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Molecular genetic analysis of genes involved in melanin biosynthesis in plant pathogenic fungi utilizing its transformation system

Research Project

Project/Area Number 63560044
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 植物保護
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

KUBO Yasuyuki  Kyoto University, Faculty of Agriculture, Research Associate, 農学部, 助手 (80183797)

Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1989: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1988: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsPathogenicity / Melanin / Plant Pathogenic Fungi / Molecular Genetics / Transformation / 遺伝子解析
Research Abstract

In this research, transformation of a Plant Pathogenic funongi was constructed and a gene concerning melanin biosynthesis, one of the pathogenic factors, of Colletotrichum lagenarium was cloned. The results are as follow.
1. Protoplast system of C.lagenarium , which can be used for both isolation of genomic DNA and reception of foreign DNA, was constructed. 2. Benomyl resistant beta-tubulin gene was cloned from benomyl resistant C.lagenarium mutant. The gene was utilized as a selection marker for transformation of this fungus. 3. Cosmid vector for construction of genomic DNA library of C. lagenarium was constructed using resistant beta-tubulin gene as a selection marker. 4. Genomic DNA library of C.lagenarium was constructed in the cosmid vector. 5. Genomic library concerning melanin biosynthesis was screened by transformation of an albino mutant by the genomic library DNA. 6. A gene determining the phenotype of albino mutant was cloned by isolating the cosmid DNA from a melanin restored transformant.

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Chihiro Tanada: "Genetic analysis and characterisation of Cochlibolus heterostrophus colour mutants" Mycological Reseach. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Yasuyuki Kubo: "The Fungel Spore and Initiation in Plants and Animals" Plenum Publishing Corporation, (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kubo, Y. and Furusawa, I.: "The Fungal Spore and Disease Initiation in Plants and Animals" Plenum Publishing Corporation, 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Tanaka, C., Kubo, Y., Tsuda, M.: "Genetic analysis and characterization of Cochliobolus heterostrophus color mutants" Mycological Research, 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Chihiro Tanaka: "Genetic analysis and characterisation of Cochliobolus heterostrophus colour mutants" Mycological Research. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yasuyuki Kubo: "The Fungal Spore and Disease Initiation in Plants and Animals" Plenum Publishing Corporation, (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kubo,Y.: Expenmental Mycology. 13. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Tajima,S.: Experimental Mycology. 13. (1989)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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