| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1989: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1988: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Research Abstract |
1. A free ceramide was isolated from membrane lipids of Z. mobilis and its molecular species was shown to be N-2-palmitoyl-1,3-dihydroxyhexadecane. 2. A PE N-methyltransferase-defective mutant was isolated from Z. mobilis, which was completely defective in all the three enzyme activities dependent on PE, PMME and PDME. Two revertants derived from the mutant recovered all the three enzyme activities to nearly the same levels of the wild type strain, suggesting that in Z. mobilis the three-step methylation in the pathway would be catalyzed by a single enzyme.
3. A mutant deficient in tetrahydroxybacteriohopane (THBH) biosynthesis was isolated from Z. mobilis as a nystatin-resistant mutant. Quantitative analysis showed that the THBH content was reduced to about 20 % of that of the parent strain. The mutant exhibited to be sensitive to a high glucose concentration but tolerant to a high ethanol concentration, suggesting that in Z. mobilis the THBH might be responsible for tolerant to the former.
4. A THBH-increased mutant was isolated from Z. mobiles, of which the THBH content was increased to about 3 times of that of the parent strain. Quantitative analysis of THBH, glucosamine-THBH and carbocyclic pseudopentose-THBH, in THBH-defective mutant and THBH-increased mutant suggests that the side-chains of those hopanoids, tetra- hydroxypentane, trihydroxypentenyl glucosamine and trihydroxypentenyl carbocyclic pseudopentose, would be independently incorporated into the hopane skeleton in hopanoid biosynthesis.
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