Chromatographic Separation of Proteins Using chitosan Ion Exchangers
| Project/Area Number |
63550715
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
化学工学
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
YOSHIDA Hiroyuki University of Chemical Osaka Prefecture, Engineering, Assistant Professor, 工学部, 講師 (50081360)
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| Project Period (FY) |
1988 – 1989
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1989: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1988: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | Chitosan / Chitin / Ion Exchanger / Protein / Adsorption / Separation / Chromatography / BSA / 牛血清アルブミン / クロマトグラフィ |
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| Research Abstract |
Large-scale chromatographic separation of proteins using the chitosan ion exchangers, which may be made very cheaply from the shells of arthropods, appears technically and economically feasible. Details of the conclusions are as follows :
1. The adsorption of bovin serum albumin (BSA) on the crosslinked chitosan appears technically feasible for salt-containing solutions. When inorganic elecLrolyLes coexisted in the solulioii, Llie equilibrium isol[ierms were liLLIe affected boti by the concentration of the salt and by pil. BSA adsorbed on the resin can be eluted by buffer solution of pH=9.
2. The strong base crosslinked chitosan adsorbed BSA well when there were no inorganic electrolytes except for buffer in the solution. The equilibrium isotherms were affected by pH considerably. When PH=7, the amount of adsorption was largest. BSA adsorbed on the resin- was eluted by NaCl aqueous solution.
3. The intraparticle effective diffusivity of BSA was affected by the concentration of BSA and pH. When the concentration of BSA is lower than I g/dm^3, the uptake data agreed well with the parallel transport mechanism of surface and pore diffusions. When the concentration of BSA is higher than I g/dm^3, the pore diffusion was the rate controlling step and shrinking core model agreed well with the data.
4. Breakthrough curves and elution curves were measured. Since the ion exchange particles are hard, the solution flowed through the bed well even when the bed was long. The experimental breakthrough curves agreed well with the analytic solution presented by us[Yoshida et al ; Chem. Eng. Sci., 39, 1489(1984)]. The intraparticle diffusivities determined from the breakthrough curves agreed with those determined by the shallow bed method.
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Report
(3 results)
Research Products
(19 results)
