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Development of the Mehtod for Separating and Determining a Small Amount of Protein by the Use of Chemiluminescence Reaction.

Research Project

Project/Area Number 63550567
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 工業分析化学
Research InstitutionDoshisha University

Principal Investigator

HARA Tadashi  Doshisha Univ.., Dep't of Eng., Professor, 工学部, 教授 (20066054)

Project Period (FY) 1988 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1990: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1989: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1988: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsProtein / Separation / Determination / Chemiluminescence / Detector / Immunosensor / Flow-injection analysis / Ion-exchange chromatography / キャピラリ-電気泳動 / 化学発光検出器 / 分離分析 / 検出 / トリプトファン / 5ーヒドロキシインド-ル酢酸 / 標識 / 化学発光反応 / 分離・定量法 / 液体クロマトグラフィ- / フローインジェクション分析 / 液体クロマトグラフィー
Research Abstract

The following results were obtained during 1988 - 1990,
1. Development of chemiluminescent detectors :
(1) The flow-injection analysis of protein using ultrasonic chemiluminescence of luminol.
Bull. Chem. Soc. Jpn., 61, 2996(1988).
(2) Improvement in FIA system for determining small amounts of proteins with chemiluminescence detection. BUNSEKI KAGAKU, 38, T100(1989).
2. Connection of a separation column for proteins with a chemiluminescence detector :
(1) High sensitive matal chelate affinity chromatography.
Bull. Chem. Soc. Jpn., 61, 2779(1988).
(2) A zeolite column-chemiluminescence detection system for the separation and the determination of a protein mixture.
Bull. Chem. Soc. Jpn., 62, 1501(1989).
(3) High-sensitive detection of proteins by an ion-exchange chromatograph-chemiluminescence detector system.
Bull. Chem. Soc. Jpn., 63, 770(1990).
3. Development of an immunosensor using chemiluminescence reaction :
(1) A highly sensitive fiber-optic immunosensor using a metal-complex compound as a chemiluminescent catalyst.
Bull. Chem. Soc. Jpn., 62, 2844(1989).
4. Miscellaneous :
(1) Experimental consideration on the chemiluminescence detection of protein using the 1,10-phenanthroline-hydrogen peroxide-copper (II) system.
Bull. Chem. Soc. Jpn., 63, 2423(1990).
(2) Chemiluminescence of L-tryptophan in copper (II) - hydrogen peroxide - potassium sodium tartrate system.
Chem. Lett., 843(1990).
(3) Chemiluminescence analyses of biological constituents using metal-complex catalysts. A review.
Anal. Sci., 6, 797(1990).
(4) Additional two papers in THE SCI. AND ENG. REVIEW OF DOSHISHA UNIV.

