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Studies of IFN, IL-1, and Tnf on Induction of Differentiation and Activation of Neutrophils.

Research Project

Project/Area Number 63480448
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 外科・放射線系歯学
Research InstitutionShowa University

Principal Investigator

NAGUMO Masao  Showa University, School of Dentistry Professor, 歯学部, 教授 (70013993)

Co-Investigator(Kenkyū-buntansha) OSHIMA Osamu  Showa University, School of Dentistry assistant professor, 歯学部, 講師 (50167300)
KAKUTA Saburo  Showa University, School of Dentistry associate professor, 歯学部, 助教授 (40112726)
桜田 重世  昭和大学, 歯学部, 講師 (00112730)
Project Period (FY) 1988 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥5,700,000 (Direct Cost: ¥5,700,000)
Fiscal Year 1990: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1989: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1988: ¥5,200,000 (Direct Cost: ¥5,200,000)
KeywordsIFN / TNF / TGF-beta / Differentiation of Leukemia cell lines / Synergistical effect / myc mRNA / Activation of neutrophils / Ca^<++> influx / 白血病細胞 / 分化誘導作用 / 末梢血好中球 / 活性化 / IFNーγ / カルシウムイオン / 好〓球活性化 / 分化誘導 / INF / IL-1 / 好中球 / 活性化の機序
Research Abstract

The experiments have been mainly undertaken to examine the effects of IFN and TNF on proliferation and differentiation of leukemia cell lines, and the effects of these cytokines on the activation of human peripheral neutrophils. Results obtained are as follows :
1. The effects of IFN and TNE on proliferation of human leukemia cell lines.
(1) Proliferation of human leukemia cell lines (ML-1, U-937) was inhibited by IFN and TNF.
(2) IFN and TNF induced differentiation of human leukemia cell lines, respectively.
(3) Differentiation of human leukemia cell lines was additively or synergistically induced by combination of TNF and TGF-beta. However, change of TNF receptors was not observed.
(4) In U-937 cells, either TNF and TGF-beta alone decreased myc mRNA expression within one hour. When combined with TGF-beta, TNF additively or synergistically decreased myc mRNA expression.
2. The effects of IFN, IL-1, and TNF on peripheral neutrophil functions. The effects of cytokines (IFN-betaandgamma, IL-1alphaandbeta, IL-2, TNF, G-CSF) on the generation of superoxide (O^-_) of human neutrophils stimulated by PMA or opsonized zymosan were investigated to examine the activation activities of these cytokines.
(1) IFN-gamma, IL-1, TNF and G-CSF enhanced the production of O^-_ form human peripheral neutrophils and INF-gamma was most effective. However, IFN-rho and IL-2 showed no effect.
(2) The mechanism of enhancement of neutrophil functions was investigated by using IFN-gamma as a cytokine and it is suggested that influx of Ca^<++> into cytoplasma may be important to activate neutrophil functions.

Report

(4 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • Research Products

    (35 results)

All Other

All Publications (35 results)

