOTO Yoshihiro Hiroshima Univ., School of Medicine, Associate Professor, 医学部, 助教授 (00098625)
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| Project Period (FY) |
1988 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 1990: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1989: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1988: ¥3,800,000 (Direct Cost: ¥3,800,000)
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| Keywords | Schwann cell / Sciatic nerve / Regeneration / ラミニン / 脊髄後根神経節 / Neurite Outgrouth / 線維芽細胞 / Schwann細胞 / Laminin |
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| Research Abstract |
We performed the experimental study for transplantation of cultured schwann cell. The appropriate time of transplantation is within two weeks because of the contamination by fibroblasts. We bridged 15mm gap in rat sciatic nerves by silicone tube and filled with agar, fibrin and collagen gel. No significant findings of nerve regeneration were found. Next, we added Epidermal Growth factor and Somotomedin to each materials. There were no significant findings on the regeneration of rat sciatic nerves. Then we filled with the gel containing Laminne. Four weeks later, we found soft tissues in the silicone tube and impart schwann cells under magnetic field. We added schwann cells to the gel and filled in the silicone tube. However, no significant findings were found between filling with gel only and adding schwann cells. Those reasons were suspected that the gel disappeared, schwann cell degeneerated and the gel itself inhibited for nerve regeneration.
In future, we would like to develop the technique to fill cultured schwann cells in silicone tube and to prevent cultured schwann cells from degeneration.
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