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IDENTIFICATION OF IAA GENES AND ITS REGULATION IN TAPHRINA WIESNERI

Research Project

Project/Area Number 63560046
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 植物保護
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

YAMADA Tetsuji  OKAYAMA UNIV. ASSIS. PROFESSOR, 農学部, 助教授 (00191320)

Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1989: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1988: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsTaphrina wiesneri / Taphrina deformans / Taphrina pruni / IAA / Hypertrophy / TUMORIGENICITY / モモ縮葉病菌 / 腫瘍形成 / オーキシン / トリプトファントランザミネース
Research Abstract

Similarly to the cases of plant bacterial pathogen, such, as Pseudomonas savastanoi and Agrobacterium tumefaciens, plant fungal hyperplastic pathogen, Taphrina wiesneri, Taphrina deformans and Taphrina pruni, produced high concentrations of IAA and cytokinin that seems to result in disturbing the hormone balance in the host cells followed by the hyperplasia of plant cells.
In this research, we elucidated the IAA biosynthetic pathway of Taphrina wiesneri. and purified one of relative enzyme of this pathway to investigate the regulational mechanisms of IAA biosynthesis. Further, we obtained Iaa- mutants and carried out pulse field gel electrophoresis for the genetic analysis of the mutants.
1. <Biostnthetic pathway of IAA and their regulation> There are two biosynthetic pathway of IAA in Taphrina wiesneri, namely 1) from L-tryptophan (Trp), indole-3-pyruvic acid (IPA), indole-3-acetaldehyde (IAAL) to IAA, 2) indole-3-aceton-itrile (IAN) to IAA. Although tryptophan amino transferase (TATase … More ) which convertes Trp into IPA is uninducible, IAN nitrilase which :convertes IAN into IAA is inducible. One of the IAA biosynthetic pathways was regulated by the oxidation or reduction of IAA1, because in clofibric acid (CFA) mutant of Taphrina wiesneri (Iaa- mutant) IAA was not synthesized from IAA1, and indole-3-ethanol (IEOH) was accumulated.
2. <Purification of TATase> It was clarified that at least three TATase isozymes are present by the purification of these enzymes using arhmonium sulfate precipitation, gel filtration, DEAE ion exchange, and affinity chromatography.
3. <Selection of IAA- mutant> IAA deficient mutant was selected by the resistance against anti-auxin compound, CFA. In this mutant, IAA producibility was significant inhibited, and IEOH accumulation was observed instead of IAA. Therefore it was speculated that the treatment with CFA might have caused some mutation in gene encoding IAAL monooxigenase. In addition, we observed that chromosome III (a tentative name) was separated into two fragments by the pulse field gel electrophoresis. Less

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Yamada,T.et al.: "Suppression of pisatin,phenylculanene ammonia-lycise mRNA,and chalcone,synthase mRNA accuulation by a putative pathogenicity facter from the fungus M.Pinodes" Molecular Plant Microbe Interaction. 2. 256-261 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Yamada,T.et al.: "Elicitor-like substances present in barley and wheat seeds." J.Phytopath. 128. 89-98 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Shiraishi et al.: "Infection enhancing factor foe Erisiphe graminis prepared from barley seadlings" Aun.Phytopath.Soc.Jpn. 55. 357-360 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] T.Yamada,et al.: "Detection of indoleacitic and brosynthesis in some species of Taphrina causing hypertrophic diseases in plants." Subpitted to Ann Phytopath.soc.Jpn.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Gaffney,T.D.et al.: "The indoleacetic acid operon of Pseudomonas savastanoi:Transcription analysis and primoter identification" submitted to J.Bacteriol.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] T.Yamada,et al.: "Changes in indoleacetic acid producting and chromiosome length polymorphism in clofibric and resis tant mutants of Taphrina wiesueri and T.defonans" subritted to Curr.Genetics.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 山田哲治: "オリ-ブがん腫病の病原性遺伝子解析『植物病害と遺伝子工学』" 朝日出版, 8 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 山田哲治: "高等植物の情報発現と制御" 丸善, 204-224 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Tetsuji Yamada: "Suppression of pisatin, phenylalanine ammonia-lyase mRNA and chalcone synthase mRNA accumulation by a putative pathogenicity factor from Mycosphaerella pinodes." Mol. Plant-Micr. Intr.2. 256-261 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Tetsuji Yamada: "Elicitor-like substances present in barley and wheat seeds." J. Phytopath, 128:89-98 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Tomonori Shiraishi: "Infection inducing factor for Erisiphe graminis prepared from barley seedlings." Ann. Phytopath. Soc. Jpn 55: 357-360, 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Yamada, T., Tsukamoto, H., Shiraishi, T., Nomura, T., Otsuka, H., and H. Oku: "Detection of indoleacetic acid biosynthesis in some species of Taphrina causing hypertrophic diseases in plants." Ann. Phytopath. Soc. Jpn.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Yamada, T., Tsukamoto, H., Shiraishi, T., Kawamata, S., and H. Oku: "Changes in indoleacetic acid production and chromosome length polymorphism in clofibric acid resistant mutants of Taphrina wiesneri and T. deformans." Curr. Genetics.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Gaffney, T.D., Silva, O.C., Yamada, T., and T. Kosuge: "The indoleacetic acid operon of Pseudomonas syringae subsp. savastanoi: Transcription analysis and promoter identification." J. Bacteriol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Yamada,T.et al: "Suppression of pisatin,pheuylclanine ammonia-lycse mRNA,and chalcone,synthase mRNA,accumnlation by a putative pathogenicity faitor from ttce fungus M.pinodes" Molecular Plant Microle Interaction. 2. 256-261 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yamada,T.et al: "Eliutor-lcke substances present in barley and wheat seeds." J.phytopath.128. 89-98 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Shiraishi et al: "Infection enhancing factor for Erisiphe graminis prepared from barley seedlings" Aum.Phytopath.Soc.Jpn.55. 357-360 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Yamada,et al: "Detection of indoleacitic acid biosynthesis in seme species of Taphrina causing hypertropbic diseases in plants" subnitted to Ann.Phytopath.Soc.Jpn.

    • Related Report
      1989 Annual Research Report
  • [Publications] Gaffney,T.D.et al: "The indole acetic acid operon of Pseudomenas sarastanoi:Transcription analysis and promoter identification" submtted to J.Bacteriol.

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Yamada,et al: "Changes in indoleacetic acid production and chiomosome length polymorphism in clofibrec acid resis tant mutants of Japhriua wiesueri and T.cleforuaus" submitted to Curr.Genetics.

    • Related Report
      1989 Annual Research Report
  • [Publications] 山田哲治: "オリ-ブがん腫病の病原性遺伝子解析『植物病害と遺伝子工学』" 朝日出版, 8 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 山田哲治: "高等植物の情報発現と制御" 丸善, 204-224 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 大植美香、塚本浩史、山田哲治、白石友紀、奥八郎: 日本植物病理学会報.

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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