  • [Publications] 武田 健: "インタ-フエロンーγによるヒト骨髄性白血病細胞の分化誘導とBRMによるその作用の増強" Biotherapy. 2. 66-70 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 佐藤 篤: "好中球の活性酸素産生に及ぼすbloomycinの効果" 日本口腔外科学会雑誌. 34. 1852-1856 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ryutaro Kamijyo: "Suppresion of TNFーstimulated proliferation of diploid fibroblasts and TNFーinduce cytotoxicity against tranceformed fibroblasts by TGFー." BBRC. 155. 155-162 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 上條 竜太郎: "TNF変異体による顕粒球活性化" 炎症. 9. 25-28 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 桜田 重世: "局所麻酔剤の好中球機能に及ぼす影響について" 日本口腔外科学会雑誌. 35. 267-270 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ryutaro Kamijyo: "Induction of differentiation of human monoblastic and myeloblastic leukemia cell lines by TNF muteins." BBRC. 160. 820-827 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ryutaro Kamijyo: "Effects of combinations of transforming growth factorand induction of differentiation of human myelogenous leukemic cell lines." Journal of Immunology. 144. 1311-1316 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 上条 竜太郎: "TGFーβの線維芽細胞に対する作用の抑制" 炎症. 10. 369-373 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sanju Iwamoto: "Induction of resistance to TNF cytotoxicity and mitochodral superoxide dismutase on Uー937 cells by 1,25ーdihydroxyvitamin D3." Biochemical and Biophysical Research Communications. 170. 73-79 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yudari Gogun: "Enhancement of superoxide dismutase activty in cancer cell lines by anticancer drugs." Journal of Clinical Biochemistry and Nutrition. 8. 85-92 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 堀 滋: "インタ-フエロンーγによる好中球ス-パ-オキサイド産生の増強とその作用機序" 炎症. 11. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ken Takeda: "Differentiation induction activity of interferon- and its enhancement in combination with biological response modifiers" Biotherapy. 2. 66-70 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Atushi Sato: "Effect of bleomycin on the generation of oxygen species in human neutrophils" Jpn. J. Oral Maxillofac. Surg.34. 28-32 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ryutaro Kamijo: "Suppression of TNF-stimulated proliferation of diploid fibroblasts and TNF- induced cytotoxicity against transformed fibroblasts by TGF-" BBRC. 158. 155-162 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ryutaro Kamijo: "Stimulation of polymorphonuclear neutrophils by TNF muteins" Jpn. J. Inflammation. 9. 25-28 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shigeyo Sakurada: "Effects of local anesthetics on neutrophil functions" Jpn. J. Oral Maxillofac. Surg.35. 267-270 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ryutaro Kamijo: "Induction of differentiation of human monoblastic and myeloblastic leukemia cell lines by TNF muteins" BBRC. 160. 820-827 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ryutaro Kamijo: "Effects of combinations of transforming growth factor-beta, and tumor necrosis factor on induction of differentiation of human myelogenous leukemic cell lines" J. Immunology. 144. 1311-1316 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sanju Iwamoto: "Induction of resistence to TNF cytotoxicity and mitochondrial superoxide dismutase on U-937 cells by 1, 25-dihidroxyvitamin D_3" BBRC. 170. 73-79 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ryutaro Kamijo: "Effects of TGF-beta on TNF actions against fibroblasts and granulocytes" Jpn. J. Inflammation. 10. 369-373 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yukari Gogun: "Enhancement of superoxide dismutase activity in cancer cell lines by anticancer drugs" J. Clin. Biochem. Nutr.8. 85-92 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shigeru Hori: "Enhancement of superoxide production by interferon-gamma on human neutrophils" Jpn. J. Inflammation.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 上条 竜太郎: "TGFーβの線維芽細胞に対する作用の抑制" 炎症. 10. 369-373 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Sanju Iwamoto: "Induction of resistance to TNF cytotoxicity and mitochodral superoxide dismutase on Uー937 cells by 1,25ーdihydroxyvitamin D3." Biochemical and Biophysical Research Communications. 170. 73-79 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yukari Gogun: "Enhancement of superoxide dismutase activity in cancer cell lines by anticancer drugs." Journal of Clinical Biochemistry and Nutrition. 8. 85-92 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 堀 滋: "インタ-フェロンーγによる好中球ス-パ-オキサイド産生の増強とその作用機序" 炎症. 11. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 南雲 正男: "テラルビシンによって重篤な顆粒球減少症をきたした上顎癌の1例" 日本口腔外科学会雑誌.

    • Related Report
      1990 Annual Research Report
  • [Publications] Ryutaro Kamijyo: "Effects of TGFーβ combined with TNF or IFNーγ on differentiation,proliferation and expression of myc mRNA in human myelogeneous leukemia cell lines." Journal of Immunology.

    • Related Report
      1990 Annual Research Report
  • [Publications] Ryutaro Kamijo: "Induction of Differentiation of Human Monoblaslic and Myeloblastic heukemia Cell Lines by TNF Muteins" Biochemical and Biophysical Research Communication. 160. 820-827 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Ryutaro kamijo: "Effects of Combinations of Transforming Growth Factorーβ and TNF on Induction of Differentiation of Human Myelogenous heukemic Cell Lines." Journal of Immunology. 144. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 上条竜太郎: "TGFーβによるTNFの線維芽細胞に対する作用の抑制" 炎症. 10. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 南雲正男: "INFーγによる好〓球の活性化とその作用機序" 炎症. 10. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 上条竜太郎: 炎症. 9(1). inpress (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Ryutaro Kamijo: Biochemical and Biophysical Research Communications.158(1). 155-162 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 南雲正男: 炎症. 9. (1989)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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