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The Fundamental and Clinical Study of the Facial Paralysis Caused by Herpes Virus

Research Project

Project/Area Number 63480381
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Otorhinolaryngology
Research InstitutionEhime University

Principal Investigator

YANAGIHARA Naoki  Ehime University, School of Medicine, Otolaryngology, Professor, 医学部, 教授 (40025581)

Co-Investigator(Kenkyū-buntansha) OZAWA Tetsuo  Ehime University, School of Medicine, Otolaryngology, Assistant Instructor, 医学部, 助手 (70153525)
NAKAMURA Koshiro  Ehime University, School of Medicine, Otolaryngology, Assistant Instructor, 医学部, 助手 (10164295)
YUMOTO Eiji  Ehime University, School of Medicine, Otolaryngology, Assistant Professor, 医学部・附属病院, 講師 (40116992)
GYO Kiyohumi  Ehime University, School of Medicine, Otolaryngology, Assistant Professor, 医学部・附属病院, 講師 (00108383)
佐伯 忠彦  愛媛大学, 医学部附属病院, 助手 (60186913)
木谷 伸治  愛媛大学, 医学部附属病院, 助手 (30169867)
佐藤 英光  愛媛大学, 医学部, 助手 (30187223)
Project Period (FY) 1988 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1990: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1989: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1988: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsFacial Paralysis / Bell's Palsy / Ramsay Hunt's Syndrome / Herpes Simplex virus / Varicella Zoster Virus / ペル麻痺 / ヘルペスウイルス / へルペスウイルス
Research Abstract

1) Biopsy specimens of the middli ear mucosa were taken during decompression operations in the 11 patients with Ramsay Hunt's syndrome and the 13 patients with Bell's palsy. The mucosas were investigated by the indirect immunofluorescent technique for detection of the HSV antigens and Hematoxyline Eosin (HE) staining. Specific VZV antigens were demonstrated in the cytoplasm and nucleus of elliptically shaped cells and round cells in the two patients with Ramsay Hunt's syndrome. But no virus antigens and no inflammatory changes were observed in Bell's palsy.
2) Lymphocytes were separated in 26 patients with Bell's palsy. The KOS strain of HSV type 1 was inactivated and added as HSV antigen. ^3H-tymidine was added to each lymphocyte and radioactivity was measured. Median cpm's of triplicate cultures were used to calculate the stimulation index (SI). The mean SI values began to increase between 1 and 7 days and became highest between 8 and 14 days after the onset. This result is similar to the report in patients with recurrent herpes labialis.
3) The KOS strain of HSV type 1 was inoculated to the tongue or the auricles of the balb/c mouse previously treated by the intraperitoneal injection of cyclophosphamide. The facial nerves were investigated by the indirect immunofluorescent technique for detection of the HSV antigens and HE staining. Inflammatory changes were observed in the site of the inoculation, which includes round cell infiltration and intranuclear inclusion bodies in the geniculate ganglion and vacuolar degeneration of nerve fiber. The viral geniculate ganglionitis was induced in 21.4%, 30,0%, 37.5% of the animals inoculated to the tongue, unilateral or bilateral auricles, respectively.

