| Project/Area Number |
63560001
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Breeding science
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| Research Institution | Chiba University |
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Principal Investigator |
MII Masahiro Chiba Univ. Hort, Assoc, Prof., 園芸学部, 助教授 (30093074)
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| Project Period (FY) |
1988 – 1989
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1989: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1988: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | carrot / Daucus carota / somatic embryo / hybrid / asymmetrical fusion / cytoplasmic male sterility / iodoactamide / X-ray / Carrot / Somatic embryo / Cybrid / Asymmetrical fusion / Cytoplasmic male sterility / Iodoactamide / 細胞融合 / 雄性不稔 / 細胞質置換 / プロトプラスト / ポリエチレングリコール / 電気融合 |
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| Research Abstract |
Introduction of cytoplasmic male sterility into normal fertile plants and vice versa were tried by using asymmetrical protoplast fusion technique and incorporation of isolated nuclei in carrot.
Both donor and recipient cell lines of cytoplasm were fused by PEG treatment after irradiation with 60 kR x-ray and treatment with 10 mM iodoactamide, respectively. Irrespective of the cell materials used, many plantlets were regenerated from the fusion products via somatic embryogenesis. However, no plants with altered male sterility were obtained. In the control cultures treated with only x-ray or with IOA, some escaped colonies were obtained presumably due to the complementation. Therefore, stronger treatments were applied to suppress the escape before fusion treatments. These treatments reduced the number of survived colonies which eventually developed into embryos. Further experiments are now in progress.
Isolation of nuclei from the embryogenic cell cultures was extremely difficult, whereas that from vacuolated non-embryogenic cells was relatively easy. However, the use of nuclei isolated from non-embryogenic cells was not suitable for obtaining plant regeneration after nuclei incorporation. Further experiment is required to get nuclei from embryogenic cells.
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