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Studies on the Elicitation of Stress Compounds in Plant Cells

Research Project

Project/Area Number 63540529
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 植物生理学
Research InstitutionToyama Medical and Pharmaceutical University

Principal Investigator

NISHI Arasuke  Toyama Med. & Pharmaceut. University, Fac. of Pharmaceut. Sci., Professor, 薬学部, 教授 (50019098)

Project Period (FY) 1988 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1989: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1988: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsStress compound / Carrot / Elicitor / Phytoalexin / Chitinase / bateー1, 3-Glucanase / 6ーメトキシメレイン / βー1,3ーグルカナ-ゼ / 6-メトキシメレイン / β-1,3-グルカナ-ゼ / キチナーゼ / エリシター
Research Abstract

Carrot cells accumulate isocoumarin phytoalexin 6-methoxymellein when they are challenged by microorganisms. The synthesis of this compound was also elicited by oligouronides or ethylene. Cell-free extract from elicitor-treated carrot roots catalyzed NADPH-dependent synthesis of 6-hydroxyーmellein from acetyl-CoA and malonyl-CoA which was further converted to 6-methoxymellein by a specific O-methyltransferase. In the absence of NADPH, triacetic acid lactone was produced as a derailment product of the reaction. This finding suggests that the NADPH-dependent reduction takes place at the triacetate stage before the condensation of the third malonyl-CoA. Besides phytoalexin, carrot cells produced chitinasa sa a response to fungal infection. This process was elicited by various fungal components and also by ethylene. Cells accumulated many isoforms of the enzyme according to the elicitor applied to them. All the enzymes induced by various fungal components were found to be endoーchitinase while ethylene induced the production of exoーhydrolases which released chitobiose from insoluble chitin. Carrot beta-1,3-glucanse was mainly localized in cellーwall fraction. The enzymes were purified to apparent homoginity and characterized. The carrot cells contained 2 isoenzymes : both of which were glycoproteins with a similar molecular size. Unlike tobacco plants, however, teh beta-1,3-glucanase in carrot cells seem to be constitutive and was not affected by various external stresses.

Report

(4 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Fumiya Kurosaki: "Biosynthesis of dihydroisocoumarin by extracts of elicitorーtreated carrot root" Phytochemistry. 28. 1843-1845 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Fumiya Kurosaki: "Chitinase isoenzymes induced in carrot cell cultures by treatment with ethylene." Phytochemistry. 28. 2989-2992 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Fumiya Kurosaki: "Derailment product in NADPHーdependent synthesis of a dihydroisocoumarin 6ーhydroxymellein by elicitortreted carrot cell extracts." Eur.J.Biochem.185. 85-89 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Fumiya Kurosaki: "Localization and secretion ofβー1,3ーglucanase of cultured carrot cells." Plant Sci.65. 39-43 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Fumiya Kurosaki: "Chitinase induction in carrot cell cultures treated with various fungal components" Biochem.Intern.20. 99-106 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Fumiya Kurosaki: "Purification and characterization of wallーboundβー1,3ーglucanase in cultured carrot cells"

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Fumiya Kurosaki: "Role of βー1,3ーglucanase in defense responses of cultured carrot cells."

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Fumiya Kurosaki, Yuji Kizawa and Arasuke Nishi: "Biosynthesis of dihydroisocoumarin by extracts of elicitor-treated carrot root." Phytochemistry. 28. 1843-1845 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Fumiya Kurosaki, Nobuhiko Tashiro, Reiko Gamou and Arasuke Nishi: "Chitinase isoenzymes induced in carrot cell culture by treatment with ethylene." Phytochemistry. 28. 2989-2992 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Fumiya Kurosaki, Yuji Kizawa and Arasuke Nishi: "Derailment product in NADPH-dependent synthesis of a dihydroisocoumarin 6-hydroxymellein by elicitorーtreated carrot cell extracts." Eur. J. Biochem.185. 85-89 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Fumiya Kurosaki, Yasuhiro Tokitoh, Masashi Morita and Arasuke Nishi: "Localization and secretion of beta-1, 3-glucanase of cultured carrot cells." Plant Sci.65. 39-43 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Fumiya Kuroski, Nobuhiko Tasiro and Arasuke Nishi: "Chitinase induction in carrot cell cultures treated with various fungal components." Biochem. Intern.20. 99-106 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Fumiya Kurosaki, Yasuhiro Tokitoh and Arasuke Nishi: "Purification and characterization of wallーbound betaー1, 3-glucanase in cultured carrot cells."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Fumiya Kurosaki, Yasuhito Tokitoh and Arasuke Nishi: "Role of bate-1, 3-glucanase in defense responses of cultured carrot cells."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Fumiya Kurosaki: "Purification and characterization of wallーbound βー1,3ーglucanase in cultured carrot cells."

    • Related Report
      1990 Annual Research Report
  • [Publications] Fumiya Kurosaki: "Role of βー1,3ーglucanase in defense responses of cultured carrot cells."

    • Related Report
      1990 Annual Research Report
  • [Publications] Fumiya Kurosaki: "Biosynthesis of dihydroisocoumarin by extracts of elicitor-treated carrot root" Phytochemistry. 28. 1843-1845 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Fumiya Kurosaki: "Chitinase isoenzymes induced in carrot cell culture by reatment with ethylene" Phytochemistry. 28. 2989-2992 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Fumiya Kurosaki: "Derailment product in NADPH-dependent synthesis of a dihydroisocoumarin 6-hydroxymellein by elicitor-treated carrot cell extract" Eur.J.Biochem.185. 85-89 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Fumiya Kurosaki: "Localization and secretion of β-1,3-glucanase of cultured carrot cells" Plant Science. 65. 39-43 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Fumiya Kurosaki: "Chitinase induction in carrot cell cultures treated with various fungal components" Biochem.International.

    • Related Report
      1989 Annual Research Report
  • [Publications] Fumiya,Kurosaki: Phytochemistry. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Fumiya,Kurosaki: Plant and Cell Physiology.

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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