Report

(4 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] 松本 康、柳原 尚明 他: "Bell麻痺のMR像" 耳鼻臨床. 83. 1361-1366 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 中村 光士郎、柳原 尚明: "顔面神経麻痺の病因と麻痺程度の評価法" 耳鼻咽喉科・顔頚部外科. 62. 539-543 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yasuo Fujiwara Naoaki Yanagihara Takesi Kurata: "Hiddle car mucosa in Ramsay Hunt syndrome" Annals Otol Rhinol Laryngol. 99. 359-362 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 杉田 俊明、柳原 尚明、藤原 康雄: "顔面神経における単純ヘルペスウイルス感染の実験的研究" Ear Res Jpn. 21. 155-156 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 杉田 俊明、藤原 康雄、柳原 尚明: "実験的HSV感染による顔面神経麻痺に関する研究" Facial Nerve Res Jpn. 10. 33-36 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Fujiwara, Yasuo, Yanagihara, Naoaki, Kurata, Takesi: "Pathological findings of middle ear mucosa in Bell's palsy" Facial Nerve Res Jpn. 8. 37-40 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yanagihara, Naoaki: "Incidence of Bell's palsy" Ann Otol Rhinol Laryngol 97, suppl. 137. 3-4 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Nakamura, Kshiro, Yanagihara, Naoaki: "Neutralization antibody to herpes simplex virus type 1 in Bell's palsy" Ann Otol Rhinol Laryngol 97, suppl. 137. 18-21 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Matsumoto, Yasusi, Pulec, Jack, Patterson, Michael, Yanagihara, Naoaki: "Facial nerve biopsy for etiologic clarification of Bell's palsy" Ann Otol Rhinol Laryngol 97, suppl. 137. 22-27 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Fujiwara, Yasuo: "An experimental study of herpes simplex virus infection in the facial nerve" Jounal of Otolaryngol of Jpn,. 92. 1026-1035 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Fujiwara, Yasuo, Yanagihara, Noaoaki, Kurata, Takesi: "The experimental study of herpes simplex virus infection in the facial nerve" Facial Nerve Res Jpn. 9. 51-54 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Fujiwara, Yasuo, Yanagihara, Naoaki, Kurata, Takesi: "Middle ear mucosa in Ramsay Hunt syndrome" Annals Otol Rhinol Laryngol. 99(5). 359-362 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sugita, Tosiaki, Yanagihara, Naoaki, Fujiwara, Yasuo: "The experimental study of herpes simplex virus infection in the facil nerve" Ear Res Jpn. 21. 155-156 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sugita, Tosiaki, Fujiwara, Yasuo, Yanagihara, Naoaki: "The experimental study of the facial nerve paralysis by herpes simplex virus infection" Facial Nerve Res Jpn. 10. 33-36 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 松本 康,柳原 尚明ほか: "Bell麻痺のMR像" 耳鼻臨床. 83. 1361-1366 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 中村 光士郎,柳原 尚明ほか: "顔面神経麻痺の病因と麻痺程度の評価法" 耳鼻咽喉科・頭頚部外科. 62. 539-543 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yasuo Fujiwara;Naoaki Yanagigara;Takesi Kurata: "Middle ear mucosa in Ramsag Hunt syndrome" Annals Otol Rhinol Laryngol. 9ー9. 359-362 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 杉田 俊明,柳原 尚明,藤原 康雄: "顔面神経における単純ヘルペスウイルス感染の実験的研究" Ear Res Jpn. 21. 155-156 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 杉田 俊明,藤原 康雄,柳原 尚明: "実験的HSV感染による顔面神経麻痺に関する研究" Facial Nerve Res Jpn. 10. 33-36 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 藤原康雄: "顔面神経における単純ヘルペスウイルス感染の実験的研究" 日本耳鼻咽喉科学会会報. 92. 1026-1035 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yasuo Fjiwara他: "Middle Ear Mucosu in Ramsay Hunt's Syndrome" Annals of Otology,Rhinology & Laryngology.

    • Related Report
      1989 Annual Research Report
  • [Publications] 藤原康雄 他: "顔面神経における単純ヘルペウイルス感染の実験的研究" Facial nerve research. 9. 51-54 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 杉田俊明 他: "顔面神経における単純ヘルペスウイルス感染の実験的研究" Ear Research.

    • Related Report
      1989 Annual Research Report
  • [Publications] Naoaki Yanagihara 他: Annals of Otology,Rinology & Laryngology. 97. 3-7 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Naoaki Yanagihara 他: Annals of Otology,Rinology & Laryngology. 97. 14-17 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Koshiro Nakamura 他: Annals of Otology,Rinology & Laryngology. 97. 18-21 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yasushi Matsumoto 他: Annals of Otology,Rinology & Laryngology. 97. 22-27 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yasuo Fujiwara 他: Annals of Otology,Rinology & Laryngology.

    • Related Report
      1988 Annual Research Report
  • [Publications] 藤原康雄 他: Facial N Res.Jpn.8. 37-40 (1988)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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