Molecular Biological Research of Retinoblastoma Cell Fusion between Retinoblastoma and Non-Malignant Cell
| Project/Area Number |
63480393
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Ophthalmology
|
|---|
| Research Institution | Shinshu University |
|---|
Principal Investigator |
TOTSUKA Seiichi Shinshu Univ. Hosp. Lecturer, 医学部・附属病院, 講師 (50107629)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ISHIHARA Atsushi Shinshu Univ. Sch. of Med., 医学部, 助手 (00168246)
|
|---|
| Project Period (FY) |
1988 – 1990
|
| Project Status |
Completed (Fiscal Year 1990)
|
| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥5,200,000)
Fiscal Year 1990: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1989: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1988: ¥4,000,000 (Direct Cost: ¥4,000,000)
|
|---|
| Keywords | Retinoblastoma / Cell Fusion / Cell Culture / Molecular Biology |
|---|
| Research Abstract |
Retinoblastoma (RB) is one of the most common intraocular malignant tumor. Recently with the analysis of the pedigree, chromosomes, and DNA, it is thought that the lack of a depressor gene, RB gene, produce RB tumor.
With cell fusion technique, we confirmed that RB is induced by the lack of gene(s). RB Y79 cell line was fused with normal human fibroblasts, and the hybrids between them were examined microscopically. Those were all fibroblastic in appearance and grew well on the culture dishes and not well in suspension. So it was confirmed that RB is induced by the lack of gene(s).
We also tried to do cell culture of RB from the three clinical cases, and in one case, we succeeded in culture. Two types of the cells were seen, the one was fibroblastic and showed two layer growth partly. The other was with many processes, and similar to a nerve cell. The latter had a tendency to stop growing and decreased in number.
|
Report
(4 results)
Research Products
(9 results)
