• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Regulation of Ethylene Biosynthesis --- Mechanism for SAM-Induced Inactivation of ACC synthase

Research Project

Project/Area Number 63540524
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 植物生理学
Research InstitutionTohoku University

Principal Investigator

SATOH Shigeru  College for General Education Department of Biological Sciences, Associate Professor., 教養部, 助教授 (40108428)

Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1989: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1988: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywords1-aminocyclopropane-1-carboxylic acid / S-adenosylmethionine / ethylene biosynthesis / mechanism-based inactivation / L-vinylglycine / 1-アミノシクロプロパ-1-カルボン酸合成酵素 / 酵素不活性化反応 / 1-アミノシクロプロパン-1-カルボン酸合成酵素 / S-アデノシル-1-メチオニン / エチレン生成
Research Abstract

It was investigated the mechanism for the inactivation of 1-aminocyclopropane -1-carboxylate (ACC) synthase by the substrate, S-adenonosylmethionine (SAM), using an enzyme preparation partially purified from tomato ( Lycopersicon esculentum Mill.) fruits, and the following results were obtained.
(1) SAM molecule has two sulfonium diastereomers, (+)- and (-)-SAM. When their activities as an inactivator of ACC synthase were compared, it was demonstrated that (+)-SAM inactivated the enzyme three times faster than (-)-SAM.
(2) The contents of both (+)- and (-)-SAM were examined in some plant tissues, which produced ethylene at high rates. They contained (-)-SAM, but not (+)-SAM. Thus the natural (-)-SAM was thought to be responsible for the auto- inactivation process of the enzyme in vivo.
(3) The enzyme was incubated with ^<14>C-SAM labeled at different positions, and the radioactivity incorporation into the enzyme protein was analyzed. It was revealed that 2-aminobutyric acid portion of SAM is linked covalently into ACC synthase.
(4) L-Vinylglycine was shown to act as an irreversible inactivator of ACC synthase in the presence of pyridoxal phosphate. This finding coupled with that described in (3) suggested that the inactivation of ACC synthase by SAM proceeds through the formation of a vinylglycine-ACC synthase complex as an intermediate. A scheme for the mechanism-based inactivation of ACC synthase was postulated.
(5) The specific labeling of ACC synthase by ^<14>C-SAM would facilitate the isolation of radio-labeled active-site peptide of ACC synthase and the subsequent determination of its amino-acid sequence.

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Shigeru Satoh and Shang Fa Yang: "Specificity of S-adenosyl-L-methionine in the inactivation and the labeling of 1-aminocyclopropane-1-carboxylate synthase isolated from tomato fruits" Archives of Biochemistry and Biophysics. 271. 107-112 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Shigeru Satoh and Shang Fa Yang: "Inactivation of 1-aminocyclopropane-1-carboxylate synthase by L-vinylglycine as related to the mechanism-based inactivation of the enzyme by S-adenosyl-L-methionine" Plant Physiology. 91. 1036-1039 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 佐藤茂: "ACC合成酵素の不活性化によるエチレン生合成の調節" 植物の化学調節. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Shigeru Satoh and Shang Fa Yang(分担執筆,pp41ー48): "H.Clijsters et al.(eds.),Biochemical and Physiological Aspects of Ethylene Production in Lower and Hugher Plants" Kluwer Academic Publishers, 354 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Shigeru Satoh and Shang Fa Yang.: "Specificity of S-adenosyl-L-methionine and the labeling of 1-aminocyclopropane-1-carboxylate synthase isolated from tomato fruits." Archives of Biochemistry and Biophysics 271:107-112 (1989).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Shigeru Satoh and Shang Fa Yang.: "Inactivation of 1-aminocyclopropane-1-carboxylate synthase by L-vinylglycine as related to the mechanism-based inactivation of the enzyme by S-adenosyl-L-methionine." Plant Physiology 91:1036-1039 (1989).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Shigeru Satoh.: "Regulation of ethylene biosynthesis by 1-aminocyclopropane-1-carboxylate synthase inactivation." Chemical Regulation of Plants 25.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Shigeru Satoh and Shang Fa Yang.: S-Adenosylmethionine-dependent inactivation of 1-aminocyclopropane-1-carboxylate synthase isolated from tomato fruits. In H. Clijsters, M. De Proft, R. Marcelle and M. Van Poucke (eds.) Biochemical and Physiological Aspects of Ethylene Production in Lower and Higher Plants.Kluwer Academic Publishers, Dordrecht, The Netherlands, 41-48 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Shigeru Satoh and Shang Fa Yang: "Specificity of S-andnosyl-L-methionine in the inactivation and the labeling of 1-aminocyclopropane-1-carboxylate synthase isolated from tomato fruits" Archives of Biochemistry and Biophysics. 271. 107-112 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Shigeru Satoh and Shang Fa Yang: "Inactivation of 1-aminocyclopropane-1-carboxylate synthase by L-vinylglycine as related to the mechanism-based inactivation of the enzyme by S-adenosyl-L-methionine" Plant Physiology. 91. 1036-1039 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 佐藤茂: "ACC合成酵素の不活性化によるエチレン生合成の調節" 植物の化学調節. 25. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Shigeru,Satoh;Shang,Fa Yang: Archives of Biophysics.

    • Related Report
      1988 Annual Research Report
  • [Publications] Shigeru,Satoh: Journal of Experimental Botany.

    • Related Report
      1988 Annual Research Report
  • [Publications] Shigeru,Sato;Shang,Fa Yang: Plant physiology.

    • Related Report
      1988 Annual Research Report

URL: 

Published: 1988-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi