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Chromatographic Analysis of Endogenous Factor Promoting the Color Formation in Porcine Muscle Sarcoplasm

Research Project

Project/Area Number 63560280
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 畜産化学
Research InstitutionAzabu University

Principal Investigator

NAGATA Yukiharu  Azabu University, School of Veterinary Medicine, Professor, 獣医学部, 教授 (30038202)

Co-Investigator(Kenkyū-buntansha) SAKATA Ryoichi  Azabu University, School of Veterinary Medicine, Assistant Professor, 獣医学部, 講師 (10153892)
Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1989: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1988: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsProcessed meat products / Color formation / Cooked cured meat color / Sarcoplasm / Nitrite / Curing
Research Abstract

The color formation of cooked cured meat products involves basically reactions of muscle myoglobin (Mb) with added nitrite, resulting in the formation of nitrosomyoglobin (NOMb). In recent years, the so-called cutter curing method has generally been used in meat processing plants to shorten curing time for manufacturing emulsion-type sausages. In this case, the meat is cut immediately following the addition of nitrite and other curing agents. Still, the meat takes on a favorable red color similar to that by conventional curing, such as the wet curing method. Previous studies indicate endogenous muscle components, especially in low-molecular weight sarcoplasm(LMS) fraction, to rapidly and non-enzymatically promote this color reaction during cooking. The present work was undertaken to determine the effective endogenous factor essential to the color formation of cooked cured meat products, and clarify its mode of action.
The endogenous factor promoting the color formation was separated from the LMS fraction of porcine skeletal muscle by Sephadex G-15 gel and ion exchange chromatography. The fraction with strong color forming ability was found to have a molecular weight range from 200-550 daltons, to be rich in peptide-like substances. The promoting action of the LMS fraction on the color formation was also shown to be caused by the acceleration of heat denaturation of heme pigments. The active subfraction of LMS fraction was further separated, and a peptide consisting of three amino acids may be one of the endogenous factors, Conditions of the high-performance liquid chromatography (HPLC) for analysis of the LMS fraction were investigated, and the active components obtained by gel chromatography could be divided into 7-8 parts with gradient solvent system consisting of trifluoroacetic acid-acetonitrile.

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Ryoichi Sakata and Yukiharu Nagata: "Chromatographische Analyse des in gekochtem,gepokeltem Schweine fleisch die Farbbildung fordernden endogenen Faktors" Fleischwirtschaft. 68. 394-397 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ryoichi Sakata and Yukiharu Nagata: "Fordernde Wirkung der niedermolekularen Sarkoplasmafraktion auf die Farbbildung" Fleischwirtschaft. 68. 1202-1205 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ryoichi Sakata and Yukiharu Nagata: "Chromatographic analysis of the endogenous factor promoting the color formation of cooked cured pork" Fleischwirtschaft international. 1988(2). 44-47 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ryoichi Sakata and Yukiharu Nagata: "Promoting action of low-molecular weight sarcoplasm fraction on color formation" Fleischwirtschaft international. 1989(1). 54-58 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Yukiharu Nagata and Ryoichi Sakata: "Mechanism of the action of an endogenous factor for promoting the color formation of cooked cured pork" Fleischwirtschaft.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ryoichi Sakata and Yukiharu Nagata: "Chromatographische Analyse des in gekochtem, gepokel tem Schweinefleisch die Farbbildung fordernden endogenen Faktors" Fleischwirtschaft, 68-3, 394-397, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ryoichi Sakata and Yukiharu Nagata: "Fordernde Wirkung der niedermolekularen Sarkoplasmafraktion auf die Farbbildung" Fleischwirtschaft, 68-9, 1202-1205, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ryoichi Sakata and Yukiharu Nagata: "Chromatographic analysis of the endogenous factor promoting the color formation of cooked cured pork" Fleischwirtschaft, international, 1988-2, 44-47, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ryoichi Sakata and Yukiharu Nagata: "Promoting action of low-molecular weight sarcoplasm fraction on color formation" Fleischwirtschaft, international, 1989-1, 54-58, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Yukiharu Nagata and Ryoichi Sakata: "Mechanism of the action of an endogenous factor for promoting the color formation of cooked cured pork" Fleischwirtschaft.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ryoichi Sakata and Yukiharu Nagata: "Promoting action of low-molecular weight Sarcoplasm fraction on color formation" Fleischwirtschaft international. 1989(1). 54-58 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yukihara Nagata Ryoichi Sakata: "Mechanism of the action of an endogenous factor for promoting the cokir formation of cooked cured pork" Fleischwirtschaft.

    • Related Report
      1989 Annual Research Report
  • [Publications] 永田致治・坂田亮一: Fleischwirtschaft.

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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