| Project/Area Number |
63560060
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
土壌・肥料
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| Research Institution | The University of Tokyo |
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Principal Investigator |
YOSHIMURA Etsuro The Univ. of Tokyo, Agric., Instructor, 農学部, 助手 (10130303)
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| Co-Investigator(Kenkyū-buntansha) |
HAMADA Noriaki The Univ. of Tokyo, Agric., Instructor, 農学部, 助手 (20172976)
OHKUBO Akira The Univ. of Tokyo, Agric., Instructor, 農学部, 助手 (20111479)
YAMAZAKI Sunao The Univ. of Tokyo, Agric., Assoc. Professor, 農学部, 助教授 (00011982)
TODA Shozo The Univ. of Tokyo, Agric., Professor, 農学部, 教授 (40011845)
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| Project Period (FY) |
1988 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1989: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1988: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Plant / Heavy metal / induced peptide / Phytochelatin / 重金属誘導のペプチド |
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| Research Abstract |
1. Purification of Phytochelatin(PC) Z. mays was cultivated in Arnon-lioagland solution containing 3 pM Cd. The root was homogenized with extraction buffer and Cd- containing substance was extracted and purified via gel- filtration and ion exchange chromatographyes. Amino acid analysis of this substance showed Glu:Cys:Gly was 5.9:4.6:1.0, N-terminal and C-terminal amino acids of the substance were indicated to be Glu and Gly, respectively. These data showed Cd-binding substance from Z. mays was composed of PC.
2. Determination of PC Analytical method for PC was developed as follows. PCs were separated by HPLC, added by 5,5'-dithiobis(2-nitrobenzoic acid) and detected by the absorption at 412nm. The purified Cd-binding substance was analyzed by this method and shown to consist of seven PCs with different gamma-glutamylcysteine unit. The similar chromatograms were also obtained from the extracts of toots and leaves of Z.mays which were cultivated in Cd-containing medium.
3. Detoxification of heavy metals by PC Z.mays was cultivated with Cd and buthioninsulfoximine(BSX). While the growth of the plant was normally in the medium containing Cd or BSX, it was inhibited in the medium containing Cd and BSX. PC was detected with a significant amount in the root cultivated with Cd containing medium, but not detected with the medium containing Cd and BSX. These results indicated that PC was playing an important role on detoxification of heavy metals and that gamma-glutamylcysteine and/or glutatbione could be precursor for PC synthesis.
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