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Cyclic Nucleotide-Sensitive Ion Channels In Olfactory Receptor Cell Membrane.

Research Project

Project/Area Number 63540557
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 動物発生・生理学
Research InstitutionHokkaido University

Principal Investigator

SUZUKI Noriyo  Hokkaido University, Faculty of Science, Lecturer, 理学部, 講師 (10001851)

Co-Investigator(Kenkyū-buntansha) SHINOZAWA Takao  Gunma University, Faculty of Technology, Associate Professor, 工学部, 助教授 (30025449)
Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1989: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1988: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsOlfactory Transduction Mechanism / Cyclic Nucleotide-Sensitive Ion channels / Dynamics of Ion Channel Activity / Cation Selectivity / Unit Conductance / Cation Channels / Purification of Olfactory Cilia / Ion Channel Protein / 嗅受容機構 / 細胞内伝達 / 環状ヌクレオチド感受性イオンチャンネル / パッチクランプ / 単位コンダクタンス / 嗅繊毛膜の分離 / イオンチャンネル蛋白分子の同定
Research Abstract

It has been proposed that an adenylate or guanylate cyclase cascade is involved in coupling odorant-activation of olfactory receptor molecule with conductance changes, giving rise to olfactory receptor potentials. Cyclic nucleotides have been thought to act on the conductance as intracellular messengers. Although the odorant-activation of adenylate cyclase has been examined extensively, molecular nature of the proteins in the cascade and the mechanism how these as a whole regulate the conductance have not been fully understood. We have focused on the studies on electrical properties and molecular nature of the cyclic nucleotide-activated ion channels, and have found that the channels have the following characteristics: (1)The channels are activated directly by both cyclic AMP and cyclic GMP. This indicates that the phosphorlylation of channel molecule is not required in its activation. There is a possibility whether olfactory adenylate cyclase is not a single species of enzyme or does … More not have a substrate specificity for production of different cyclic nucleotides. (2)The channels have a low cationic selectivity. Many monovalent and divalent cations can pass through the channels. Thus, the channels may be categorized into a species of the "cation channels". (3)The channel molecule has at least two cooperative binding sites for the cyclic nucleotides and their affinity (K_<1/2>) to the cyclic nucleotides ranges between 3 and 5 uM. (4)The channels have a unit conductance of 24 pS. The distribution of channel open times is expressed as a sum of two exponentials. The time constants are 10.1 msec and 0.9 msec at 1 uM cAMP. The channels are blocked by divalent cations likely in a voltage-dependent manner. This raises the possibility that divalent cations play roles in the adaptation process through whether their direct blockage of the channels or their activation or inhibition of phosphodiesterase dr adenylate cyclase activity. (5)The channels are distributed in not only olfactory cilia but also dendrite and soma membrane. (6)The candidate channel molecule is a protein with a molecular mass of 35-39 kDa. Less

