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Study for speciality of mechanism of biological response in developing retina and vitreous

Research Project

Project/Area Number 63480397
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Ophthalmology
Research InstitutionSchool of Medicine, Keio University

Principal Investigator

UEMURA Yasuo  School of Medicine, Keio University, Professor, 医学部, 教授 (30051006)

Co-Investigator(Kenkyū-buntansha) AZUMA Noriyuki  School of Medicine, Keio University,, 医学部, 助手 (10159395)
TANAKA Yasuhiko  School of Medicine, Keio University, Associate Professor, 医学部, 講師 (30051551)
気賀沢 一輝  慶應義塾大学, 医学部, 助手 (20129406)
桂 弘  慶應義塾大学, 医学部, 助手 (40129391)
Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1989: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1988: ¥5,200,000 (Direct Cost: ¥5,200,000)
KeywordsBeagle puppy / Oxygen induced retinopathy / Macrophages / Collagen / Hyaloid vessels / Myofibroblasts / Retinal folds / ビーグル犬.未熟児網膜症 / 新生血管 / 無血管帯 / 網膜血管内皮細胞 / I型コラーゲン
Research Abstract

We developed oxygen induced retinopathy in beagle puppy with 95%-100% oxygen exposure for 4 days after birth. About 50% puppies showed vitreoretinal disorders. Electropathohistologically, cellular amoeboid process, rich glycogen granules, and poorly differentiated basement membrane, were observed, which were common findings of noevascularization of human retina. However, such cellular budding phenomenon was observed not only in peripheral but central retina. And no significant morphological deference was recognized between peripheral budding and central one.
Around neovascularization, many macrophages and collagen fibrils were detected. Rich lysosome granules and deviation of nucleus were observed in macrophages. Collagen fibers, which was quite different from normal vitreous collagen were very bold and showed 65nm period's band which thought to be the type I collagen. Therefore these macrophages seemed to not conduct the neovascularization but to phagocytize foreign bodies.
Some species showed not only retinal vasculature but hyaloid vasculature had been proliferated. Four of them formed retinal folds along hyaloid system.
Electron-microscopically, retinal folds adhered tightly with proliferated hyaloid vessels and collagen fibers. Some cells, surrounded neovascularization, showed positive in actin immunohistochemically which suggested to exist myofibroblasts. Therefore retinal fold and/or dragged retina may be formed by attachment of fibroblasts and active shrinkage of myofibroblasts.
This finding was very interesting and very important to investigate not only ROP but congenital retinal folds with proliferative vitreoretinal changes of developing stages.

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Noriyuki Azuma: "Histochemical and Electron Microscopic Studies on the Hyalocytes in the Developing Human Vitreous." Invest.Ophthalmol.Vis.Sci.(suppl). 351. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Noriyuki Azuma: "Histochemical Studies on Hyaluronic Acid in the Developing Human Retina." Graefe's Archive Clin.Exp.Ophthalmol.228. 158-160 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 田中靖彦: "網膜色素上皮の働き" 眼科. 31. 1233-1238 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 東範行: "朝顔症候群と類縁疾患" 眼科. 31. 235-246 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 東範行: "乳頭領域の陥凹を伴わない朝顔症候群類似の乳頭部先天異常" 臨床眼科. 43. 1377-1384 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 東範行: "硝子体疾患-第一次硝子体過形成遺残と家族性滲出性硝子体網膜症-" あたらしい眼科. 6. 1803-1812 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 東範行: "目でみる小児科診療,第一次硝子体過形成遺残" 中外医学. 43. 26-29 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 東範行: "Monosodium-L-glutamateによる幼若ラット網膜・視神経の変性に関する形態学的および免疫組織化学的研究" 日本眼科学会雑誌. 93. 72-79 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Noriyuki, Azuma: "Histochemical and Electron Microscopic Studies on the Hyalocytes in the Developing Human Vitreous." Invest. Ophthalmol. Vis. Sci. (suppl) 351, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Noriyuki, Azuma: "Histochemical Studies on Hyaluronic Acid in the Developing Human Retina." Graefe's Archive Clin. Exp. Ophthalmol., 228:158-160, 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Noriyuki, Azuma: "Morning glory Syndrome" Ganka, 31:235-246, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Noriyuki, Azuma: "Morphological and Immunohistochemical Study of Degeneration with Monosodium-L-glutamate of Immature Rat's Retina and Optic Nerve" Acta Jap. Ophthalmol., 93:72-79, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 東範行、植村恭夫: "乳頭領域の陥凹を伴わない朝顔症候群類似の乳頭部先天異常" 臨床眼科. 43. 1377-1384 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 田中靖彦: "眼科の基礎ー卒後研修医のためにVI.視覚・網膜機能の基礎4.網膜色素上皮の働き" 眼科. 31. 1233-1238 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 東範行: "硝子体疾患ー第一次硝子体過形成遺残と家族性滲出性硝子体網膜症ー" あたらしい眼科. 6. 1803-1812 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 東範行: 日本眼科学会雑誌. 93. 72-79 (1989)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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