| Project/Area Number |
63480509
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
分子遺伝学・分子生理学
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| Research Institution | Inst. of Applied Microbiol. University of Tokyo |
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Principal Investigator |
OISHI Michio Inst. of Applied Microbiol. Univ. of Tokyo, Professor, 応用微生物研究所, 教授 (00126004)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Toshio Inst. of Applied Microbiol. Univ. of Tokyo, Assistant, 応用微生物研究所, 助手 (60201208)
NOMURA Shintaro Inst. of Applied Microbiol. Univ. of Tokyo, Assistant, 応用微生物研究所, 助手 (80159087)
AYUSAWA Dai Inst. of Applied Microbiol. Univ. of Tokyo, Associate Professor, 応用微生物研究所, 助教授 (00142109)
西森 克彦 東北大学, 農学部, 助教授 (10164609)
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| Project Period (FY) |
1988 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1989: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1988: ¥4,900,000 (Direct Cost: ¥4,900,000)
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| Keywords | Differentiation / Embryonal / Phosphorylation / erythroid / 分化 / DIF-III / herbimycin A / genistein / ST 638 / DIF-I / DIF-II / 分化決定因子(commitment factor) |
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| Research Abstract |
We have found that herbimycin A is an effective inducing agent capable of triggering differentiation in two typical mouse in vitro differentiation systems, which have been considered to be quite different in their mechanism of induction: endoderm differentiation of embryonal carcinoma (F9) cells and terminal erythroid differentiation of (MEL) cells. The results suggest that there is a common step in the intracellular differentiation cascade which is, directly or indirectly, associated with phosphorylation at specific (tyrosine) residues of cellular proteins.
An erythroid-inducing activity was detected in cell-free extracts from MEL cells which had been incubated with dimethyl sulfoxide or hexamehylenebisacetamide for a prolonged period of time. The newly detected activity was apparently different from the previously reported differentiation-inducing factors and seemed to be derived from a proeinacious factor in the cytoplasm. The timing of the appearance of the activity in the extracts coincided with that of cellular commitment to differentiation. The activity was not induced in a differentiation defective MEL cell line and the induction of the activity was inhabited by PMA, a specific inhibitor for MEL cell differentiation.
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