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The immunological investigations concerning the pathological mechanisms of periodontal diseases

Research Project

Project/Area Number 63480420
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Conservative dentistry
Research InstitutionTokyo Medical and Dental University

Principal Investigator

ISHIKAWA Isao  Tokyo Medical and Dental University Dental School Professor, 歯学部, 教授 (10014151)

Co-Investigator(Kenkyū-buntansha) TAKEFUTA Wataru  Tokyo Medical and Dental University Dental School Assistant, 歯学部, 助手 (00179665)
WATANABE Hisashi  Tokyo Medical and Dental University Dental School Lecturer, 歯学部, 講師 (40143606)
HAGIWARA Satsuki  Tokyo Medical and Dental University Dental School Lecturer, 歯学部, 講師 (70134715)
URAGUCHI Ryoji  Tokyo Medical and Dental University Dental School Lecturer, 歯学部, 講師 (90111608)
Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 1989: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1988: ¥5,200,000 (Direct Cost: ¥5,200,000)
KeywordsELISA method / Serum IgG antibody / Leukocytes function / Platelet-activating-factor (PAF) / 15-HETE / Immunoblotting / Gene cloning / DNA Probe / 重度歯周炎 / 歯周病原性菌 / 生体応答 / 抗体価 / 走化能 / 殺菌能
Research Abstract

Our analysis concerning the microbiological and immunological mechanisms of periodontal diseases yielded many fruitful results. These results are as follows.
1) Serum IgG antibodies of severe periodontitis patients were measured to seven periodontopathic bacteria with enzyme-linked immunosorbent assay(ELISA).
The findings demonstrated the pathological role of B. gingivalis and A. actino mycetemcomitans played in periodontal disease.
2) The degree of definitive neutrophil chemotaxis in Down' s syndrome(DS) and its relationship to periodontal disease progression was confirmed by our studies.
3) Platelet-activating factor(PAF), a biological active lipid was detected in inflamed human gingival tissue directly associated with the occurrence and persistence of periodontal inflammation.
4) Synthesis competence of 15-hydroxy-eicosatetraenoic acid(15-HETE) by polymorphonuclear leukocytes(PMLs) of severe periodontitis patients was measured to observe 15-lipoxygenase activity. Juvenile and rapidly pro … More gressive periodontitis groups reported lower 15-HETE synthesis than the age-matched controls. The results are relevant to those communications concerning juvenile and rapidly progressive periodontitis with abnormal PMNL function.
5) Sonicated whole cell extracts and outer membrane proteins from B.gingivalis W50, Veionella purvula ATCC 17745 and A. actinomycetemcomitans ATCC 9710, and sonicated whole cell extract from B. gingivalis 381 were analyzed by the immunoblot technique using sera of patients with diverse periodontal diseases and control subjects.
Several major bands to which the specific antigens reacted more frequently with sera from periodontitis patients were recorded. Monitoring of antibody response to specific antigens may be significant to assess human periodontal disease progress.
6) Clones of genes with antigen reactive from sera of advanced destructive periodontitis(ADP) patients to construct a phage library of A.actinomycetemcomitans Y4 DNA were screened by the immunochemical analysis. This investigation indicated a distinctive DNA sequence encoding an A.a. Y4 antigen strongly reactive to the sera of the ADP patients. Hence, serve as a DNA probe in diagnosis of periodontitis. Less

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (45 results)

All Other

All Publications (45 results)

