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Molecular Pathology of Periodontal Diseases

Research Project

Project/Area Number 63480421
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Conservative dentistry
Research InstitutionOkayama University

Principal Investigator

MURAYAMA Yoji  Okayama Univ. Dental School, Professor, 歯学部 (50029972)

Co-Investigator(Kenkyū-buntansha) GOTO Hiroyuki  Okayama Univ. Dental School, Instructor, 歯学部, 助手 (90205609)
KURIMOTO Keiji  Okayama Univ. Dental School, Instructor, 歯学部, 助手 (80161769)
NAGAI Atsushi  Okayama Univ. Dental School, Instructor, 歯学部, 助手 (70172484)
KURIHARA Hidemi  Okayama Univ. Dental School, Lecturer, 歯学部附属病院, 講師 (40161765)
NOMURA Yoshio  Okayama Univ. Dental School, Assistant Professor, 歯学部, 助教授 (50107075)
磯島 修  岡山大学, 歯学部, 助手 (90176256)
Project Period (FY) 1988 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 1990: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1989: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1988: ¥3,700,000 (Direct Cost: ¥3,700,000)
Keywordsperiodontal disease / LFA-l family / neutrophilis function / HLA-class II antigen / host defensive function / gene diagnosis / HLAクラスII抗原 / LAD(白血球接着不全症) / LFA-1ファミリ-分子 / RFLP(DNA制限フラグメント多型分析) / LFA-1 / Mac-1 / p150 / 95 / 若年性歯周炎
Research Abstract

Study 1. Neutrophil cell surface glycoprotein (LF-1 family) anomaly
1) The quantity of LFA-1 family beta-subunit of the neutrophil cell surface glycoprotein expressed on the cell membrane was studied in 28 Patients with various types of periodontal disease by Western blotting and Flow cytometry using the monoclonal antibody against the beta-subunit.
2) In patients with early-onset periodontitis, the LFA-1 family beta-subunit was not fully expressed on the cell surface even after the stimulation with fMLP.
3) The LFA-1 family beta-subunit is proposed as a possible marker in a novel typing for a certain periodontal disease.
4) The restriction fragment length polymorphism (RFLP) analysis of LFA-1 family beta gene may make a gene diagnosis of such a periodontal disease subgroup possible.
Study 2. RFLP analysis of HLA genes
1) The possibility of gene diagnosis for a specific periodontal disease subgroup was explored by the detection of variations in the HLA class II beta-chain genes.
2) The RFLP analysis of HLA-DQbeta gene digested with Bam H1 revealed that genetic variation existed in 4 patients with early-onset periodontitis out of 96 subjects.
3) Gene variations may be useful as markers for novel gene diagnosis for a certain subgroup of periodontal disease.
Study 3. Case reports of families with early-onset periodontitis
1) Out of 3 daughters with juvenile periodontitis and their parents with adult periodontitis, 4 persons except father did not raise the level of serum IgG against periodontopathic bacteria.
2) A mother and elder daughter with early-onset periodontitis and periodontally healthy younger daughter revealed host defensive function of T cell activation.
3) The neutrophils from a mother and elder daughter with early-onset periodontitis and a periodontally healthy younger daughter showed characteristically reduced chemotactic activity against fMLP.

Report

(4 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • Research Products

    (55 results)

All Other

All Publications (55 results)

