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Clinical Values of Fluorescent Techniques for Diagnoses of Perinatal Morbidities.

Research Project

Project/Area Number 63480365
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionHamamatsu University School of Medicine

Principal Investigator

TERAO Toshihiko  Hamamatsu Univ. Sch. of Med. Professor Dept. of Obst. & Gyne., 医学部 (60022852)

Co-Investigator(Kenkyū-buntansha) YAMASHITA Yutaka  Dept. of Medical Photonics, 医学部, 寄附講座教員
SUMIMOTO Kazuhiro  浜松医科大学, 医学部, 助手 (30126817)
KANAYAMA Naohiro  Assistant, 医学部, 助手 (70204550)
MAEDA Makoto  浜松医科大学, 医学部, 講師 (30135264)
NOTO Hiroshi  Lecturer, 医学部, 講師 (80115500)
林 賛育 (林 贊育)  浜松医科大学, 医学部, 助手 (40158564)
小林 隆夫  浜松医科大学, 医学部, 講師 (20107808)
早田 隆  浜松医科大学, 医学部, 講師 (60135259)
Project Period (FY) 1988 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥5,300,000 (Direct Cost: ¥5,300,000)
Fiscal Year 1990: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1989: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1988: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsMeconeum / Amniscopy / Amniotic Fluid / Meconium Aspiration Syndrome / Amniotic Fluid Embolism / Zn-Coproporpyhrin I / meconium / amnioscopy / fluorescence / amniotic fluid / meconium aspiration syndrome / 羊水 / 羊水混濁 / 羊水鏡 / 胎便 / コプロポルフィリン / 胎便吸引症候群 / 胎児仮死
Research Abstract

1. We found that meconium has characteristic fluorescence peaks at 580 nm and 630nm on excitation at 405nm. The peaks were brought about by a fluorescent substance that was later identified chemically and optically to be Zn-coproporphyrin I (ZnCP-I), excreted into meconium.
2. Based on this fluorescent characteristic, we have studied about the possibillity of in vivo detection of meconium stained amniotic fluid. This resulted in the development of a new system with an imaging fiber for clinical use to detect and determine the quantity of meconium in amniotic fluid with fiber-optic amnioscopy.
3. Using the new system, we measured the fluorescent activity of amniotic fluid in 130 pregnant women. The characteristic fluorescent peaks of meconium were recognized in 50 cases. In this group, we found high rate of PROM, IUGR, fetal distress and cord accidents.
4. Urine samples from normal neonates and neonates of Meconim Aspiration Syndrome (MAS) were collected for the measurement of UMI (Urinary Meconium Index), UFMI (Urinary Fluorescent Meconium Index defined as F_<580>-(F_<560>+F_<600>)/2 F:fluorescent intensity). False positive was observed in UMI whereas it was not found in UFMI. UFMI values were found to reflect the clinical course of neonates.
5. We measured the concentration of ZnCP-I in matermal plasma with fluorometry on HPLC. The concentration of ZnCP-I in plasma of patients with amniotic fluid embolism was significantly higher than that in normal pregnancies and immediately postpartum women. This finding shows that the detection of ZnCP-I in maternal plasma with fluorometry on HPLC is a rapid, non-invasive and sensitive method for premortal diagnosis of amniotic fluid embolism.
In summary, these fluorescent techniques of detecting ZnCP-I in amniotic fluid provide new methods for the diagnosis of meconium-related perinatal morbidities.

Report

(4 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] 住本 和博: "光工学的手法を用いた非候襲的羊水診断" 周産期学 Symposium No.6. 6. 138-144 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 住本 和博: "羊水混濁測走装置の開発" 産婦人科治療. 56. 345 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 成瀬 寛夫: "蛍光を用いた胎便吸引症候群(MAS)の特異的診断法に関する研究" 日本産科婦人科学会誌. 42. 719-726 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kanayama N.: "Determination of Zinc Copreporphyrill I in Maternal PlasntaーA new method for diagnosis of amniotic fluid embolism." Clinical Chenistry. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Horiuchi K.: "Isolation and Characterization of ZnーCoproporpltyrin I.A Magior Fluorescent Compcnent of the Porphyrin Family in meconium." Clinical Chemistry. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Terao T.: "Characteristic fluorescence spectra of amniotic fluid,fetal urine and meconium." Amrlcan Journal of Obstetrics and Gyneakgy. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sumimoto, K.: "The non-invasive diagnosis of amniotic fluid by using fluorescent technique. - The diagnosis of fetal distress related meconium stained amniotic fluid" Perinatal Medicine Tokyo. No. 6. 138 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Naruse, H.: "Study on the Specific Diagnosis of Meconium Aspiration Syndrome (MAS) with Fluorometry" Acta Obst Gynec Jpn. 42. 719 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Terao, T.: "Characteristic fluorescence spectra of amniotic fluid, fetal urine and meconium." Am. J. Obstet. Gynec.(1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Horiuchi, K.: "Isolation and Characterization of Zn-Coproporphyrin I. A Major Fluorescent Component of the Porphyrin family in meconium." Clinical Chemistry.(1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kanatama, N.: "Determination of Zinc Coproporphyrin I in Matermal Plasma - A New method for diagnosis of amniotic fluid embolism." Clinical Chemistry.(1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 成瀬 寛夫: "胎児尿由来コプロポルフィリンを指標とした羊水塞栓症の診断に関する研究" 日本産科婦人科学会雑誌. 41. S148 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 成瀬 寛夫: "蛍光測定によるMAS診断法とその指標物質に関する研究" 日本産婦人科学会関東連合地方部会報. 51. 141 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 住本 和博: "イメ-ジング機能を兼ね備えた蛍光羊水鏡の開発" 日本産婦人科学会雑誌. 43. S203 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 金山 尚裕: "Zn Coproporphyrinを利用した羊水塞栓症の新しい診断法" 日本産婦人科学会雑誌. 43. S185 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Naohiro Kanayama: "Determination of Zinc Coproporphyrin I in Maternal PlasmaーA new method for diagnosis of amniotic fluid embolism" Clinical Chemistry. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kentaro Horiuchi: "Isolation and Characterization of ZnーCoproporphyrin I.A Major Fluorescent Component of the Porphyrin Family in Meconium." Clinical Chemistry. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 寺尾俊彦: "羊水鏡" 産婦人科の実際. 38. 347 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Hiroo Naruse: "Diagnosis of Meconium Aspiration Syndrome(MAS)and Amniotic Fluid Embolism by the Fluorescent Measurement Based on the Characteristics of Porphyrins." Journal of Perinatal Medicine. 17. 104 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 成瀬寛夫: "胎便中蛍光物質を利用したMAS(Meconium Aspiration Syndrome)の診断" 日本新生児学会雑誌. 25. 146 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 成瀬寛夫: "胎便中蛍光物質の同定・精製および臨床応用に関する研究" 日本新生児学会雑誌. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 成瀬寛夫: "蛍光を用いた胎便吸引症候群(MAS)の特異的診断法に関する研究" 日本産科婦人科学会雑誌. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 寺尾俊彦: 産婦人科の実際. 38. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 住本和博: 産婦人科治療. 56. 345 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 住本和博: 周産期Symposium. 6. 138-144 (1988)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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