  • [Publications] I.Ishikawa: "Diversity of IgG antibody responses in the patients with various types of periodontitis" Advances in Dental Research. 2. 334-338 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 堀部元雄: "ELESA法の歯周疾患診断への応用" 歯科ジャ-ナル. 28. 543-549 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kazuyuki Noguchi: "Imparied polymorphonuclear leukocyte 15-lipoxygenase activity in juvenili and rapidly progressive periodontitis" Prostaglandins Leukotrienes and Essential Fatty Acids. 33. (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] T.Okano: "Quantitative evaluation of proximal bone lesions using digital subtraction radiography" Dentomaxillofacial Radiology. 17. 99-103 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Y.Izumi: "Defective neutrophil chemotaxis in Down's syndrome patients and its relationship to periodontal destruction" Journal of Periodontology. 60. 238-242 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] K.Noguchi: "The detection of platelet-activating factor in inflamed human gingival tissue" Archives of Oral Biology. 34. 37-41 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] S.Natasasmita: "Serum IgG level against streptococcus mutans in subjects with caries experience" The Bulletin of Tokyo Medical and Dental University. 36. 27-33 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] H.Watanabe: "Detection of emmunodominat antigens of periodontopathic bacteria in human periodontal disease" Oral Microbiology and Immunology. 4. 159-164 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] H.Watanabe: "Antigens of Actinobicilus actinomycetemcomitans identified by immunoblotting with sera from patients with localized human juvenile periodontitis and generalized severe periodontitis" Archives of Oral Biology. 34. 649-656 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 石川烈: "歯周病の診断と治療" 歯界展望. 74. 1163-1172 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 荒川真一: "重度進行性歯周炎患者末梢血血清と反応するActinobacillus actinomycetemcomitans Y4株抗原遺伝子のクロ-ニング" 口腔病学会雑誌. 56. 494-512 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] M.Umeda: "Improved detection of oral spirochetes with an anaerobic culture method" Oral Microbiology and Immunology(in press). 5. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kazuyuki Noguchi: "The presence of platelet-activating factor(PAF)in inflamed human gingival tissue(Recent advances in clinical periodantalogy)" Elsevier Science Publishers B.V.(J.Ishikawa et al.editors), 379-382 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] I.Ishikawa, H.Watanabe, M.Horibe and Y.Izumi.: "Diversity of IgG antibody responses in the patients with various types of periodontitis." Advance in Dental Research. 2:334-338, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] M.Horibe and I.Ishikawa.: "Application of ELISA method to the diagnosis of periodontitis diseases." The Journal of Dental Medicine (Japan) 28:543-549, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] T.Okano, M.Ohki, T.Mera, H.Soejima, I.Ishikawa and N.Yamada.: "Quantitative evaluation of proximal bone lesions using digital subtraction radiography." Dentomaxillofacial Radiology, 17:99-103, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] K.Noguchi, I.Morita, I.Ishikawa and S.Murota.: "Impaired polymorphonuclear leukocyte 15-lipoxygenase activity in juvenile and rapidly progressive periodontitis." Prostagrandins Leukotrienes and Fatty Acids. 33:137-141, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] K.Noguchi, I.Morita, I.Ishikawa and S.Murota.: "The presence of platelet-activationg factor (PAF) in inflamed human gingival tissue." Recent advances in clinical periodontology. Elsevier Science Publishers B.V.(Biomediccal Division), J.Ishikawa et al.editors, 379-382, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] K.Noguchi, I.Morita and S.Murota.: "The detection of platelet-activating factor in inflamed human gingival tissue." Archives of Oral Biology. 34:37-41, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Y.Izumi, S.Sugiyama, O.Shinozuka, Y.Yamazaki, T.Ohyama and I.Ishikawa.: "Defective neutrophil chemotaxis in Down's syndrome patients and its relationship to periodontal destruction." Journal of Periodontology, 60:238-242, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] S.Natasasmita, S.Soemohatmato and H.Watanabe.: "Serum IgG level against Streptcoccus mutans in subjects with caries experience." The Bulletin of Tokyo Medical and dental University. 36:27-33, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] H.Watanabe, P.D.Marsh and L.Ivanyi.: "Antigens of Actinobacillus actinomycetemcomitans identified by immunoblotting with sera from patients with localized human juvenile periodontitis and generalized severe periodontitis." Archives of Oral Biology, 34:649-656, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] H.Watanabe, P.D.Marsh and L.Ivanyi.: "Detection of Immunodominant antigens of periodontopathic bacteria in human periodontal disease." Oral Microbiological Immunology. 4:159-164, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] I.Ishikawa.: "Recent advances of periodontal diagnosis and treatment." Dental Outlook. 74:1163-1172, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] S.Arakawa.: "Gene Cloning of an actinobacillus actinomycetemcomitans Y4 antigen that reacts to peripheral blood sera in advanced destructive periodontitis patients." The Journal of the Stomatological Society, Japan. 56.494-512, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] M.Umeda, I.Ishikawa, Y.Benno, T.Mitsuoka.: "Improved detection of oral spirochetes with an anaerobic culture method." Oral Microbiological Immunology. 5:1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] N.Laosrisin, K.Nakashima and I.Ishikawa.: "Detection of bacteroides gingivalis antigenic proteins by immunoblotting analysis." Journal of Periodontology. 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] I.Ishikawa: "Diversity of IgG antibody responses in the patients with various types of periodontitis" Advances in Dental Research. 2. 334-338 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] 堀部元雄: "ELISA法の歯周疾患診断への応用" 歯科ジャ-ナル. 28. 543-549 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kazuyuki Noguchi: "Impaired polymorphnuclear leukocyte 15-lipoxygenase activity in juvenile and rapidly progressive periodoutitis" Prostaglandins Leukotrienes and Essential Fatty Acids. 33. (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Okano: "Quantitative evaluation of proximal bone lesions using digital subtraction radiography" Dentomaxillofacial Radiology. 17. 99-103 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Y.Izumi: "Defective neutrophil chemotaxis in Doron's syndrome patients and its relationship to periodontal destruction" Journal of Periodontology. 60. 238-242 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] K.Noguchi: "The detection of platelet-activating factor in inflamed human gingival tissue" Archives of Oral Biology. 34. 37-41 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] S.Natasasmita: "Serum IgG level against streptococcus mutans in subjects with caries experience" The Bulletin of Tokyo Medical and Dental University. 36. 27-33 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] H.Watanabe: "Detection of immunodominant antigens of periodontopathic bacteria in human periodontal disease" Oral Microbiology and Immunology. 4. 159-164 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] H.Watanabe: "Antigens of Actinobicilus actinomycetemcomitans identified by immunoblotting with sera from patients with localized human juvenile periodontitis and generalized severe periodontitis" Archives of Oral Biology. 34. 649-656 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 石川烈: "歯周病の診断と治療" 歯界展望. 74. 1163-1172 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 荒川真一: "重度進行性歯周炎患者末梢血血清と反応するActinobacillus actinamycetemcomitansY4株抗原遺伝子のクロ-ニング" 口腔病学会雑誌. 56. 494-512 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] M.Umeda: "Improved detection of oral spirochetes with an anaerobic culture method" Oral Microbiology and Immunology. 5. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Narongsak Laosrisin: "Detection of Bacteroides gingivalis antigenic proteins by immunoblotting analysis" Journal of Periodontology. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kazuyuki Noguchi: "The Presence of platelet-activating factor(PAF)in inflamed human gingival tissue(Recent advances in clinical periodon,tology)" Elsevier Science Publishers B.V.(J.Ishikawa et al editors), 379-382 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] 石川烈: 日本歯科医師会雑誌. 40. 785-798 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] 堀部元雄;石川烈: 歯科ジャーナル. 28. 543-549 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] I.ISHIKAWA;H.WATANABE;M.HORIBE;Y.IZUMI: Advances in Dental Research. 2. 334-338 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] H.WATANABE;P.D.MARSH;L.IVAN.TI: Archives of Qral Biology. (1989)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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