  • [Publications] Y.Murayama,et al: "Serum immunoglobulin G antibody to periodontal bacteria." Adv.Dent.Res.2. 339-345 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Y.Katsuragi,et al.: "Neutrophil functions in patients with severe periodontal disease." Adv.Dent.Res.2. 359-363 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] S.Takashiba,et al.: "Polymorphism of compromised hosts in periodontitis patients" Recent Advances in Clinical Periodontology. 383-386 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Y.Mizushima,et al.: "Serum immunoglobulin G antibody in response to change of periodontal microflora." Recent Advances in Clinical periodontology. 673-676 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] S.Kokeguchi,et al.: "Cell surface protein antigen from <Wolinella recta>___ー ATCC 33238." J.Clin.Microbiol.27. 1210-1217 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 小林 充治: "好中球細胞膜糖蛋白(LFAー1ファミリ-)が関与する歯周病の病態研究" 日歯周誌. 32. 370-385 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 高柴 正悟: "ヒト白血球抗原(HLA)遺伝子の製限断片長多型(RFLP)解析による歯周病病態の分子生物学的研究" 日歯周誌. 32. 386-401 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 村山 洋二: "歯周病の診断と治療のための宿主防御細胞機能" 日本歯科評論. 568. 77-85 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 村山 洋二: "歯周疾患と生体防御" 歯科ジャ-ナル. 31. 793-805 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 小林 充治,村山 洋二: "好中球膜糖蛋白を“リスク因子"とする歯周病症例" 歯科ジャ-ナル. 31. 843-850 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] F.Nishimura.et al.: "A family study of a mother and daughter with increased susceptibility to earlyーonset periodontitis:microーbiological,host defensive,and genetic analyses." J.Periodontol.61. 755-765 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] S.Kokeguchi,et al.: "Isolation and partial characterization of a 39KDa major outer membrance protein of <Actinobacillus actinomycetemcomitans>___ー Y4." FEMS Microbiology Letters.77. 85-90 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] S.Kokeguchi,et al.: "Puritication and characterization of two major outer membrance proteins from <Bacteroides gingivalis>___ー." Dentistry in Japan. 27. 1-6 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M.Miyamoto,et al.: "Molecular cloning and sequence analysis of the 53ーkilodalton antigen of <Treponema denticola>___ー." Infect.Immun.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] H.kurihara,et al: "Humoral immune response to an antigen from <Porphyromonas gingivalis>___ー 381 in periodontal disease." Infect.Immun.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 阿久津 功: "歯周病の発症と進行におけるT細胞の関わりについての研究" 日歯周誌. 33. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Y. Murayama et al.: "Serum immunoglobulin G antibody to periodontal bacteria" Adv. Dent. Res.2. 339-345 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Y. Katsuragi et al.: "Neutrophil functions in patients with severe periodontal disease" Adv. Dent. Res.2. 359-363 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] S. Takashiba et al.: "Polymorphism of compromised hosts in periodontitis patients" Elsevier Science Publishers. B. V.,. 383-386 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Y. Mizushima et al.: "Serum immunoglobulin G antibody in response to change of periodontal microflora" Elsevier Science Publishers. B. V.,. 673-676 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] S. Kokeguchi et al.: "Cell surface protein antigen from Wolinella recta ATCC 33238" J. Clin. Microbiol.27. 1210-1217 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M. Kobayashi: "Study of a periodontal disease related to neutrophil cell surface glycoprotein (LFA-l family) anomaly" J. Japan. Ass. Periodont.32. 370-385 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] S. Takashiba: "Molecular biological study of periodontal diseases by restriction fragment length polymorphism (RFLP) analysis of human leukocyte antigen (HLA) genes" J. Japan. Ass. Periodont.32. 386-401 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Y. Murauama: "Considerations of host^'s defensive cell functions for diagnosis and treatment of periodontal disease" The Nippon Dental Review. 568. 793-805 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Y. Murayama: "Periodontal disease and host^'s defensive functions" J. Dental Medicine (Japan). 31. 793-805 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M. Kobayashi and Y. Murayama: "A case of periodontitis with a "risk factor" in neutrophil membrane glycoproteins" J. Dental Medicine. 31. 843-850 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] F. Nishimura et al.: "A family study of a mother and daughter with increased susceptibility to early-onset periodontitis : microbiological, immunological, host defensive, and genetic analyses" J. Periodontol.61. 755-765 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] S. Kokeguchi et al.: "Isolation of a 39kDa major outer membrane protein of Actinobacillus actinomycetemcomitans Y4" FEMS Microbiology Letters. 77. 85-90 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] S. Kokeguchi et al.: "Purification and characterization of two major outer membrane proteins from Bacteroides Bgingivalis" Dentistry in Japan. 27. 1-6 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M. Miyamoto: "Molecular cloning and sequence analysis of the 53-kilodalton antigen of Treponema denticola" Infect. Immun.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] H. Kurihara et al.: "Humoral immune response to an antigen from Porphyromomas Bgingivalis 381 in periodontal disease" Infect. Immun.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] I. Akutsu: "Studies on the role of T cells in the onset and progression of periodontal disease" J. Japan. Ass. Periont.33. (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Y.Murayama,et al.: "Serum immunoglobulin G antibody to periodontal bacteria." Adv.Dent.Res.2. 339-345 (1988)