Report

(4 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Tadashi Hara et al.: "The Determination of a Small Amount of Biological Constituent by the Use of Chemiluminescence.XIV,The FlowーInjection Analysis of Protein Using Ultrasonic Chemiluminescence of Luminol." Bull.Chem.Soc.Jpn.61. 2996-2998 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tadashi Hara et al.: "Improvement in FIA system for determinint small amounts of proteins with chemiluminescence detection." BUNSEKI KAGAKU. 38. T100-T103 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tadashi Hara et al.: "The Determination of a Small Amount of Biological Constituent by the Use of Chemiluminescence. XIII. High Sensitive Metal Chelate Affinity Chromatography." Bull.Chem.Soc.Jpn.61. 2779-2783 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tadashi Hara et al.: "The Determination of a Small Amount of a Biological Constituent by the Use of Chemiluminescence.XV.A Zeolite ColumnーChemiluminescence Detection System for the Separation and the Determination of a Protein Mixture." Bull.Chem.Soc.Jpn.62. 1501-1508 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tadashi Hara et al.: "The Determination of a Small Amount of a Biological Constituent by Use of Chemiluminescence.XVI.HighーSensitive Detection of Proteins by an IonーExchange ChromatographーChemiluminescence Detector System." Bull.Chem.Soc.Jpn.63. 770-776 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tadashi Hara et al.: "A Highly Sensitive FiberーOptic Immunosensor Using a MetalーComplex Compound as a Chemiluminescent Catqlyst." Bull.Chem.Soc.Jpn.62. 2844-2848 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tadashi Hara et al.: "The Determination of a Small Amount of a Biological Constituent by the Use of Chemiluminescence.XVII.Experimental Consideration on the Chemiluminescence Detection of Protein Using the 1,10ーPhenanーthrolineーHydrogen PeroxideーCopper(II)system." Bull.Chem.Soc.Jpn.63. 2423-2425 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tadashi Hara et al.: "Chemiluminescence of Lーtryptophan in Copper(II)ーHydrogen PeroxideーPotassium Sodium Tartrate System." Chem.Lett.ーー. 843-846 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tadashi Hara et al.: "Chemiluminescence Analyses of Biological Constituents Using MetalーComplex Catalysts.A Review." Anal. Sci.6. 797-806 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tadashi Hara et al.: "Investigation of the Complex Formation Between Cobalt(III) and Bovine Serum Albumin by Use of Chemiluminescence Detection." THE SCI.AND ENG.REVIEW OF DOSHISHA UNIV.29. 1-6 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tadashi Hara et al.: "Chemiluminescence of Lーtryptophan in Copper(II) ーIron(II) ーHydrogen Peroxide ー Potassium Sodium Tartrate System." THE SCI.AND ENG.REVIEW OF DOSHISHA UNIV.31. 303-310 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tadashi Hara: "Chemiluminescence of LーTryptophane on the Copper(II)ーHydrogen peroxideーPotassium Sodium Tartrate System." Chem.Lett.843-846 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Tadashi Hara: "The Determination of a Small Amount of a Biological Constituent by Use of Chemiluminescence.XVI.High Sensitive Detection of Proteins by an IonーExchange ChromatographーChemiluminescence Detector System." Bull.Chem.Soc.Jpn.63. 770-776 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Tadashi Hara: "The Determination of a Small Amount of a Biological Constituent by the Use of Chemiluminescence.XVII.Experimental Consideration on the Chemiluminescence Detection of Protein Using the 1,10ーPhenanthrolineーHydrogen PeroxideーCopper(II)System." Bull.Chem.Soc.Jpn.63. 2423-2425 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Tadashi Hara: "Chemiluminescence Analyses of Biological Constituents Using MetalーComplex Catalysts." Anal.Sci.6. 797-806 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 塚越一彦: "化学発光反応を用いる微量タンパク質のフロ-インジェクション分析法の改良" 分析化学. 38. T100-T103 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Tadashi Hara: "The Determination of a Small Amount of Biological Constituent by the Uue of Chemiluminescence.XV.A Zeolite Column-CL Detection System for Separation and Determination of…." Bull.Chem.Soc.Jpn.62. 1501-1508 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Tadashi Hara: "A Highly Sensitive Fiber-Optic Immunosensor Using a Metal-Complex Compound as a Chemiluminescent Catalyst." Bull.Chem.Soc.Jpn.62. 2844-2848 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Tadashi Hara: "The Determination of a Small Amount of Biological Constituent by the Use of Chemiluminescence.XIII.Experimental Consideration on the Chemiluminescence Detection of Protein Using the 1,10-phenanthroline…." Bull.Chem.Soc.Jpn.

    • Related Report
      1989 Annual Research Report
  • [Publications] Tadashi Hara: "Chemiluminescence of L-Tryptophane on the Copper(II)-Hydrogen Peroxide-Potassium Sodium Tartrate Alkaline System." Chem.Lett.

    • Related Report
      1989 Annual Research Report
  • [Publications] 塚越一彦: 同志社大学理工学研究報告. 29. 1-6 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Tadashi Hara.: Bull.Chem.Soc.Jpn.61. 2779-2783 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Tadashi Hara.: Bull.Chem.Soc.Jpn.61. 2996-2998 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Tadashi Hara.: Bull.Chem.Soc.Jpn.

    • Related Report
      1988 Annual Research Report
  • [Publications] 塚越一彦: 分析化学.

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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