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Immunocytochemical analysis on localization of plant hormones in callus related to regeneration capaci

Research Project

Project/Area Number 63560002
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Breeding science
Research InstitutionThe University of Tokyo

Principal Investigator

YOSHIDA Kaoru  The Univ. of Tokyo, Fac. of Agriculture, Instructor, 農学部, 助手 (70183994)

Project Period (FY) 1988 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1990: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1989: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1988: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsRice / Barley / Regeneration / Plant Hormone / Adventitious Shoot / Embryoid / Immunocytochemistry / Callus / 再分化能 / 不定根 / ジベレリン / サイトカイニン
Research Abstract

To clear the relation between regeneration capacity and localization of plant hormones in callus, a culture system of adventitious shoot formation and embryogenesis from cell suspension of rice (Oryza sativa L.) was established and immunocytochemistry was applied.
When rice callus was transferred onto a regeneration medium containing low level of kinetin and ABA, it differentiated embryoid in high rate. On the other hand, when it was transferred onto a medium containing high level of 1-(2-chloro-4-pyridyl)-3-phenylurea (a kind of cytokinin) instead of kinetin, it differentiated adventitious shoots in high rate. Then, it is clear that regeneration pathway can be controlled by culture conditions.
To detect IAA and ABA localization, immunocytochemistry was applied to the rice culture system using anti-IAA or anti-ABA monoclonal antibody. Neither IAA nor ABA could be detected, because epitope sites considered to be broken by fixation treatment.

Report

(4 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] 吉田 薫: "オオムギ種子根カルスの染色体変異に及ぼす培養温度の効果" 育種学雑誌. 38(別冊2). 202-203 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 藤井 省吾・吉田 薫・武田 元吉: "イネ再分化時の培養条件による分化経路の制御" 育種学雑誌. 40(別冊2). 56-57 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 藤井 省吾・吉田 薫・武田 元吉: "イネ培養細胞の分化制御と組織学的観察" 育種学雑誌. 41(別冊1). (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yoshida, K.: "Effects of temperature on chromosome aberration in callus derived from barley root tip." Japan. J. Breed.38 (suppl. 2). 202-203 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Fujii, S., K. Yoshida and G. Takeda: "Control of regeneration pathway by culture condition from suspension callus of rice." Japan. J. Breed. 40 (suppl. 2). 56-57 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Fujii, S., K. Yoshida and G. Takeda: "Histological observations of rice culture during controlled regeneration." Japan. J. Breed. 41 (suppl. 1). (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 藤井 省吾・吉田 薫・武田 元吉: "イネ再分化時の培養条件による分化経路の制御" 育種学雑誌. 40(別冊2). 56-57 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 藤井 省吾・吉田 薫・武田 元吉: "イネ培養細胞の分化制御と組織学的観察" 育種学雑誌. 41(別冊1). (1991)

    • Related Report
      1990 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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