    • Related Report
      1990 Annual Research Report
  • [Publications] Y.Katsuragi,et al.: "Neutrophil functions in patients with severe periodontal disease." Adv.Dent.Res.2. 359-363 (1988)

    • Related Report
      1990 Annual Research Report
  • [Publications] S.Takashiba,et al.: "Polymorphism of compromised hosts in periodontitis patients." Recent Advances in Clinical Periodontology. 383-386 (1988)

    • Related Report
      1990 Annual Research Report
  • [Publications] Y.Mizushima,et al.: "Serum immunoglobulin G antibody in response to change of periodontal microflora." Recent Advances in Clinical Periodontology. 673-676 (1988)

    • Related Report
      1990 Annual Research Report
  • [Publications] S.Kokeguchi,et al.: "Cell surface protein antigen from <Wolinella>___ー <recta>___ー ATCC 33238." J.Clin.Microbiol.27. 1210-1217 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] 小林 充治: "好中球細胞膜糖蛋白(LFAー1ファミリ-)が関与する歯周病の病態研究" 日歯周誌. 32. 370-385 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 高柴 正悟: "ヒト白血球抗原(HLA)遺伝子の制限断片長多型(RFLP)解析による歯周病病態の分子生物学的研究" 日歯周誌. 32. 386-401 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 村山 洋二: "歯周病の診断と治療のための宿主防御細胞機能" 日本歯科評論. 568. 77-85 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 村山 洋二: "歯周疾患と生体防御" 歯科ジャ-ナル. 31. 793-805 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 小林 充治,村山 洋二: "好中球膜糖蛋白を“リスク因子"とする歯周病症例" 歯科ジャ-ナル. 31. 843-850 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] F.Nishimura,et al.: "A family study of a mother and daughter with increased susceptibility to earlyーonset periodontitis:microbiological,host defensive,and genetic analyses." J.Periodontol.61. 755-765 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] S.Kokeguchi,et al.: "Isolation and partial charcterization of a 39kDa major outer membrance protein of <Actinobacillus actinoーmycetemcomitans>___ー Y4." FEMS Microbiology Letters.77. 85-90 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] S.Kokeguchi,et al.: "Purification and characterization of two major outer membrance proteins from <Bacteroides gingivalis>___ー." Dentistry in Japan. 27. 1-6 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] M.Miyamoto,et al.: "Molecular cloning and sequence analysis of the 53ーkilodalton antigen of <Treponema denticola>___ー." Infect.Immun.

    • Related Report
      1990 Annual Research Report
  • [Publications] H.Kurihara,et al.: "Humoral immune response to an antigen from <Porphyromonas>___ー <gingivalis>___ー 381 in periodontal disease." Infect.Immun.

    • Related Report
      1990 Annual Research Report
  • [Publications] 阿久津 功: "歯周病の発症と進行におけるT細胞の関わりについての研究" 日歯周誌. 33. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] M.Kobayashi: "A molecular marker on neutrophil cell surface for disease susceptibility in a LJP patient." International Symposium of the Royal Society of Medicine,Program. 21 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] S.Takashiba: "Approach to gene diagnosis of patients with periodontal disease" J.Dent.Res.68. 267 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 明貝文夫: "若年性歯周炎を発症する1家族の症例研究" 日歯保誌. 32. 1740 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 村山洋二: "歯周病の診断と治療のための宿主防御細胞機能" 日本歯科評論. 568. 77-85 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Takashiba,S.: JADR(36th Annual Meeting,Abstract). 80 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 小林充治: 日歯周誌. 30. 112 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Takasiba,T.: "Recent Advances in Clinical Periodontology" Elsevier Science Publishers B.V., 383-386 (1988)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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