| Project/Area Number |
63550723
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
反応工学
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| Research Institution | Nagoya University |
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Principal Investigator |
IIJIMA Shinji Dept. of Chemical Engineering, Nagoya University, Associate professor, 工学部, 助教授 (00168056)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Takeshi Department of Chemical Engineering, Nagoya University, Professor, 工学部, 教授 (10043324)
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| Project Period (FY) |
1988 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1989: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1988: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Animal cells / Hybridoma / Immobilization / Cellulose / Monoclonal antibody / Gel entrapment / Alginate / Bioreactor / 胞括固定 / モノクローナル抗体 |
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| Research Abstract |
In order to improved the production of useful biochemicals by animal cell culture, a novel immobilization methods have been developed. Two hybridoma cell lines that produced anti- erythropoietin and anti-alpha -amylase monocional antibody were entrapped in alginate gel and then the immobilized animal cell gel was covered with urethane polymer. By this method of immobilization, more than 10^7 cells/ml gel was obtained and the volumetric productivity of the antibody increased almost 10 times comparing to the regular cultures.
In order to culture anchorage depended cells, a novel macroporous cellulose fiber(SIC) was used. Transformed human endothelial cells were immobilized on this support and cultured in a fluidized bed reactor. By optimizing medium and oxygen supply, high concentration of cells more than 10 cells/ml. SIC could be obtained. By introducing a charged group on the surface of SIC, hybridoma cell was also immobilized and high concentration of cells near 10^8 cells/ml. SIC was attained.
By regular cultivation of animal cells, oxygen supply is the most serious problem. Therefore, we compared several method and found that enough amount of oxygen for the growth of high concentration of cells more than 10^7 cell/ml could be supplied by using perfluorochemicals.
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