Studies on mechanisms of proliferation, differentiation and calcification of chondrocytes using established clonal cell lines.
| Project/Area Number |
63480411
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional basic dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
TAKIGAWA Masaharu Fac. of Dentistry, Dept. of Biochemistry, Assistant Prof., 歯学部, 講師 (20112063)
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| Co-Investigator(Kenkyū-buntansha) |
ASADA Akira Fac. of Dentistry, Research Resources Center, Assistant Prof., 歯学部, 講師 (50028734)
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| Project Period (FY) |
1988 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1989: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1988: ¥4,700,000 (Direct Cost: ¥4,700,000)
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| Keywords | Established cell line / Chondrocytes / Osteoblasts / Proteoglycan / Collagen / Chondrosarcoma / Calcification / Alkaline phosphatase / コラーゲン / アルカリホスファターゼ |
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| Research Abstract |
1) Three clonal cell lines with differences in responsiveness to parathyroid hormone (PTH), alkaline phosphatase activity and ability to produce an endothelial cell growth inhibitor(s) during more than 3 years in culture were established from growth cartilage (GC) of mouse ribs. MGC/Tl.17 cells had high activity of alkaline phosphatase, an ability to calcify and epidermal growth factor (EGF) receptors and responded well to epidermal growth factor, transforming growth factor beta. MGC/Tl.18 cells responded well to parathyroid hormone and produced sulfation factor. MGC/Tl.4 cells produced endothelial cell growth factor and sulfation factor. Glycosaminoglycan (GAG) syntheses in the three clonal lines were much lower than that of primary cultures of GC cells. The three clonal lines mainly synthesized type I collagen. Because of their different properties, these cell lines should be useful for studies on endochondral ossification, the actions of PTH on skeletal cells and anti-angiogenesis factors.
2) A clonal cell line with cartilage phenotypes during more than 3 years in culture was established from a human chondrosarcoma. The clonal line, named HCS-2/8, formed synthesized cartilage-type proteoglycans and collagen types II and XI which are typical cartilage phenotypes. Like rabbit costal chondrocytes in primary culture, they also responded well to insulin-like growth factors I and II, transforming growth factor beta and retinoic acid. HCS-2/8 cells had low alkaline phosphatase activity and did not calcify in the absence of ascorbic acid but the enzyme activity increased markedly in the presence of the vitamin. The cells produced inhibitors of alkaline phosphatase and calcification. Because of these properties, the cell line should be useful for studies on endochondral ossification.
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Report
(3 results)
Research Products
(21 results)
