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Colloidal Calcium Phosphate-cross-linkage in Casein Micelles.

Research Project

Project/Area Number 63560134
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 製造化学・食品
Research InstitutionKagoshima University

Principal Investigator

AOKI Takayoshi  Kagoshima Univ. Agriculture Associate Professor, 農学部, 助教授 (70034460)

Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1989: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1988: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsCasein / Casein micelle / Calcium phosphate / High performance gel chromatography / Heating / Cooling / beta-Casein / 高速液体クロマトグラフィ / コロイド状リン酸カルシウム / 高速液体ゲルクロマトグラフィー
Research Abstract

It is recognized that colloidal calcium phosphate (CCP) participates in characteristics of casein micelles in milk processing such as heating, cooling and freezing. However, the state and role of CCP has not yet been elucidated sufficiently. In the present study, the effects of heating on CCP-cross-linkage and the relation between CCP-cross-linkage and the release of serum casein on cooling were examined.
It was confirmed that CCP content was increased on heating casein micelle dispersion (CMD) and concentrated CMD at 60-90 ゚C for 10 min or 135-140゚C for 15-75 sec. The content of casein aggregates cross-linked by CCP was decreased from 51.9 to 46.1% in CMD and 52.5 to 43.6% in concentrated CMD on heating at 135-140 C for 75 sec, indicating the cleavage of the CCP-cross-linkage.
CCP-increased CMD was prepared by addition of calcium and phosphate. The content of casein aggregates cross-linked by CCP was increased from 49.3 to 68.3%. The amount of serum casein released from casein micelles was decreased with increasing CCP content. When the CCP content was increased to a large extent, beta-casein released on cooling was decreased from 31.8 to 2.1% of the total casein while beta-casein cross-linked by CCP and beta-casein which is not cross-linked and not released on cooling were increased from 46.2 to 69.9% and from 22.0 to 28.0% of the total beta-casein, respectively. The increase in beta-casein cross-linked by CCP and strengthening of interaction between beta-casein and other casein constituents were considered to be reasons for the decrease of beta-casein release on cooling.

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Takayoshi Aoki,Taketoshi Umeda and Yoshitaka Kako: "Cleavage of the linkage between colloidal calcium phosphate and casein on heating at high temperature." Journal of Dairy Research.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Takayoshi Aoki,Nagisa Yamada and Yoshitaka Kako: "Relation between colloidal calcium phosphate cross-linkage and release of β-casein from bovine casein micelles on heating." Agric Biol.Chem.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Takayoshi Aoki, Taketoshi Umeda and Yoshitaka Kako: "Cleavage of the linkage between colloidal calcium phosphate and casein on heating at high temperature." Journal of Dairy Research.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Takayoshi Aoki, Nagisa Yamada and Yoshitaka Kako: "Relation between colloidal calcium phosphate-cross-linkage and release of beta-casein from bovine casein micelles on cooling." Agric. Biol. Chem.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Takayoshi Aoki,Taketoshi Umeda and Yoshitaka Kako: "Cleavege of the linkage between colloidal calcium phosphate and casein on heating milk at high temperature." Journal of Dairy Research.

    • Related Report
      1989 Annual Research Report
  • [Publications] Takayoshi Aoki,Nagisa Yamada and Yoshitaka Kako: "Relation between colloidal calcium phosphate cross-linkage and releaseof β-casein from bovine casein micelleson cooling." Agric.Biol.Chem.

    • Related Report
      1989 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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