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Investigation of white clover ecotypes showing high nitrogenase activity under an abundant nitrogen and applying them to mixed grassland.

Research Project

Project/Area Number 63560251
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 畜産学(含草地学)
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

SUGAWARA Kazuo  Faculty of Agriculture, Tohoku University, Associate Professor., 農学部附属草地研究施設, 助教授 (20005672)

Co-Investigator(Kenkyū-buntansha) ISAWA Takeshi  University farm, Faculty of Agriculture, Tohoku University,, 農学部附属農場, 教務職員 (70159656)
OOTAKE Hideo  Faculty of Agriculture, Tohoku University, Assistant., 農学部附属草地研究施設, 助手 (20191942)
Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1989: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1988: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsWhite clover / Nitrogen fixation / Adaptability for N fertilizer / Ecotype / Mixed grassland / シロクローバ / マメ科牧草 / 根粒菌
Research Abstract

(1). Ninety eight of white clover ecotypes were mainly collected from Tohoku district in Japan. The variations of some characteristics were identified among the collected white clovers.
(2). From variation pattern of nitrogenase activity after an application of nitrogen fertilizer, white clovers were grouped into 5 types, i.e., 0 type; no decrease of activity, AA type: quick decrease and quick recovery, AB type: quick decrease and slow recovery, BA type: slow decrease and quick recovery, and BB type: slow decrease and slow recovery. Thus, the response of nitrogenase activity to the nitrogen application differs among white clovers.
(3). The peroxidase isozyme of white clover appears in 6 bands. White clovers collected were grouped into a,b,c,d type by the different pattern of bands. The d type of isozyme pattern was closely related to 0 type of nitrogenase activity.
(4). In the mixed grassland, white clover and orchard grass, the inhibition of nitrogenase activity after nitrogen application was reduced compared with white clover grassland. This is assumed as the result of the absorption of nitrate nitrogen in soil by grass species.
(5). Under field condition, white clover shows the three peaks of nitrogenase activity in the year, May, July and September to October. The pattern of the seasonal variation of activity did not show significant correlation with mean temperature, sunshine hours and precipitation and N% in soil.
(6). From the mentioned above, there are white clover ecotypes showing high nitrogenase activity under an abundant nitrogen and 14 of the ecotypes like that were collected in this investigation, and the nitrogenase activity of white clover will be kept higher by the improvement of nitrogen circumstance in soil with mixing culture with grass species.

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] 菅原和夫: "白クロ-バによる施肥窒素の利用と共生的窒素固定" 日本草地学会誌. 34(別). 31-32 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 菅原和夫: "白クロ-バの窒素固定能におよぼす施肥窒素の影響-自生白クロ-バ間の比較-" 日本草地学会誌. 35(別). 47-48 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] K. SUGAWARA: "Seasonal variation in symbiotic nitrogen fixation of white clover" XVI International Grassland Congress. 1. 133-134 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 菅原和夫: "自生白クロ-バの窒素固定能耐肥性とPODアイソザム変異" 日本草地学会.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 菅原和夫: "自生白クロ-バの施肥窒素に対する窒素固定活性の感応型について" 日本草地学会.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kazuo Sugawara: "The utilization of applied nitrogen and symbiotic nitrogen fixation of white clover" J. Japan. Grassl. Sci. 34(Sup.) 31 32, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kazuo Sugawara: "Effect of applied nitrogen on nitrogen fixation of white clover Comparison among white clover ecotypes" J. Japan. Grassl. Sci. 35(Sup.) 47 48, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kazuo Sugawara: "Seasonal variation in symbiotic nitrogen fixation of white clover." XVI International Grassland Congress, 1, 133 134, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kazuo Sugawara: "POD isosyme variation and nitrogenase activity of white clover"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kazuo Sugawara: "POD isosyme variation and nitrogenase activity of white clover" "Response pattern of nitrogenase activity of white clover to applied nitrogen."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] K.SUGAWARA: "Seasonal variation in symbiotic nigrogen fixation of white clover" XVI International Grassland Congress. 1. 133-134 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 菅原和夫: "自生白クロ-バの窒素固定能耐肥性とPODアイソザイム変異" 日本草地学会.

    • Related Report
      1989 Annual Research Report
  • [Publications] 菅原和夫: "自生白クロ-バの施肥窒素に対する窒素固定活性の感応型について" 日本草地学会.

    • Related Report
      1989 Annual Research Report
  • [Publications] 菅原和夫: 日本草地学会誌. 34(別). 31-32 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 菅原和夫: 日本草地学会誌. 35(別). (1989)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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