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] 篠沢隆雄: "脊椎動物の視細胞と嗅細胞における環状ヌクレオチドによるイオンチャンネルの直接開閉-蛋白質のリン酸化を介さない環状ヌクレオチドの生理機能-" 生物物理. 28. 38-41 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Fukunaga,S.: "Chemotaxis for methamphetamine in Escherichia coli." Microbiol.Immunol.32. 115-117 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Suzuki,N.: "Role of external calcium ions in adaptation process of cyclic nucleotide-induced current in isolated olfactory receptor cells." Proc.Jap.Symp.Taste & Smell. 22. 189-192 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Suzuki,N.: "External calcium effect on adaptation process of cyclic nucleotide-gated current in solitary olfactory receptor cells." Chemical Senses. 14. 318 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Suzuki,N.: "Properties of single cyclic nucleotide-actived ion channels in the olfactory receptor soma membrane." Proc.Jap.Symp.Taste & Smell. 23. 71-74 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Suzuki,N.: "Single cyclic nucleotide-activated ion channel activity in olfactory receptor cell soma membrane." Neurosci.Res.Supl.11. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Suzuki,N.(Ed.I.J.Miller,Jr.): "Cyclic nucleotide-induced conductance increase in solitary olfactory receptor cells.In:The Beidler Symposium On Taste And Smell" Book Service Assoc.,Inc.Winston-Salemn, 8 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Suzuki,N.(Eds.J.G.Brand et al.): "Chapter 23.Voltage-and cyclic nucleotide-gated currents in isolated olfactory receptor cells.In:Chemical Senses Volume 1,Receptor Events and Transduction in Taste and Olfaction." Marcel Dekker,Inc.New York and Basel, 24 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Shinozawa, T.: "Direct activation by cyclic nucleotides of ion channels in visual and olfactory receptor cells - Physiological function of cyclic nucleotides for ion channel activation through non-phosphorlylation process." Biophysics 28:38-41, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Fukunaga, S.: "Chemotaxis for methamphetamine in Escherichia coli." Microbiol. Immunol. 32:115-117, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Suzuki, N.: "Role of external calcium ions in adaptation process of cyclic nucleotide-induced current in isolated olfactory receptor cells." Proc. Jap. Symp. Taste and Smell 22:189-192, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Suzuki, N.: "External calcium effect on adaptation process of cyclic nucleotide-gated current in solitary olfactory receptor cells." Chemical Senses 14:318, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Suzuki, N.: "Properties of single cyclic nucleotide-activated ion channels in the olfactory receptor soma membrane." Proc. Jap. Symp. Taste and Smell 23:71-74, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Suzuki, N.: "Single cyclic nucleotide-activated ion channel activity in olfactory receptor cell soma membrane." Neurosci. Res. Suppl. 11, 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Suzuki, N.: "Cyclic nucleotide-induced conductance increase in solitary olfactory receptor cells. Miller Jr., I.J.(Ed)" The Beidler Symposium On Taste And Smell., Book Service Assoc., Inc., Winston-Salemn, N. C., pp. 173-181, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Suzuki, N.: "Voltage- and cyclic nucleotide-gated currents in isolated olfactory receptor cells. Brand, J.G. et al.(Eds)" Chemical Senses Volume 1. Receptor Events and Transduction in Taste and Olfaction., Marcel Dekker, Inc., New York and Basel, pp. 469-493, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 篠沢隆雄: "脊椎動物の視細胞と嗅細胞における環状ヌクレオチドによるイオンチャンネルの直接開閉-蛋白質のリン酸化を介さない環状ヌクレオチドの生理機能-" 生物物理. 28. 38-41 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Fukunaga,S.: "Chemotaxis for methamphetamine in Escherichia coli." Microbiol.Immunol.32. 115-117 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Suzuki,N.: "Role of external calcium ions in adaptation process of cyclic nucleotide-induced current in isolated olfactory receptor cells." Proc.Jap.Symp.Taste & Smell. 22. 189-192 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Suzuki,N.: "External calcium effect on adaptation process of cyclic nucleotide-gated current in solitary olfactory receptor cells." Chemical Senses. 14. 318 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Suzuki,N.: "Properties of single cyclic nucleotide-activated ion channels in the olfactory receptor soma membrane." Proc.Jap.Symp.Taste & Smell. 23. 71-74 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Suzuki,N.: "Single cyclic nucleotide-activated ion channel activity in olfactory receptor cell soma membrane." Neurosci.Res.Supl.11. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Suzuki,N.(Ed.I.J.Miller,Jr.): "Cyclic nucleotide-induced conductance increase in solitary olfactory receptor cells.In: The Beidler Symposium On Taste And Smell" Book Service Assoc.,Inc.Winston-Salemn, 8 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Suzuki,N.(Eds.J.G.Brand et al.): "Chapter 23.Voltage-and cyclic nucleotide-gated currents in isolated olfactory receptor cells. In:Chemical Senses Volume 1,Receptor Events and Transduction in Taste and Olfaction." Marcel Dekker,Inc.New York and Basel, 24 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 篠沢隆雄: 生物物理. 28. 38-41 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Fukunaga,S.: Microbiol.immunol.32. 115-117 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Suzuki,N.: Chemical Senses. 13. 311 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Suzuki,N.: Proc.Jap.Symp.Taste & Smell. 22. 189-192 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Suzuki,N.: Chemical Senses. 14. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Suzuki,N.(Ed.I.J.Miller,Jr.): "Cyclic nucleotide-induced conductance increase in solitary olfactory receptor cells.In The Beidler Symposium on Taste And Smell" Book Service Assoc.,Inc.Winston-Salemn, 173-181 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Suzuki,N.(Eds.J.G.Brand et al.): "Chapter 23.Voltag-and cyclic nucleotide-gated currents in isolated olfactory receptor cells.In Chemical Senses: Molecular Aspects of Taste and Odor Reception." Marcel Dekker,Inc.New York, (1989)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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