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Preliminary study for developing a new approach to treating malignant salivary gland tumors

Research Project

Project/Area Number 63480443
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 外科・放射線系歯学
Research InstitutionOsaka University

Principal Investigator

SHIRASUNA Kanemitsu  Osaka University School of Dentistry, The First Department of Oral and Maxillofacial Surgery, Associate Professor, 歯学部, 助教授 (30093420)

Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1989: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1988: ¥5,500,000 (Direct Cost: ¥5,500,000)
KeywordsSalivary gland / Salivary neoplasms / Cytodifferentiation / Cell growth / Growth factors / Induction of differentiation / Cancer therapy / 細胞増殖と細胞分化 / 増殖因子 / 増殖因子・分化誘導
Research Abstract

Since malignant salivary gland tumors are resistance to radiotherapy and/or chemotherapy, the development of new effective treatment is required. HSG cell line of human salivary gland origin may serve as useful tool for studying the biology of the salivary neoplasms and developing a new therapy. The HSG cells have a fine structure similar to intercalated duct cells and the inoculation of the cells into athymic mice results in production of adenocarcinoma. Cells with various phenotypes including myoepithelial-like cells could be isolated from HSG by repeated single cell cloning, changes of cultured conditions or treatment with certain differentiation inducers, such as dibutyryl cyclic AMP and glucocorticoid, suggesting that HSG is pluripotential in differentiation. Induction of more differentiated stages in HSG resulted in slowing of the growth. Inhibition of tumor growth associated with cytodifferentiation is also found in vivo.
Investigation of HSG in serum free medium suggested that this cell line produce EGF and beta-TGF which are probably involved in growth mechanism as regulator. Moreover, it was found that normal human fibroblasts exert a profound influence on the growth and differentiation of HSG, by an indirect effect involving diffusible factor(s). Partial purification and characterization of the factor(s) produced by the fibroblasts suggests that it may be a novel protein carrying both tumor inhibiting and differentiation inducing activities. These results indicate that induction of cytodifferentiation in HSG is possible, suggesting a therapeutic implication for salivary gland tumors. In order to establish cancer therapy, however, further investigation of molecular mechanism on growth and differentiation regulation of HSG as well as other cell lines of salivary gland tumors is required. Such studies are presently in progress using HSG and new cell lines recently established from adenoid cystic carcinoma.

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Kanemitsu SHIRASUNA et al.: "Isolation and characterization of different clones including myoepi-thelial-like variants from a clonal neopastic epithelial duct cell lines of human salivary gland origin" Cancer Research. 46. 1418-1426 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Setsuko HATAKEYAMA et al.: "Glucocorticoid-induced growth inhibition of human neoplastic salivary gland duct cell line" Acta Pathol.Jpn. 37. 587-595 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kanemitsu SHIRASUNA et al.: "Growth inhibition and differentiation of human salivary adenocarcionoma cells by medium conditioned with normal human fibroblasts" Cancer Reseach. 48. 2819-2824 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Setsuko HATAKEYAMA et al.: "Glucocorticoid-induced growth inhibition with enhanced expression of ductal epithelium of human salivary gland adenocarcinoma cells transplanted into athymic nude mice" Cancer. 62. 716-722 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kanemitsu SHIRASUNA et al.: "Different contents of glycosaminoglycans in a human neoplastic salivary duct cell line and its subclone with myoepithelial phenotype" Virchows Archiv B Cell Pathology. 57. 175-180 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kanemitsu SHIRASUNA et al.: "Bilolgical characterization of pseudocyst forming cell lines from adenoid cystic carcinoma of minor salivary gland origin" Cancer Research(in press). (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Shirasuna K., Watatani K., Sugiyama M., Morioka, S., and Miyazaki, T.: "Isolation and characterization of different clones including myoepithelial-like variants from a clonal neoplastic epithelial duct cell line of human salivary gland origin." Cancer Res., 46: 1418-1426, 1986.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Shirasuna K., Morioka S., Watatani K., Sugiyama M.: "Different expression of alkaline phosphatase in subclones of human neoplastic salivary duct cell line." Biochem. Biophys. Res. Comm., 138: 625-630, 1986.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Hatakeyama S., Kurokawa R., Satoh M., Suzuki, A., Ota, M. and Shirasuna K.: "Glucocorticoid-induced growth inhibition of human neoplastic salivary gland duct cell line (HSG)." Acta Pathol. Jpn., 37: 587-595, 1987.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Shirasuna K.: "Experimental approach to therapy of salivary gland neoplasms" J. Jpn Stomatol. Soc. 36: 982-1015, 1987.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Shirasuna K., Morioka S., Watatani K., Hayashido Y., Furusawa H., Sugiyama M., Okura M., and Matsuya T.: "Growth inhibition and differentiation of human salivary adenocarcinoma cells by conditioned medium with normal human fibroblasts." Cancer Res., 48: 2819-2824, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Hatakeyama S., Sashima M., Shirasuna K., Satoh, M., and Suzuki A.: "Glucocorticoid-induced growth inhibition with the enhanced expression of ductal epithelium of human salivary gland adenocarcinoma cells transplanted into athymic nude mice." Cancer (Phila.), 62: 716-722, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Shirasuna K., Furusawa H., Morioka S., Watatani K., and Matsuya T.: "Different contents of glycosaminoglycans in a human neoplastic salivary duct cell line and its subclone with a myoepithelial phenotype." Virchows Arch. Cell Pathol., 57: 175-180, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Shirasuna K., Watatani K., Furusawa H., Saka M., Morioka S., Yoshioka H. and Matsuya T.: "Biological characterization of pseudocyst-forming cell lines from adenoid cystic carcinomas of minor salivary gland origin." Cancer Res., 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kanemitsu SHIRASUNA et al: "Growth inhibition and differentiation of human salivary adenocarcinoma cells by medium conditioned with normal human fibroblasts" Cancer Research. 48. 2819-2824 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Setsuko HATAKEYAMA et al: "Glucocorticoid-induced growth inhibition with enhanced expression of ductal epithelium of human salivary gland adenocarcinoma cells transplanted into athymic nude mice" Cancer. 62. 716-722 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] 古沢栄之 他: "唾液腺癌細胞の産生するグリコサミノグリカン" 大阪大学歯学雑誌. 33. 462-467 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kanemitsu SHIRASUNA et al: "Different contents of glycosaminoglycans in a human reoplastic salivary duct cell live and its subclone with myoepithelicl phenotype" Virchows Archiv B Cell Pathology. 57. 175-180 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 綿谷和也 他: "ヒト腺様嚢胞癌培養細胞の分離" 日本口腔科学会雑誌. 38. 898-904 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kanemitsu SHIRASUNA et al: "Biological characterization of pseudocyst forming cell lines from adenoid cystic earcinoma of minor salivary gland origin" Cancer Research. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kanemitsu SHIRASUNA: Cancer Research. 48. 2819-2824 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Setsuko HATAKEYAMA: Cancer. 62. 716-722 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kanemitsu SHIRASUNA: Virchows Archiv B Cell Pathology. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 古沢栄之: 大阪大学歯学雑誌. 33. 462-467 (1988)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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